PMID- 27579858
OWN - NLM
STAT- MEDLINE
DCOM- 20171107
LR  - 20230203
IS  - 1935-3456 (Electronic)
IS  - 1933-0219 (Print)
IS  - 1933-0219 (Linking)
VI  - 10
IP  - 2
DP  - 2017 Mar
TI  - Dendritic cells from the human female reproductive tract rapidly capture and 
      respond to HIV.
PG  - 531-544
LID - 10.1038/mi.2016.72 [doi]
AB  - Dendritic cells (DCs) throughout the female reproductive tract (FRT) were 
      examined for phenotype, HIV capture ability and innate anti-HIV responses. Two 
      main CD11c(+) DC subsets were identified: CD11b(+) and CD11b(low) DCs. 
      CD11b(+)CD14(+) DCs were the most abundant throughout the tract. A majority of 
      CD11c(+)CD14(+) cells corresponded to CD1c(+) myeloid DCs, whereas the rest 
      lacked CD1c and CD163 expression (macrophage marker) and may represent 
      monocyte-derived cells. In addition, we identified CD103(+) DCs, located 
      exclusively in the endometrium, whereas DC-SIGN(+) DCs were broadly distributed 
      throughout the FRT. Following exposure to GFP-labeled HIV particles, CD14(+) 
      DC-SIGN(+) as well as CD14(+) DC-SIGN(-) cells captured virus, with  approximately 30% of these 
      cells representing CD1c(+) myeloid DCs. CD103(+) DCs lacked HIV capture ability. 
      Exposure of FRT DCs to HIV induced secretion of CCL2, CCR5 ligands, interleukin 
      (IL)-8, elafin, and secretory leukocyte peptidase inhibitor (SLPI) within 3 h of 
      exposure, whereas classical pro-inflammatory molecules did not change and 
      interferon-alpha2 and IL-10 were undetectable. Furthermore, elafin and SLPI 
      upregulation, but not CCL5, were suppressed by estradiol pre-treatment. Our 
      results suggest that specific DC subsets in the FRT have the potential for 
      capture and dissemination of HIV, exert antiviral responses and likely contribute 
      to the recruitment of HIV-target cells through the secretion of innate immune 
      molecules.
FAU - Rodriguez-Garcia, M
AU  - Rodriguez-Garcia M
AD  - Department of Physiology and Neurobiology, Geisel School of Medicine at 
      Dartmouth, Lebanon, New Hampshire, USA.
FAU - Shen, Z
AU  - Shen Z
AD  - Department of Physiology and Neurobiology, Geisel School of Medicine at 
      Dartmouth, Lebanon, New Hampshire, USA.
FAU - Barr, F D
AU  - Barr FD
AD  - Department of Physiology and Neurobiology, Geisel School of Medicine at 
      Dartmouth, Lebanon, New Hampshire, USA.
FAU - Boesch, A W
AU  - Boesch AW
AD  - Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire, USA.
FAU - Ackerman, M E
AU  - Ackerman ME
AD  - Thayer School of Engineering, Dartmouth College, Hanover, New Hampshire, USA.
FAU - Kappes, J C
AU  - Kappes JC
AD  - Department of Medicine and UAB Center for AIDS Research, University of Alabama at 
      Birmingham, Research Service Birmingham, Birmingham, Alabama, USA.
AD  - Birmingham Veterans Affairs Medical Center, Research Service Birmingham, 
      Birmingham, Alabama, USA.
FAU - Ochsenbauer, C
AU  - Ochsenbauer C
AD  - Department of Medicine and UAB Center for AIDS Research, University of Alabama at 
      Birmingham, Research Service Birmingham, Birmingham, Alabama, USA.
FAU - Wira, C R
AU  - Wira CR
AD  - Department of Physiology and Neurobiology, Geisel School of Medicine at 
      Dartmouth, Lebanon, New Hampshire, USA.
LA  - eng
GR  - T32 CA073479/CA/NCI NIH HHS/United States
GR  - P30 AI027767/AI/NIAID NIH HHS/United States
GR  - R01 AI102838/AI/NIAID NIH HHS/United States
GR  - P30 CA023108/CA/NCI NIH HHS/United States
GR  - R01 AI117739/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20160831
PL  - United States
TA  - Mucosal Immunol
JT  - Mucosal immunology
JID - 101299742
RN  - 0 (CCR5 protein, human)
RN  - 0 (CD11c Antigen)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Chemokine CCL2)
RN  - 0 (DC-specific ICAM-3 grabbing nonintegrin)
RN  - 0 (Elafin)
RN  - 0 (Interleukin-8)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Receptors, CCR5)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (SLPI protein, human)
RN  - 0 (Secretory Leukocyte Peptidase Inhibitor)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - CD11c Antigen/metabolism
MH  - Cell Adhesion Molecules/metabolism
MH  - Cells, Cultured
MH  - Chemokine CCL2/metabolism
MH  - Dendritic Cells/*immunology/virology
MH  - Elafin/metabolism
MH  - Estradiol/pharmacology
MH  - Female
MH  - Genitalia, Female/*immunology
MH  - HIV/*immunology/pathogenicity
MH  - HIV Infections/*immunology/transmission
MH  - Humans
MH  - *Immunity, Innate
MH  - Interleukin-8/metabolism
MH  - Lectins, C-Type/metabolism
MH  - Lipopolysaccharide Receptors/metabolism
MH  - Phagocytosis
MH  - Receptors, CCR5/metabolism
MH  - Receptors, Cell Surface/metabolism
MH  - Secretory Leukocyte Peptidase Inhibitor/metabolism
PMC - PMC5332537
MID - NIHMS805792
COIS- Disclosure. The authors declared no conflict of interest.
EDAT- 2016/09/01 06:00
MHDA- 2017/11/08 06:00
PMCR- 2017/03/06
CRDT- 2016/09/01 06:00
PHST- 2016/01/07 00:00 [received]
PHST- 2016/07/07 00:00 [accepted]
PHST- 2016/09/01 06:00 [pubmed]
PHST- 2017/11/08 06:00 [medline]
PHST- 2016/09/01 06:00 [entrez]
PHST- 2017/03/06 00:00 [pmc-release]
AID - S1933-0219(22)00664-X [pii]
AID - 10.1038/mi.2016.72 [doi]
PST - ppublish
SO  - Mucosal Immunol. 2017 Mar;10(2):531-544. doi: 10.1038/mi.2016.72. Epub 2016 Aug 
      31.