PMID- 27574677
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160830
LR  - 20240325
IS  - 2321-3868 (Print)
IS  - 2321-3876 (Electronic)
IS  - 2321-3868 (Linking)
VI  - 4
DP  - 2016
TI  - Evaluation of the wound healing potential of isoquercetin-based cream on scald 
      burn injury in rats.
PG  - 7
LID - 10.1186/s41038-016-0032-1 [doi]
LID - 7
AB  - BACKGROUND: The present study was designed to evaluate the potential of 
      isoquercetin-based cream formulation on scald burn wound injury in rats. METHODS: 
      Four isoquercetin-based cream formulations viz. 0.01, 0.02, 0.04, and 0.06 % w/w 
      were prepared. Cream base and standard anti-burn cream containing silver 
      sulfadiazine were also used for comparison. Scald burn was given to rats by 
      pouring water at 90  degrees C on a shaved dorsal area of 20 mm(2). Deep second-degree 
      burn injury was produced which was evaluated for the next 21 days for the 
      percentage of wound contraction and period of epithelialization. On day 21, the 
      rats were sacrificed and histopathological slides were prepared using 
      hematoxylin-eosin staining. Burned tissue was also screened for levels of 
      oxidative stress using thiobarbituric acid reactive species (TBARS) and reduced 
      glutathione (GSH) estimation. RESULTS: There was a significant increase in the 
      percentage of wound contraction and a significant decrease in the period of 
      epithelialization in isoquercetin-based cream-treated groups as compared with the 
      control group. However, most significant results were obtained with isoquercetin 
      0.06 % w/w cream. Histologically, isoquercetin 0.06 % w/w cream treatment 
      resulted in almost complete re-epithelialization and re-structuring of the wound 
      tissue. There was a significant rise in TBARS and a decrease in GSH levels in the 
      burn injury group which was reversed to a major extent by the application of 
      isoquercetin-based cream. CONCLUSIONS: The results indicate the wound healing 
      potential of isoquercetin-based cream. Tissue biochemical studies indicate 
      towards a possible role of free radical scavenging in the observed effects of 
      isoquercetin in wound healing.
FAU - Bhatia, Nitish
AU  - Bhatia N
AD  - Pharmacology Research Laboratory, Department of Pharmacology, Khalsa College of 
      Pharmacy, Amritsar, India.
FAU - Kaur, Gursharan
AU  - Kaur G
AD  - Pharmacology Research Laboratory, Department of Pharmacology, Khalsa College of 
      Pharmacy, Amritsar, India.
FAU - Soni, Varinder
AU  - Soni V
AD  - Department of Pharmaceutical Analysis, Khalsa College of Pharmacy, Amritsar, 
      India.
FAU - Kataria, Juhi
AU  - Kataria J
AD  - Department of Biochemistry, Khalsa College of Pharmacy and Technology, Amritsar, 
      India.
FAU - Dhawan, Ravi K
AU  - Dhawan RK
AD  - Pharmacology Research Laboratory, Department of Pharmacology, Khalsa College of 
      Pharmacy, Amritsar, India.
LA  - eng
PT  - Journal Article
DEP - 20160316
PL  - England
TA  - Burns Trauma
JT  - Burns & trauma
JID - 101651457
PMC - PMC4964293
OTO - NOTNLM
OT  - Burn
OT  - Free radicals
OT  - Isoquercetin
OT  - Scald
OT  - Wound healing
EDAT- 2016/08/31 06:00
MHDA- 2016/08/31 06:01
PMCR- 2016/03/16
CRDT- 2016/08/31 06:00
PHST- 2015/07/11 00:00 [received]
PHST- 2016/01/31 00:00 [accepted]
PHST- 2016/08/31 06:00 [entrez]
PHST- 2016/08/31 06:00 [pubmed]
PHST- 2016/08/31 06:01 [medline]
PHST- 2016/03/16 00:00 [pmc-release]
AID - 32 [pii]
AID - 10.1186/s41038-016-0032-1 [doi]
PST - epublish
SO  - Burns Trauma. 2016 Mar 16;4:7. doi: 10.1186/s41038-016-0032-1. eCollection 2016.