PMID- 27566664
OWN - NLM
STAT- MEDLINE
DCOM- 20170321
LR  - 20191210
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 121
IP  - 6
DP  - 2016 Dec
TI  - Strychnos pseudoquina A. St. Hil.: a Brazilian medicinal plant with promising 
      in vitro antiherpes activity.
PG  - 1519-1529
LID - 10.1111/jam.13279 [doi]
AB  - AIMS: To investigate the anti-HSV and anti-inflammatory effects of a standardized 
      ethyl acetate extract (SEAE) prepared with the stem bark of Strychnos 
      pseudoquina, along with two isolated compounds: quercetin 3-O-methyl ether (3MQ) 
      and strychnobiflavone (SBF). METHODS AND RESULTS: The mechanisms of action were 
      evaluated by different methodological strategies. SEAE and SBF affected the early 
      stages of viral infection and reduced HSV-1 protein expression. Both flavonoids 
      elicited a concentration-dependent inhibition of monocyte chemoattractant 
      protein-1 (MCP-1), whereas 3MQ reduced the chemokine release more significantly 
      than SBF. Conversely, both compounds stimulated the production of the cytokines 
      TNF-alpha and IL-1-beta in LPS-stimulated cells, especially at the intermediate and the 
      highest tested concentrations. CONCLUSIONS: SEAE and SBF interfered with various 
      steps of HSV replication cycle, mainly adsorption, postadsorption and 
      penetration, as well as with beta and gamma viral proteins expression; moreover, a 
      direct inactivation of viral particles was observed. Besides, both flavonoids 
      inhibited MCP-1 selectively, a feature that may be beneficial for the development 
      of new anti-HSV agents. SIGNIFICANCE AND IMPACT OF THE STUDY: The results 
      indicated that the samples present anti-HSV and anti-inflammatory activities, at 
      different levels, which is an interesting feature since cold and genital sores 
      are accompanied by an inflammation process.
CI  - (c) 2016 The Society for Applied Microbiology.
FAU - Boff, L
AU  - Boff L
AD  - Laboratorio de Virologia Aplicada, Programa de Pos-graduacao em Farmacia, 
      Universidade Federal de Santa Catarina (UFSC), Florianopolis, SC, Brazil.
FAU - Silva, I T
AU  - Silva IT
AD  - Laboratorio de Virologia Aplicada, Programa de Pos-graduacao em Farmacia, 
      Universidade Federal de Santa Catarina (UFSC), Florianopolis, SC, Brazil.
FAU - Argenta, D F
AU  - Argenta DF
AD  - Laboratorio de Virologia Aplicada, Programa de Pos-graduacao em Farmacia, 
      Universidade Federal de Santa Catarina (UFSC), Florianopolis, SC, Brazil.
FAU - Farias, L M
AU  - Farias LM
AD  - Departamento de Bioquimica e Biologia Molecular, Universidade Federal de Vicosa 
      (UFV), Vicosa, MG, Brazil.
FAU - Alvarenga, L F
AU  - Alvarenga LF
AD  - Departamento de Produtos Farmaceuticos, Faculdade de Farmacia, Universidade 
      Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
FAU - Padua, R M
AU  - Padua RM
AD  - Departamento de Produtos Farmaceuticos, Faculdade de Farmacia, Universidade 
      Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
FAU - Braga, F C
AU  - Braga FC
AD  - Departamento de Produtos Farmaceuticos, Faculdade de Farmacia, Universidade 
      Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
FAU - Leite, J P V
AU  - Leite JP
AD  - Departamento de Bioquimica e Biologia Molecular, Universidade Federal de Vicosa 
      (UFV), Vicosa, MG, Brazil.
FAU - Kratz, J M
AU  - Kratz JM
AD  - Laboratorio de Virologia Aplicada, Programa de Pos-graduacao em Farmacia, 
      Universidade Federal de Santa Catarina (UFSC), Florianopolis, SC, Brazil.
FAU - Simoes, C M O
AU  - Simoes CM
AD  - Laboratorio de Virologia Aplicada, Programa de Pos-graduacao em Farmacia, 
      Universidade Federal de Santa Catarina (UFSC), Florianopolis, SC, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20161103
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Antiviral Agents)
RN  - 0 (Biflavonoids)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Plant Extracts)
RN  - 0 (quercetin 3-O-methyl ether)
RN  - 0 (strychnobiflavone)
RN  - 9IKM0I5T1E (Quercetin)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/chemistry/pharmacology
MH  - Antiviral Agents/chemistry/*pharmacology
MH  - Biflavonoids/chemistry/*pharmacology
MH  - Brazil
MH  - Cell Line
MH  - Chemokine CCL2/metabolism
MH  - Chlorocebus aethiops
MH  - Cytokines/metabolism
MH  - Herpesvirus 1, Human/*drug effects/physiology
MH  - Humans
MH  - Plant Extracts/chemistry/pharmacology
MH  - Plants, Medicinal/chemistry
MH  - Quercetin/*analogs & derivatives/chemistry/pharmacology
MH  - Strychnos/*chemistry
MH  - Vero Cells
OTO - NOTNLM
OT  - Strychnos pseudoquina
OT  - HSV-1
OT  - HSV-2
OT  - quercetin 3-O-methyl ether
OT  - standardized extract
OT  - strychnobiflavone
EDAT- 2016/11/04 06:00
MHDA- 2017/03/23 06:00
CRDT- 2016/08/28 06:00
PHST- 2016/04/29 00:00 [received]
PHST- 2016/07/22 00:00 [revised]
PHST- 2016/08/22 00:00 [accepted]
PHST- 2016/11/04 06:00 [pubmed]
PHST- 2017/03/23 06:00 [medline]
PHST- 2016/08/28 06:00 [entrez]
AID - 10.1111/jam.13279 [doi]
PST - ppublish
SO  - J Appl Microbiol. 2016 Dec;121(6):1519-1529. doi: 10.1111/jam.13279. Epub 2016 
      Nov 3.