PMID- 27572674
OWN - NLM
STAT- MEDLINE
DCOM- 20170405
LR  - 20211204
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 14
IP  - 4
DP  - 2016 Oct
TI  - Effect of autophagy induced by dexamethasone on senescence in chondrocytes.
PG  - 3037-44
LID - 10.3892/mmr.2016.5662 [doi]
AB  - The aim of the current study was to explore the effects of dexamethasone (DXM) on 
      autophagy and senescence in chondrocytes. Collagen II and aggrecan were examined 
      in normal chondrocytes isolated from Sprague‑Dawley rats. Following stimulation 
      with DXM, LysoTracker Red staining, monodansylcadaverine (MDC) staining, green 
      fluorescent protein‑red fluorescent protein‑light chain 3 (LC3) and western 
      blotting were used to detect autophagy levels in the chondrocytes. Mechanistic 
      target of rapamycin (mTOR) pathway‑associated molecules were investigated by 
      western blotting. Cell senescence was analyzed by senescence‑associated 
      (SA)‑beta‑galactosidase (beta‑gal) staining. A dose‑dependent increase in the number of 
      autophagic vacuoles was observed in the DXM‑treated chondrocytes, as demonstrated 
      by LysoTracker Red and MDC staining. A dose‑dependent increase in autophagosome 
      formation was observed in the DXM‑treated chondrocytes. Expression of LC3‑II and 
      beclin‑1 was increased by DXM, in particular in the cells treated with DXM for 
      4 days. However, P62 expression was reduced as a result of treatment. SA‑beta‑gal 
      staining indicated that DXM increased cell senescence. Notably, DXM‑induced cell 
      senescence was exacerbated by the autophagic inhibitor 3‑MA. Autophagy induced by 
      DXM protected chondrocytes from senescence, and it is suggested that the mTOR 
      pathway may be involved in the activation of DXM‑induced autophagy.
FAU - Xue, Enxing
AU  - Xue E
AD  - Department of Orthopedic Surgery, Nanfang Hospital, Southern Medical University, 
      Guangzhou, Guangdong 510515, P.R. China.
FAU - Zhang, Yu
AU  - Zhang Y
AD  - Department of Orthopedic Surgery, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. 
      China.
FAU - Song, Bing
AU  - Song B
AD  - Department of Orthopedic Surgery, Nanfang Hospital, Southern Medical University, 
      Guangzhou, Guangdong 510515, P.R. China.
FAU - Xiao, Jun
AU  - Xiao J
AD  - Department of Orthopedic Surgery, Nanfang Hospital, Southern Medical University, 
      Guangzhou, Guangdong 510515, P.R. China.
FAU - Shi, Zhanjun
AU  - Shi Z
AD  - Department of Orthopedic Surgery, Nanfang Hospital, Southern Medical University, 
      Guangzhou, Guangdong 510515, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20160822
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Anti-Inflammatory Agents)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Autophagy/*drug effects
MH  - Cells, Cultured
MH  - Cellular Senescence/*drug effects
MH  - Chondrocytes/cytology/*drug effects/metabolism
MH  - Dexamethasone/*pharmacology
MH  - Male
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/drug effects
MH  - TOR Serine-Threonine Kinases/antagonists & inhibitors/metabolism
PMC - PMC5042789
EDAT- 2016/08/31 06:00
MHDA- 2017/04/06 06:00
PMCR- 2016/08/22
CRDT- 2016/08/31 06:00
PHST- 2015/08/03 00:00 [received]
PHST- 2016/08/08 00:00 [accepted]
PHST- 2016/08/31 06:00 [entrez]
PHST- 2016/08/31 06:00 [pubmed]
PHST- 2017/04/06 06:00 [medline]
PHST- 2016/08/22 00:00 [pmc-release]
AID - mmr-14-04-3037 [pii]
AID - 10.3892/mmr.2016.5662 [doi]
PST - ppublish
SO  - Mol Med Rep. 2016 Oct;14(4):3037-44. doi: 10.3892/mmr.2016.5662. Epub 2016 Aug 
      22.