PMID- 27572101
OWN - NLM
STAT- MEDLINE
DCOM- 20170303
LR  - 20170303
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 36
IP  - 4
DP  - 2016 Oct
TI  - Casticin induces DNA damage and inhibits DNA repair-associated protein expression 
      in B16F10 mouse melanoma cancer cells.
PG  - 2094-100
LID - 10.3892/or.2016.5027 [doi]
AB  - Casticin, a polymethoxyflavone, has been demonstrated to possess anticancer 
      activities, yet no study has shown in detail that casticin induces DNA damage in 
      lung cancer cells. The purpose of this study was to investigate the possible 
      molecular mechanisms of casticin which induce DNA damage and nuclear condensation 
      in murine melanoma cancer B16F10 cells. In this study, by examining and capturing 
      images using phase contrast microscopy, we found that casticin induced cell 
      morphological changes. Moreover, it decreased the total number of viable cells 
      which was measured by flow cytometry. Casticin-induced DNA damage and nuclear DNA 
      condensation were measured by DAPI staining, respectively. Western blotting 
      indicated that casticin decreased the protein levels of O6‑methylguanine-DNA 
      methyltransferase (MGMT), breast cancer 1, early onset (BRCA1), mediator of DNA 
      damage checkpoint 1 (MDC1), DNA-dependent protein kinase (DNA-PK) but increased 
      phospho-p53 tumor suppressor protein (p-p53), phospho-ataxia telangiectasia 
      mutated kinase (p-ATM), phospho-histone H2A.X (Ser139) and poly(ADP-ribose) 
      polymerase (PARP) in the B16F10 cells. Furthermore, we used confocal laser system 
      microscopy to examine the protein expression levels and we found that casticin 
      increased the expression of p-p53 and p-H2A.X in the B16F10 cells. Collectively, 
      casticin induced DNA damage and affected DNA repair proteins in the B16F10 cells 
      in vitro.
FAU - Shih, Yung-Luen
AU  - Shih YL
AD  - Department of Pathology and Laboratory Medicine, Shin Kong Wu Ho-Su Memorial 
      Hospital, Taipei, Taiwan, R.O.C.
FAU - Chou, Jason
AU  - Chou J
AD  - Department of Pathology, Cheng-Hsin General Hospital, Taipei, Taiwan, R.O.C.
FAU - Yeh, Ming-Yang
AU  - Yeh MY
AD  - Office of Director, Cheng-Hsin General Hospital, Taipei, Taiwan, R.O.C.
FAU - Chou, Hsiao-Min
AU  - Chou HM
AD  - Department of Pathology and Laboratory Medicine, Shin Kong Wu Ho-Su Memorial 
      Hospital, Taipei, Taiwan, R.O.C.
FAU - Chou, Hsiu-Chen
AU  - Chou HC
AD  - Department of Pathology and Laboratory Medicine, Shin Kong Wu Ho-Su Memorial 
      Hospital, Taipei, Taiwan, R.O.C.
FAU - Lu, Hsu-Feng
AU  - Lu HF
AD  - Department of Restaurant, Hotel and Institutional Management, Fu-Jen Catholic 
      University, New Taipei, Taiwan, R.O.C.
FAU - Shang, Hung-Sheng
AU  - Shang HS
AD  - Department of Pathology, National Defense Medical Center, Division of Clinical 
      Pathology, Tri-Service General Hospital, Taipei, Taiwan, R.O.C.
FAU - Chueh, Fu-Shin
AU  - Chueh FS
AD  - Department of Health and Nutrition Biotechnology, Asia University, Taichung, 
      Taiwan, R.O.C.
FAU - Chu, Yung-Lin
AU  - Chu YL
AD  - International Master's Degree Program in Food Science, International College, 
      National Pingtung University of Science and Technology, Pingtung, Taiwan, R.O.C.
FAU - Hsueh, Shu-Ching
AU  - Hsueh SC
AD  - Department of Clinical Pathology, Cheng-Hsin General Hospital, Taipei, Taiwan, 
      R.O.C.
FAU - Chung, Jing-Gung
AU  - Chung JG
AD  - Department of Biological Science and Technology, China Medical University, 
      Taichung, Taiwan, R.O.C.
LA  - eng
PT  - Journal Article
DEP - 20160817
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (Antineoplastic Agents, Phytogenic)
RN  - 0 (Flavonoids)
RN  - 753GT729OU (casticin)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents, Phytogenic/*pharmacology
MH  - Blotting, Western
MH  - Cell Line, Tumor
MH  - DNA Damage/*drug effects
MH  - DNA Repair/*drug effects
MH  - Disease Models, Animal
MH  - Flavonoids/*pharmacology
MH  - Flow Cytometry
MH  - *Melanoma
MH  - Mice
MH  - Microscopy, Confocal
MH  - Microscopy, Phase-Contrast
EDAT- 2016/08/31 06:00
MHDA- 2017/03/04 06:00
CRDT- 2016/08/31 06:00
PHST- 2016/02/01 00:00 [received]
PHST- 2016/03/09 00:00 [accepted]
PHST- 2016/08/31 06:00 [entrez]
PHST- 2016/08/31 06:00 [pubmed]
PHST- 2017/03/04 06:00 [medline]
AID - 10.3892/or.2016.5027 [doi]
PST - ppublish
SO  - Oncol Rep. 2016 Oct;36(4):2094-100. doi: 10.3892/or.2016.5027. Epub 2016 Aug 17.