PMID- 2757007
OWN - NLM
STAT- MEDLINE
DCOM- 19890907
LR  - 20220408
IS  - 0361-8609 (Print)
IS  - 0361-8609 (Linking)
VI  - 32
IP  - 2
DP  - 1989 Oct
TI  - Effect of hydroxyurea on the rheological properties of sickle erythrocytes in 
      vivo.
PG  - 104-11
AB  - We have monitored the rheological effects of hydroxyurea (HU) on erythrocytes 
      obtained from two patients with severe sickle cell anemia who were enrolled in a 
      therapeutic trial of this drug. Erythrocyte membrane stability and whole cell and 
      membrane deformability of red cells from treated and untreated patients and 
      normal controls were determined in room air using an ektacytometer--a laser 
      viscodiffractometer. The percentage of dense cells was quantitated by 
      centrifugation on a discontinuous Stractan density gradient. F reticulocytes 
      (FR), absolute F reticulocytes (AFR), and F cells (FC) were determined by 
      single-cell radial immunolgic assays. After 1 year of treatment with HU, there 
      was a significant increase in the level of hemoglobin (Hb) F, FR, AFR, and FC. 
      The degree of anemia remained the same, but there was significant increase in the 
      mean cell volume (MCV) and a significant decrease in the mean corpuscular Hb 
      concentration (MCHC). Whole cell deformability improved by twofold, but membrane 
      stability remained within normal limits. The hydration status of sickle 
      erythrocytes improved as was indicated by a change toward normal in gradient 
      osmotic ektacytometry, an increase in RBC K+ content, a decrease in percent of 
      dense cells, and a decrease in the MCHC. The data indicate that, in addition to 
      its effect on the production of Hb, F, HU has a salutary effect on whole cell 
      deformability and on the hydration status of sickle erythrocytes. Determination 
      of the rheological properties of erythrocytes may be of value in monitoring the 
      response to HU.
FAU - Ballas, S K
AU  - Ballas SK
AD  - Cardeza Foundation for Hematologic Research, Philadelphia, PA 19107.
FAU - Dover, G J
AU  - Dover GJ
FAU - Charache, S
AU  - Charache S
LA  - eng
GR  - RR00035/RR/NCRR NIH HHS/United States
GR  - RR00722/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Am J Hematol
JT  - American journal of hematology
JID - 7610369
RN  - 9004-22-2 (Globins)
RN  - X6Q56QN5QC (Hydroxyurea)
SB  - IM
MH  - Anemia, Sickle Cell/blood/*drug therapy
MH  - Erythrocyte Deformability/*drug effects
MH  - Erythrocyte Indices
MH  - Erythrocyte Membrane/drug effects
MH  - Genotype
MH  - Globins/genetics
MH  - Humans
MH  - Hydroxyurea/*therapeutic use
EDAT- 1989/10/01 00:00
MHDA- 1989/10/01 00:01
CRDT- 1989/10/01 00:00
PHST- 1989/10/01 00:00 [pubmed]
PHST- 1989/10/01 00:01 [medline]
PHST- 1989/10/01 00:00 [entrez]
AID - 10.1002/ajh.2830320206 [doi]
PST - ppublish
SO  - Am J Hematol. 1989 Oct;32(2):104-11. doi: 10.1002/ajh.2830320206.