PMID- 27574106
OWN - NLM
STAT- MEDLINE
DCOM- 20170403
LR  - 20220113
IS  - 1791-2423 (Electronic)
IS  - 1019-6439 (Linking)
VI  - 49
IP  - 3
DP  - 2016 Sep
TI  - Long non-coding RNA HOTAIR modulates HLA-G expression by absorbing miR-148a in 
      human cervical cancer.
PG  - 943-52
LID - 10.3892/ijo.2016.3589 [doi]
AB  - The long non-coding RNA HOX transcript antisense RNA (HOTAIR) has been found 
      overexpressed in many human malignancies and involved in tumor progression and 
      metastasis. However, little is known about the potential biological roles of 
      HOTAIR in tumor escape. In the present study, the expression of HOTAIR was 
      detected in 59 paired cervical cancer tissue samples by real-time PCR and then 
      subjected to correlation analysis with clinical features. The effects of HOTAIR 
      on cervical cancer cells as well as the expression of human leukocyte antigen 
      (HLA)-G were studied by overexpression and RNA interference approaches. Insight 
      into the mechanism of HOTAIR acting as competitive endogenous RNAs (ceRNAs) was 
      gained from bioinformatic analysis and luciferase assays. HOTAIR expression was 
      obviously increased in cervical cancer tissue. HOTAIR upregulation was associated 
      with advanced pathological stage, histology, lymph node invasion and lymphatic 
      metastasis, and also correlated with shorter overall survival of cervical cancer 
      patients. Furthermore, HOTAIR overexpression promoted the proliferation, 
      migration and invasion of cervical cancer cells, while HOTAIR knockdown inhibited 
      cell invasion and cell viability, induced apoptosis and inhibited growth in vitro 
      and in vivo. Moreover, HOTAIR modulated human leucocyte antigen-G (HLA-G) 
      expression by competitively binding miR-148a. Our data suggest that HOTAIR plays 
      an important oncogenic role in cervical cancer and might serve as a marker for 
      cervical cancer prognosis and a potential target for therapeutic intervention.
FAU - Sun, Jinbao
AU  - Sun J
AD  - Department of Gynecological Ward, People's Hospital, Cangzhou, Hebei 061000, P.R. 
      China.
FAU - Chu, Haipeng
AU  - Chu H
AD  - Department of Obstetrics-Gynecology, Daqing LongNan Hospital, Daqing, 
      Heilongjiang 163001, P.R. China.
FAU - Ji, Jianghai
AU  - Ji J
AD  - Department of Gynecological Ward, People's Hospital, Cangzhou, Hebei 061000, P.R. 
      China.
FAU - Huo, Gaoxiang
AU  - Huo G
AD  - Department of Gynecological Ward, People's Hospital, Cangzhou, Hebei 061000, P.R. 
      China.
FAU - Song, Qinglei
AU  - Song Q
AD  - Department of Gynecological Ward, People's Hospital, Cangzhou, Hebei 061000, P.R. 
      China.
FAU - Zhang, Xue
AU  - Zhang X
AD  - Department of Gynecological Ward, People's Hospital, Cangzhou, Hebei 061000, P.R. 
      China.
LA  - eng
PT  - Journal Article
DEP - 20160630
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (HLA-G Antigens)
RN  - 0 (HOTAIR long untranslated RNA, human)
RN  - 0 (MIRN148 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - HLA-G Antigens/*genetics
MH  - HeLa Cells
MH  - Humans
MH  - Mice
MH  - MicroRNAs/*genetics
MH  - Neoplasm Transplantation
MH  - RNA, Long Noncoding/*genetics
MH  - Uterine Cervical Neoplasms/*genetics
EDAT- 2016/08/31 06:00
MHDA- 2017/04/04 06:00
CRDT- 2016/08/31 06:00
PHST- 2015/12/02 00:00 [received]
PHST- 2016/01/21 00:00 [accepted]
PHST- 2016/08/31 06:00 [entrez]
PHST- 2016/08/31 06:00 [pubmed]
PHST- 2017/04/04 06:00 [medline]
AID - 10.3892/ijo.2016.3589 [doi]
PST - ppublish
SO  - Int J Oncol. 2016 Sep;49(3):943-52. doi: 10.3892/ijo.2016.3589. Epub 2016 Jun 30.