PMID- 27565895
OWN - NLM
STAT- MEDLINE
DCOM- 20170614
LR  - 20191210
IS  - 1471-4159 (Electronic)
IS  - 0022-3042 (Linking)
VI  - 139
IP  - 5
DP  - 2016 Dec
TI  - beta-Caryophyllene protects in vitro neurovascular unit against oxygen-glucose 
      deprivation and re-oxygenation-induced injury.
PG  - 757-768
LID - 10.1111/jnc.13833 [doi]
AB  - beta-Caryophyllene (BCP) mediates neuroprotection in cerebral ischemic animals. The 
      neurovascular unit (NVU) acts as an intricate network to maintain the neuronal 
      homeostatic microenvironment. However, the effects exerted by BCP on NVU remain 
      unclear. Therefore, we established an in vitro NVU model to investigate the 
      effects of BCP on oxygen-glucose deprivation and re-oxygenation (OGD/R)-induced 
      injury. This model involved the co-culture of brain microvascular endothelial 
      cells, neurons, and astrocytes. BCP (10 mumol/L) was applied for 24 h prior to 
      OGD/R and maintained throughout OGD/R. Blood-brain barrier (BBB) integrity and 
      neuronal apoptosis were analyzed. BCP pre-treatment prior to the initiation of 
      OGD/R significantly (i) decreased BBB permeability and neuronal apoptosis, (ii) 
      mitigated oxidative stress damage and the release of inflammatory cytokines, 
      (iii) down-regulated Bax expression, metalloproteinase-9 activity and expression, 
      and (iv) up-regulated claudin-5, occludin, ZO-1, growth-associated protein-43 and 
      Bcl-2 expression. Thus, BCP pre-treatment exerted multiple protective effects on 
      NVU in the context of OGD/R-induced injury. These protective effects potentially 
      occur via reductions in oxidative stress damage and inflammatory cytokines that 
      induce BBB breakdown, subsequently resulting in reduced neuronal apoptosis. The 
      NVU serves as putative therapeutic targets for cerebral ischemia, and the results 
      of this study provide new insights for the application of BCP as a 
      neuroprotective agent.
CI  - (c) 2016 International Society for Neurochemistry.
FAU - Tian, Xiaocui
AU  - Tian X
AD  - Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, College of 
      Pharmacy, Chongqing Medical University, Yuzhong District, Chongqing, China.
FAU - Peng, Jianhua
AU  - Peng J
AD  - Department of Neurosurgery, the Affiliated Hospital of Southwest Medical 
      University, Luzhou, China.
FAU - Zhong, Jianjun
AU  - Zhong J
AD  - Department of Neurosurgery, the First Affiliated Hospital of Chongqing Medical 
      University, Chongqing, China.
FAU - Yang, Mei
AU  - Yang M
AD  - Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, College of 
      Pharmacy, Chongqing Medical University, Yuzhong District, Chongqing, China.
FAU - Pang, Jinwei
AU  - Pang J
AD  - Department of Neurosurgery, the Affiliated Hospital of Southwest Medical 
      University, Luzhou, China.
FAU - Lou, Jie
AU  - Lou J
AD  - Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, College of 
      Pharmacy, Chongqing Medical University, Yuzhong District, Chongqing, China.
FAU - Li, Minghang
AU  - Li M
AD  - Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, College of 
      Pharmacy, Chongqing Medical University, Yuzhong District, Chongqing, China.
FAU - An, Ruidi
AU  - An R
AD  - Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, College of 
      Pharmacy, Chongqing Medical University, Yuzhong District, Chongqing, China.
FAU - Zhang, Qian
AU  - Zhang Q
AD  - Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, College of 
      Pharmacy, Chongqing Medical University, Yuzhong District, Chongqing, China.
FAU - Xu, Lu
AU  - Xu L
AD  - Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, College of 
      Pharmacy, Chongqing Medical University, Yuzhong District, Chongqing, China.
FAU - Dong, Zhi
AU  - Dong Z
AD  - Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, College of 
      Pharmacy, Chongqing Medical University, Yuzhong District, Chongqing, China.
LA  - eng
PT  - Journal Article
DEP - 20160919
PL  - England
TA  - J Neurochem
JT  - Journal of neurochemistry
JID - 2985190R
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Polycyclic Sesquiterpenes)
RN  - 0 (Sesquiterpenes)
RN  - BHW853AU9H (caryophyllene)
RN  - IY9XDZ35W2 (Glucose)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Anti-Inflammatory Agents, Non-Steroidal/*pharmacology
MH  - Cell Hypoxia/drug effects/physiology
MH  - Cells, Cultured
MH  - Endothelium, Vascular/drug effects/*metabolism
MH  - Glucose/*deficiency
MH  - Microvessels/drug effects/metabolism
MH  - Neurons/drug effects/*metabolism
MH  - Neuroprotective Agents/pharmacology
MH  - Oxygen/*metabolism
MH  - Polycyclic Sesquiterpenes
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sesquiterpenes/*pharmacology
OTO - NOTNLM
OT  - Blood-brain barrier
OT  - neuronal apoptosis
OT  - neurovascular unit
OT  - oxygen-glucose deprivation and re-oxygenation
OT  - beta-caryophyllene
EDAT- 2016/08/28 06:00
MHDA- 2017/06/15 06:00
CRDT- 2016/08/28 06:00
PHST- 2016/06/15 00:00 [received]
PHST- 2016/08/15 00:00 [revised]
PHST- 2016/08/18 00:00 [accepted]
PHST- 2016/08/28 06:00 [pubmed]
PHST- 2017/06/15 06:00 [medline]
PHST- 2016/08/28 06:00 [entrez]
AID - 10.1111/jnc.13833 [doi]
PST - ppublish
SO  - J Neurochem. 2016 Dec;139(5):757-768. doi: 10.1111/jnc.13833. Epub 2016 Sep 19.