PMID- 27572051
OWN - NLM
STAT- MEDLINE
DCOM- 20170413
LR  - 20181113
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 14
IP  - 4
DP  - 2016 Oct
TI  - Notch 2 signaling contributes to cell growth, anti-apoptosis and metastasis in 
      laryngeal squamous cell carcinoma.
PG  - 3517-24
LID - 10.3892/mmr.2016.5688 [doi]
AB  - Notch signaling is important during the development of a variety of human tumors. 
      Depending on the context, Notch signaling can be either oncogenic or 
      anti‑proliferative, and therefore, its effects in cancer are unpredictable. The 
      aim of the present study was to identify the importance of Notch 2 in the cell 
      growth and metastasis of laryngeal squamous cell carcinoma (LSCC). The current 
      study performed quantum dots‑based immunofluorescence histochemistry to determine 
      expression of Notch 2 in 72 LSCC samples without lymph node metastasis, 23 LSCC 
      samples with lymph node metastasis and 31 samples from vocal cord polyps. It was 
      observed that Notch 2 was upregulated in LSCC tissue compared with normal vocal 
      cord polyps. This upregulation was further enhanced in LSCC tissues with lymph 
      node metastasis compared with LSCC tissues without lymph node metastasis. 
      Following knockdown of NOTCH2 expression in LSCC cells, the in vitro 
      tumorigenicity of Hep‑2 cells was inhibited, with growth, migration, invasion and 
      proliferation reduced, and apoptosis induced. Additionally, following 
      downregulation of Notch 2 protein expression, the protein expression levels of 
      phospho‑mitogen‑activated protein kinase 1 (p‑ERK), v‑myc avian myelocytomatosis 
      viral oncogene homolog and B‑cell CLL/lymphoma 2 (Bcl2) were also downregulated, 
      whereas, Bcl2‑associated X protein expression was upregulated. There were no 
      changes detected in the protein expression levels of total‑ERK, phospho‑v‑akt 
      murine thymoma viral oncogene homolog 1 (p‑Akt) and total‑Akt. The results of the 
      present study suggest that Notch 2 is important for the cell growth, 
      anti‑apoptosis and metastasis of LSCC. Therefore, Notch 2 inhibitors may have 
      therapeutic potential for the treatment of patients with LSCC via the inhibition 
      of cancer cell growth and metastasis.
FAU - Zou, You
AU  - Zou Y
AD  - Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan 
      University, Wuhan, Hubei 430060, P.R. China.
FAU - Fang, Fang
AU  - Fang F
AD  - Department of Medical Market, Renmin Hospital of Wuhan University, Wuhan, Hubei 
      430060, P.R. China.
FAU - Ding, Yong-Jun
AU  - Ding YJ
AD  - Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan 
      University, Wuhan, Hubei 430060, P.R. China.
FAU - Dai, Meng-Yuan
AU  - Dai MY
AD  - Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan 
      University, Wuhan, Hubei 430060, P.R. China.
FAU - Yi, Xing
AU  - Yi X
AD  - Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan 
      University, Wuhan, Hubei 430060, P.R. China.
FAU - Chen, Chen
AU  - Chen C
AD  - Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan 
      University, Wuhan, Hubei 430060, P.R. China.
FAU - Tao, Ze-Zhang
AU  - Tao ZZ
AD  - Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan 
      University, Wuhan, Hubei 430060, P.R. China.
FAU - Chen, Shi-Ming
AU  - Chen SM
AD  - Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan 
      University, Wuhan, Hubei 430060, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20160829
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (NOTCH2 protein, human)
RN  - 0 (Receptor, Notch2)
SB  - IM
MH  - *Apoptosis
MH  - Carcinoma, Squamous Cell/genetics/metabolism/*pathology
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - *Cell Proliferation
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/metabolism/*pathology
MH  - Larynx/metabolism/*pathology
MH  - Neoplasm Invasiveness/genetics/pathology
MH  - Neoplasm Metastasis/genetics/pathology
MH  - RNA Interference
MH  - Receptor, Notch2/genetics/*metabolism
MH  - *Signal Transduction
PMC - PMC5042778
EDAT- 2016/08/31 06:00
MHDA- 2017/04/14 06:00
PMCR- 2016/08/29
CRDT- 2016/08/31 06:00
PHST- 2015/06/18 00:00 [received]
PHST- 2016/05/31 00:00 [accepted]
PHST- 2016/08/31 06:00 [entrez]
PHST- 2016/08/31 06:00 [pubmed]
PHST- 2017/04/14 06:00 [medline]
PHST- 2016/08/29 00:00 [pmc-release]
AID - mmr-14-04-3517 [pii]
AID - 10.3892/mmr.2016.5688 [doi]
PST - ppublish
SO  - Mol Med Rep. 2016 Oct;14(4):3517-24. doi: 10.3892/mmr.2016.5688. Epub 2016 Aug 
      29.