PMID- 27566357
OWN - NLM
STAT- MEDLINE
DCOM- 20180118
LR  - 20180118
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 936
DP  - 2016 Sep 14
TI  - DNA aptamers for selective identification and separation of flame retardant 
      chemicals.
PG  - 208-15
LID - S0003-2670(16)30803-0 [pii]
LID - 10.1016/j.aca.2016.06.040 [doi]
AB  - Polybrominated diphenyl ethers (PBDEs) are group of chemicals which are 
      representative persistent organic pollutants (POPs) and used as brominated flame 
      retardants for many consumer products. PBDEs were phased out since 2009 but are 
      still frequently observed in various environmental matrices and human body. Here, 
      we report ssDNA aptamers which bind to BDE47, one of the PBDE congeners commonly 
      found in various environmental matrices, and show affinity to other major tri-to 
      hepta- BDE congeners. The PBDE specific aptamers were isolated from random 
      library of ssDNA using Mag-SELEX. Two out of 15 sequences, based on their 
      alignment and hairpin loop structures, were chosen to determine dissociation 
      constant with BDE47 and showed from picomolar to nanomolar affinities (200 pM and 
      1.53 nM). The aptamers displayed high selectivity to the original target, BDE47, 
      and implying general specificity to PBDE backbone with varying affinities to 
      other congeners. Further, we showed that the use of two aptamers together could 
      enhance the separation efficiency of BDE47 and other BDE congeners when dissolved 
      in a solvent compared to use of single aptamer. These aptamers are expected to 
      provide a tool for preliminary screening or quick separation of PBDEs in 
      environmental samples prior to trace quantitative analysis.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Kim, Un-Jung
AU  - Kim UJ
AD  - Center for Environment, Health and Welfare Research, Korea Institute of Science 
      and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of 
      Korea.
FAU - Kim, Byoung Chan
AU  - Kim BC
AD  - Center for Environment, Health and Welfare Research, Korea Institute of Science 
      and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of 
      Korea; Department of Energy and Environmental Engineering, University of Science 
      and Technology (UST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of 
      Korea. Electronic address: bchankim@kist.re.kr.
LA  - eng
PT  - Journal Article
DEP - 20160630
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Flame Retardants)
RN  - 0 (Halogenated Diphenyl Ethers)
SB  - IM
MH  - Aptamers, Nucleotide/*chemistry
MH  - Environmental Monitoring
MH  - Environmental Pollution/*analysis
MH  - Flame Retardants/*analysis
MH  - Halogenated Diphenyl Ethers/*analysis
MH  - Molecular Structure
OTO - NOTNLM
OT  - BDE47
OT  - Brominated flame retardant (BFR)
OT  - Polybrominated diphenyl ethers (PBDEs)
OT  - Quick separation
OT  - Screening
OT  - ssDNA aptamers
EDAT- 2016/08/28 06:00
MHDA- 2018/01/19 06:00
CRDT- 2016/08/28 06:00
PHST- 2016/04/28 00:00 [received]
PHST- 2016/06/20 00:00 [revised]
PHST- 2016/06/23 00:00 [accepted]
PHST- 2016/08/28 06:00 [entrez]
PHST- 2016/08/28 06:00 [pubmed]
PHST- 2018/01/19 06:00 [medline]
AID - S0003-2670(16)30803-0 [pii]
AID - 10.1016/j.aca.2016.06.040 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2016 Sep 14;936:208-15. doi: 10.1016/j.aca.2016.06.040. Epub 2016 
      Jun 30.