PMID- 27573980
OWN - NLM
STAT- MEDLINE
DCOM- 20180319
LR  - 20221207
IS  - 1753-0407 (Electronic)
IS  - 1753-0407 (Linking)
VI  - 9
IP  - 7
DP  - 2017 Jul
TI  - DNA methylation of tumor necrosis factor-alpha, monocyte chemoattractant protein-1, 
      and adiponectin genes in visceral adipose tissue is related to type 2 diabetes in 
      the Xinjiang Uygur population.
PG  - 699-706
LID - 10.1111/1753-0407.12478 [doi]
AB  - BACKGROUND: The higher probability of type 2 diabetes mellitus (T2DM) in the 
      Uygur population is due to a greater waist:  hip ratio and visceral fat. This 
      study investigated DNA methylation of tumor necrosis factor-alpha (TNF), monocyte 
      chemoattractant protein-1 (MCP1), and adiponectin (ADIPOQ) in visceral adipose 
      tissue in T2DM. METHODS: Visceral adipose tissue was collected from Uygur 
      individuals and divided into normal control (NC; n = 50), obese (Ob; n = 48), and 
      T2DM (n = 26) groups. Expression of TNF, ADIPOQ, and MCP1 mRNA and DNA 
      methylation status were quantified by reverse transcription-polymerase chain 
      reaction and denaturing HPLC. RESULTS: The respective methylation-positive rate 
      for ADIPOQ increased gradually from the NC to Ob to T2DM groups (34.0 %, 47.9 %, 
      and 65.4 %; P < 0.05), decreased gradually for TNF (70.0 %, 47.9 %, and 26.9 %; 
      P < 0.01), and did not differ significantly for MCP1 (0 %, 2.08 %, and 0 %). 
      Compared with the NC group, ADIPOQ mRNA expression was significantly lower in the 
      Ob and T2DM groups (median 0.7162 vs 0.4244 and 0.4093, respectively; P < 0.05), 
      whereas TNF and MCP1 expression was significantly higher (median TNF expression: 
      0.0250 vs 0.1096 and 0.0734 respectively; median MCP1 expression 0.1588 vs 0.1937 
      and 0.1983, respectively; P < 0.05 for all). Expression of ADIPOQ and TNF was 
      significantly lower in methylation-negative (median 0.7870 and 0.1988, 
      respectively) than methylation-positive (median 0.2700 and 0.0542, respectively) 
      groups (P < 0.01). CONCLUSIONS: Lower ADIPOQ and higher TNF and MCP1 mRNA 
      expression in visceral adipose tissue may be correlated with obesity and T2DM in 
      the Uygur population. Promoter DNA methylation affects expression of ADIPOQ and 
      TNF.
CI  - (c) 2016 Ruijin Hospital, Shanghai Jiaotong University School of Medicine and John 
      Wiley & Sons Australia, Ltd.
FAU - Zhang, Jun
AU  - Zhang J
AD  - Shihezi University School of Medicine, Shihezi, China.
FAU - Wang, Cuizhe
AU  - Wang C
AD  - Shihezi University School of Medicine, Shihezi, China.
FAU - Ha, Xiaodan
AU  - Ha X
AD  - Shihezi University School of Medicine, Shihezi, China.
FAU - Li, Wei
AU  - Li W
AD  - Shihezi University School of Medicine, Shihezi, China.
AD  - Shihezi University School of Medicine in the First Affiliated Hospital Clinical 
      Laboratory, Shihezi, China.
FAU - Xu, Peng
AU  - Xu P
AD  - Shihezi University School of Medicine, Shihezi, China.
FAU - Gu, Yajuan
AU  - Gu Y
AD  - Shihezi University School of Medicine, Shihezi, China.
FAU - Wang, Tingting
AU  - Wang T
AD  - Shihezi University School of Medicine, Shihezi, China.
FAU - Wang, Yan
AU  - Wang Y
AD  - Endocrinology Department of Xinjiang Uygur Autonomous Region People's Hospital, 
      Urumqi, China.
FAU - Xie, Jianxin
AU  - Xie J
AD  - Shihezi University School of Medicine, Shihezi, China.
LA  - eng
PT  - Journal Article
DEP - 20161108
PL  - Australia
TA  - J Diabetes
JT  - Journal of diabetes
JID - 101504326
RN  - 0 (ADIPOQ protein, human)
RN  - 0 (Adiponectin)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adiponectin/*genetics
MH  - Adult
MH  - Aged
MH  - Asian People/genetics
MH  - Chemokine CCL2/*genetics
MH  - China
MH  - *DNA Methylation
MH  - Diabetes Mellitus, Type 2/ethnology/*genetics
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Intra-Abdominal Fat/*metabolism
MH  - Logistic Models
MH  - Male
MH  - Middle Aged
MH  - Obesity/ethnology/genetics
MH  - Tumor Necrosis Factor-alpha/*genetics
OTO - NOTNLM
OT  - DNA methylation
OT  - DNA甲基化
OT  - Uygur population
OT  - adiponectin (ADIPOQ)
OT  - monocyte chemoattractant protein-1 (MCP1)
OT  - tumor necrosis factor-alpha (TNF)
OT  - 单核细胞趋化因子-1(MCP1)
OT  - 维吾尔族人群
OT  - 肿瘤坏死因子-alpha(TNF)
OT  - 脂联素(ADIPOQ)
EDAT- 2016/08/31 06:00
MHDA- 2018/03/20 06:00
CRDT- 2016/08/31 06:00
PHST- 2016/03/07 00:00 [received]
PHST- 2016/08/12 00:00 [revised]
PHST- 2016/08/28 00:00 [accepted]
PHST- 2016/08/31 06:00 [pubmed]
PHST- 2018/03/20 06:00 [medline]
PHST- 2016/08/31 06:00 [entrez]
AID - 10.1111/1753-0407.12478 [doi]
PST - ppublish
SO  - J Diabetes. 2017 Jul;9(7):699-706. doi: 10.1111/1753-0407.12478. Epub 2016 Nov 8.