PMID- 27574922
OWN - NLM
STAT- MEDLINE
DCOM- 20180927
LR  - 20220317
IS  - 1536-3686 (Electronic)
IS  - 1075-2765 (Linking)
VI  - 25
IP  - 3
DP  - 2018 May/Jun
TI  - Effect of Electroacupuncture in "Zusanli" and "Kunlun" Acupoints on TLR4 
      Signaling Pathway of Adjuvant Arthritis Rats.
PG  - e314-e319
LID - 10.1097/MJT.0000000000000477 [doi]
AB  - BACKGROUND: Rheumatoid arthritis (RA) is a chronic systemic autoimmune disease. 
      Previous study suggested that toll-like receptor (TLR) signaling pathway 
      contributes to the development and progression of RA. In recent years, 
      acupuncture has become one of the most vital treatments of arthralgia. But little 
      is known about the mechanisms of improving RA by acupuncture. STUDY QUESTION: The 
      study studied the effect of electroacupuncture in "Zusanli" and "Kunlun" 
      acupoints on the expression of TLR4, myeloid differentiation factor 88 (MYD88), 
      and NF-kappaB in adjuvant arthritis rats to clarify the molecular mechanism of 
      acupuncture of RA. STUDY DESIGN: A rat model of adjuvant arthritis was 
      established with injection of 0.1 mL Freund complete adjuvant in the right 
      hindlimb footpad. We next punctured the Zusanli and Kunlun acupoints with 0.25 x 
      40-mm acupuncture needles to 5-mm depth. Then, we performed electroacupuncture 
      treatment for 28 days with frequency of 2 Hz and intensity of 2 mA, once a day 
      and 30 minutes each time. MEASURES AND OUTCOMES: Arthritis index and paw swelling 
      were measured every week. FQ-PCR and western blot were used to detect the 
      expression of TLR4, MYD88, and NF-kappaB. RESULTS: Paw swelling of rats injected with 
      Freund complete adjuvant was more serious than that of the normal rats, which 
      illustrated the successful establishment of adjuvant arthritis rat model. After 
      treatment for 14 days, the paw swelling and joint symptoms score decreased, paw 
      tissue inflammation eased in the rats of treatment group compared with the model 
      group during the same period. After treatment for 28 days, the expression of 
      TLR4, MYD88, and NF-kappaB in the ankle bone tissues decreased at both mRNA and 
      protein levels. CONCLUSIONS: Stimulation with electric needle in Zusanli and 
      Kunlun acupoints can reduce the expression of TLR4, MYD88, and NF-kappaB, which play 
      an important role in treatment of adjuvant arthritis.
FAU - Dong, Zhe-Qing
AU  - Dong ZQ
AD  - Clinical Laboratory, The Third Affiliated Hospital of Zhejiang Chinese Medical 
      University, Hangzhou, China.
FAU - Zhu, Ji
AU  - Zhu J
AD  - Clinical Laboratory, The Third Affiliated Hospital of Zhejiang Chinese Medical 
      University, Hangzhou, China.
FAU - Lu, De-Zhao
AU  - Lu DZ
AD  - College of Life Science, Zhejiang Chinese Medical University, Hangzhou, China.
FAU - Chen, Qin
AU  - Chen Q
AD  - Clinical Laboratory, The Third Affiliated Hospital of Zhejiang Chinese Medical 
      University, Hangzhou, China.
FAU - Xu, Ying-Ling
AU  - Xu YL
AD  - College of Life Science, Zhejiang Chinese Medical University, Hangzhou, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Ther
JT  - American journal of therapeutics
JID - 9441347
RN  - 0 (NF-kappa B)
RN  - 0 (Tlr4 protein, rat)
RN  - 0 (Toll-Like Receptor 4)
RN  - 9007-81-2 (Freund's Adjuvant)
SB  - IM
MH  - *Acupuncture Points
MH  - Animals
MH  - Arthritis, Experimental/immunology/*therapy
MH  - Arthritis, Rheumatoid/immunology/*therapy
MH  - Disease Models, Animal
MH  - Electroacupuncture/*methods
MH  - Freund's Adjuvant/immunology
MH  - Humans
MH  - Male
MH  - NF-kappa B
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/immunology
MH  - Toll-Like Receptor 4/*metabolism
MH  - Treatment Outcome
EDAT- 2016/08/31 06:00
MHDA- 2018/09/28 06:00
CRDT- 2016/08/31 06:00
PHST- 2016/08/31 06:00 [pubmed]
PHST- 2018/09/28 06:00 [medline]
PHST- 2016/08/31 06:00 [entrez]
AID - 10.1097/MJT.0000000000000477 [doi]
PST - ppublish
SO  - Am J Ther. 2018 May/Jun;25(3):e314-e319. doi: 10.1097/MJT.0000000000000477.