PMID- 27580160
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20180718
LR  - 20180718
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Linking)
VI  - 8
IP  - 37
DP  - 2016 Sep 21
TI  - Construction of Thermophilic Lipase-Embedded Metal-Organic Frameworks via 
      Biomimetic Mineralization: A Biocatalyst for Ester Hydrolysis and Kinetic 
      Resolution.
PG  - 24517-24
LID - 10.1021/acsami.6b05538 [doi]
AB  - Enhancing the activity and stability of enzymes and improving their reusability 
      are critical challenges in the field of enzyme immobilization. Here we report a 
      facile and efficient biomimetic mineralization to embed thermophilic lipase QLM 
      in zeolite imidazolate framework-8 (ZIF-8). Systematic characterization indicated 
      that the entrapment of lipase molecules was successfully achieved during the 
      crystal growth of ZIF-8 with an enzyme loading of  approximately 72.2 +/- 1.88 mg/g lipase@ZIF-8, 
      and the enzymes could facilitate the construction of framework building blocks. 
      Then the composite lipase@ZIF-8 was observed to possess favorable catalytic 
      activity and stability in the ester hydrolysis, using the hydrolysis of 
      p-nitrophenyl caprylate as a model. Finally, the composite was successfully 
      applied in the kinetic resolution of (R,S)-2-octanol, with favorable catalytic 
      activity and enantioselectivity during 10 cycle reactions. Thus, the biomimetic 
      mineralization process can be potentially used as an effective technique for 
      realizing the entrapment of biomacromolecules and constructing efficient 
      catalysts for industrial biocatalysis.
FAU - He, Hongming
AU  - He H
AD  - State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of 
      Chemistry, Jilin University , Changchun 130012, P. R. China.
FAU - Han, Haobo
AU  - Han H
AD  - Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, 
      School of Life Sciences, Jilin University , Changchun 130012, P. R. China.
FAU - Shi, Hui
AU  - Shi H
AD  - Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, 
      School of Life Sciences, Jilin University , Changchun 130012, P. R. China.
FAU - Tian, Yuyang
AU  - Tian Y
AD  - State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of 
      Chemistry, Jilin University , Changchun 130012, P. R. China.
FAU - Sun, Fuxing
AU  - Sun F
AD  - State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of 
      Chemistry, Jilin University , Changchun 130012, P. R. China.
FAU - Song, Yang
AU  - Song Y
AD  - State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of 
      Chemistry, Jilin University , Changchun 130012, P. R. China.
FAU - Li, Quanshun
AU  - Li Q
AD  - Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, 
      School of Life Sciences, Jilin University , Changchun 130012, P. R. China.
FAU - Zhu, Guangshan
AU  - Zhu G
AD  - State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of 
      Chemistry, Jilin University , Changchun 130012, P. R. China.
LA  - eng
PT  - Journal Article
DEP - 20160909
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
OTO - NOTNLM
OT  - biomimetic mineralization
OT  - ester hydrolysis
OT  - kinetic resolution
OT  - metal-organic frameworks
OT  - thermophilic lipase
EDAT- 2016/09/01 06:00
MHDA- 2016/09/01 06:01
CRDT- 2016/09/01 06:00
PHST- 2016/09/01 06:00 [entrez]
PHST- 2016/09/01 06:00 [pubmed]
PHST- 2016/09/01 06:01 [medline]
AID - 10.1021/acsami.6b05538 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2016 Sep 21;8(37):24517-24. doi: 
      10.1021/acsami.6b05538. Epub 2016 Sep 9.