PMID- 27576943
OWN - NLM
STAT- MEDLINE
DCOM- 20190627
LR  - 20191210
IS  - 1365-2818 (Electronic)
IS  - 0022-2720 (Linking)
VI  - 265
IP  - 1
DP  - 2017 Jan
TI  - Cytogenetic profiling by FISH microscopy and comparison with light microscopy and 
      complete blood count findings in myelodysplastic syndrome.
PG  - 94-100
LID - 10.1111/jmi.12467 [doi]
AB  - Karyotype, bone marrow blast percentage and cytopenia influence the prognosis of 
      myelodysplastic syndrome. We studied the abnormalities detected by fluorescence 
      in situ hybridization (FISH) in myelodysplastic syndrome and associated 
      haematological profile with abnormalities detected by FISH. Complete blood 
      counts, peripheral blood and bone marrow of patients were evaluated for 
      cytopenia, dysplasia and blasts. FISH probes were used to detect del(5q), gain of 
      chromosome 8, de (7q/-7) and del(20 q). Multiple regression analysis was used to 
      study the association of FISH abnormalities, age and sex with haematological 
      profile. Mc Nemar's test studied the relationship between FISH abnormalities and 
      dysplastic features in bone marrow. Cytogenetic abnormalities were detected by 
      FISH in 25.7% of patients. Del(20 q) was seen in 14.2% of patients. FISH was able 
      to predict changes in peripheral blood blast count by 80% (p < 0.0001). 
      Cytogenetic abnormalities were not seen in 74.2% of patients. Groups with FISH 
      abnormalities have a different haematological profile, and these abnormalities 
      have a significant effect on blast percentage.
CI  - (c) 2016 The Authors Journal of Microscopy (c) 2016 Royal Microscopical Society.
FAU - Chandrashekar, V
AU  - Chandrashekar V
AD  - Department of Hematology Apollo Hospitals, 21, off Greams Road, Greams Lane 
      Chennai - 600006.
FAU - Tarigopula, A
AU  - Tarigopula A
AD  - Department of Centralised Molecular Diagnostics Apollo Hospitals, 21, off Greams 
      Road, Greams Lane Chennai - 600006.
FAU - Govindasami, P
AU  - Govindasami P
AD  - Perumal G M.Sc, PG DMLT, (P.hD) Department of Centralised Molecular Diagnostics 
      Apollo Hospitals, 21, off Greams Road, India, Greams Lane Chennai - 600006.
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
DEP - 20160831
PL  - England
TA  - J Microsc
JT  - Journal of microscopy
JID - 0204522
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Blood Cell Count
MH  - Cytogenetics/*methods
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Microscopy/*methods
MH  - Microscopy, Fluorescence/*methods
MH  - Middle Aged
MH  - Myelodysplastic Syndromes/*pathology
OTO - NOTNLM
OT  - Fluorescence in situ hybridization
OT  - multiple regression
OT  - myelodysplastic syndrome
EDAT- 2016/09/01 06:00
MHDA- 2019/06/30 06:00
CRDT- 2016/09/01 06:00
PHST- 2016/04/05 00:00 [received]
PHST- 2016/07/10 00:00 [revised]
PHST- 2016/08/02 00:00 [accepted]
PHST- 2016/09/01 06:00 [pubmed]
PHST- 2019/06/30 06:00 [medline]
PHST- 2016/09/01 06:00 [entrez]
AID - 10.1111/jmi.12467 [doi]
PST - ppublish
SO  - J Microsc. 2017 Jan;265(1):94-100. doi: 10.1111/jmi.12467. Epub 2016 Aug 31.