PMID- 27566359
OWN - NLM
STAT- MEDLINE
DCOM- 20180118
LR  - 20180118
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 936
DP  - 2016 Sep 14
TI  - Aptamer-based biosensor for label-free detection of ethanolamine by 
      electrochemical impedance spectroscopy.
PG  - 222-8
LID - S0003-2670(16)30819-4 [pii]
LID - 10.1016/j.aca.2016.06.056 [doi]
AB  - A label-free sensing assay for ethanolamine (EA) detection based on 
      G-quadruplex-EA binding interaction is presented by using G-rich aptamer DNA 
      (Ap-DNA) and electrochemical impedance spectroscopy (EIS). The presence of K(+) 
      induces the Ap-DNA to form a K(+)-stabilized G-quadruplex structure which 
      provides binding sites for EA. The sensing mechanism was further confirmed by 
      circular dichroism (CD) spectroscopy and EIS measurement. As a result, the charge 
      transfer resistance (RCT) is strongly increased as demonstrated by using the 
      ferro/ferricyanide ([Fe(CN)6](3-/4-)) as a redox probe. Under the optimized 
      conditions, a linear relationship between DeltaRCT and EA concentration was obtained 
      over the range of 0.16 nM and 16 nM EA, with a detection limit of 0.08 nM. 
      Interference by other selected chemicals with similar structure was negligible. 
      Analytical results of EA spiked into tap water and serum by the sensor suggested 
      the assay could be successfully applied to real sample analysis. With the 
      advantages of high sensitivity, selectivity and simple sensor construction, this 
      method is potentially suitable for the on-site monitoring of EA contamination.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Liang, Gang
AU  - Liang G
AD  - Risk Assessment Lab for Agro-Products (Beijing), Ministry of Agriculture, PR 
      China; Beijing Municipal Key Laboratory of Agriculture Environment Monitoring, PR 
      China; Beijing Research Center for Agricultural Standards and Testing, Beijing 
      Academy of Agricuture and Forestry Science, Beijing 100097, PR China; State Key 
      Laboratory of Water Environment Simulation, School of Environment, Beijing Normal 
      University, Beijing 100875, PR China.
FAU - Man, Yan
AU  - Man Y
AD  - Risk Assessment Lab for Agro-Products (Beijing), Ministry of Agriculture, PR 
      China; Beijing Municipal Key Laboratory of Agriculture Environment Monitoring, PR 
      China; Beijing Research Center for Agricultural Standards and Testing, Beijing 
      Academy of Agricuture and Forestry Science, Beijing 100097, PR China.
FAU - Jin, Xinxin
AU  - Jin X
AD  - Risk Assessment Lab for Agro-Products (Beijing), Ministry of Agriculture, PR 
      China; Beijing Municipal Key Laboratory of Agriculture Environment Monitoring, PR 
      China; Beijing Research Center for Agricultural Standards and Testing, Beijing 
      Academy of Agricuture and Forestry Science, Beijing 100097, PR China.
FAU - Pan, Ligang
AU  - Pan L
AD  - Risk Assessment Lab for Agro-Products (Beijing), Ministry of Agriculture, PR 
      China; Beijing Municipal Key Laboratory of Agriculture Environment Monitoring, PR 
      China; Beijing Research Center for Agricultural Standards and Testing, Beijing 
      Academy of Agricuture and Forestry Science, Beijing 100097, PR China. Electronic 
      address: panlg@nercita.org.cn.
FAU - Liu, Xinhui
AU  - Liu X
AD  - State Key Laboratory of Water Environment Simulation, School of Environment, 
      Beijing Normal University, Beijing 100875, PR China. Electronic address: 
      xhliu@bnu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20160702
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Aptamers, Nucleotide)
RN  - 5KV86114PT (Ethanolamine)
SB  - IM
MH  - Aptamers, Nucleotide/*chemistry
MH  - *Biosensing Techniques
MH  - *Dielectric Spectroscopy
MH  - Ethanolamine/*analysis
OTO - NOTNLM
OT  - Aptamer
OT  - Biosensor
OT  - Electrochemical impedance spectroscopy
OT  - Ethanolamine
EDAT- 2016/08/28 06:00
MHDA- 2018/01/19 06:00
CRDT- 2016/08/28 06:00
PHST- 2016/04/01 00:00 [received]
PHST- 2016/06/28 00:00 [revised]
PHST- 2016/06/30 00:00 [accepted]
PHST- 2016/08/28 06:00 [entrez]
PHST- 2016/08/28 06:00 [pubmed]
PHST- 2018/01/19 06:00 [medline]
AID - S0003-2670(16)30819-4 [pii]
AID - 10.1016/j.aca.2016.06.056 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2016 Sep 14;936:222-8. doi: 10.1016/j.aca.2016.06.056. Epub 2016 
      Jul 2.