PMID- 27586649
OWN - NLM
STAT- MEDLINE
DCOM- 20170626
LR  - 20230728
IS  - 1522-1547 (Electronic)
IS  - 0193-1857 (Print)
IS  - 0193-1857 (Linking)
VI  - 311
IP  - 4
DP  - 2016 Oct 1
TI  - Bacterial nutrient foraging in a mouse model of enteral nutrient deprivation: 
      insight into the gut origin of sepsis.
PG  - G734-G743
LID - 10.1152/ajpgi.00088.2016 [doi]
AB  - Total parenteral nutrition (TPN) leads to a shift in small intestinal microbiota 
      with a characteristic dominance of Proteobacteria This study examined how 
      metabolomic changes within the small bowel support an altered microbial community 
      in enterally deprived mice. C57BL/6 mice were given TPN or enteral chow. 
      Metabolomic analysis of jejunal contents was performed by liquid 
      chromatography/mass spectrometry (LC/MS). In some experiments, leucine in TPN was 
      partly substituted with [(13)C]leucine. Additionally, jejunal contents from 
      TPN-dependent and enterally fed mice were gavaged into germ-free mice to reveal 
      whether the TPN phenotype was transferrable. Small bowel contents of TPN mice 
      maintained an amino acid composition similar to that of the TPN solution. Mass 
      spectrometry analysis of small bowel contents of TPN-dependent mice showed 
      increased concentration of (13)C compared with fed mice receiving saline enriched 
      with [(13)C]leucine. [(13)C]leucine added to the serosal side of Ussing chambers 
      showed rapid permeation across TPN-dependent jejunum, suggesting increased 
      transmucosal passage. Single-cell analysis by fluorescence in situ hybridization 
      (FISH)-NanoSIMS demonstrated uptake of [(13)C]leucine by TPN-associated bacteria, 
      with preferential uptake by Enterobacteriaceae Gavage of small bowel effluent 
      from TPN mice into germ-free, fed mice resulted in a trend toward the 
      proinflammatory TPN phenotype with loss of epithelial barrier function. TPN 
      dependence leads to increased permeation of TPN-derived nutrients into the small 
      intestinal lumen, where they are predominately utilized by Enterobacteriaceae The 
      altered metabolomic composition of the intestinal lumen during TPN promotes 
      dysbiosis.
CI  - Copyright (c) 2016 the American Physiological Society.
FAU - Ralls, Matthew W
AU  - Ralls MW
AD  - Department of Surgery, Section of Pediatric Surgery, University of Michigan, Ann 
      Arbor, Michigan; mralls@med.umich.edu.
FAU - Demehri, Farokh R
AU  - Demehri FR
AD  - Department of Surgery, Section of Pediatric Surgery, University of Michigan, Ann 
      Arbor, Michigan.
FAU - Feng, Yongjia
AU  - Feng Y
AD  - Department of Surgery, Section of Pediatric Surgery, University of Michigan, Ann 
      Arbor, Michigan.
FAU - Raskind, Sasha
AU  - Raskind S
AD  - Michigan Regional Comprehensive Metabolomics Resource Core, University of 
      Michigan, Ann Arbor, Michigan.
FAU - Ruan, Chunhai
AU  - Ruan C
AD  - Michigan Regional Comprehensive Metabolomics Resource Core, University of 
      Michigan, Ann Arbor, Michigan.
FAU - Schintlmeister, Arno
AU  - Schintlmeister A
AD  - Division of Microbial Ecology, Department of Microbiology and Ecosystem Science, 
      Research Network Chemistry Meets Microbiology, University of Vienna, Vienna, 
      Austria.
AD  - Large-Instrument Facility for Advanced Isotope Research, University of Vienna, 
      Vienna, Austria; and.
FAU - Loy, Alexander
AU  - Loy A
AD  - Division of Microbial Ecology, Department of Microbiology and Ecosystem Science, 
      Research Network Chemistry Meets Microbiology, University of Vienna, Vienna, 
      Austria.
FAU - Hanson, Buck
AU  - Hanson B
AD  - Division of Microbial Ecology, Department of Microbiology and Ecosystem Science, 
      Research Network Chemistry Meets Microbiology, University of Vienna, Vienna, 
      Austria.
FAU - Berry, David
AU  - Berry D
AD  - Division of Microbial Ecology, Department of Microbiology and Ecosystem Science, 
      Research Network Chemistry Meets Microbiology, University of Vienna, Vienna, 
      Austria.
FAU - Burant, Charles F
AU  - Burant CF
AD  - Michigan Regional Comprehensive Metabolomics Resource Core, University of 
      Michigan, Ann Arbor, Michigan.
AD  - Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan.
FAU - Teitelbaum, Daniel H
AU  - Teitelbaum DH
AD  - Department of Surgery, Section of Pediatric Surgery, University of Michigan, Ann 
      Arbor, Michigan.
LA  - eng
GR  - P30 DK089503/DK/NIDDK NIH HHS/United States
GR  - R01 AI044076/AI/NIAID NIH HHS/United States
GR  - U24 DK097153/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20160901
PL  - United States
TA  - Am J Physiol Gastrointest Liver Physiol
JT  - American journal of physiology. Gastrointestinal and liver physiology
JID - 100901227
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Gastrointestinal Microbiome/*physiology
MH  - Intestinal Mucosa/*metabolism/microbiology
MH  - Jejunum/*metabolism/microbiology
MH  - Male
MH  - Metabolome
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - *Parenteral Nutrition, Total
MH  - Sepsis/*metabolism/microbiology
PMC - PMC5142194
OTO - NOTNLM
OT  - NanoSIMS
OT  - epithelial barrier function
OT  - metabolome
OT  - microbiota
OT  - nutrient foraging
OT  - total parenteral nutrition
EDAT- 2016/09/03 06:00
MHDA- 2017/06/27 06:00
PMCR- 2017/10/01
CRDT- 2016/09/03 06:00
PHST- 2016/02/29 00:00 [received]
PHST- 2016/08/22 00:00 [accepted]
PHST- 2016/09/03 06:00 [pubmed]
PHST- 2017/06/27 06:00 [medline]
PHST- 2016/09/03 06:00 [entrez]
PHST- 2017/10/01 00:00 [pmc-release]
AID - ajpgi.00088.2016 [pii]
AID - GI-00088-2016 [pii]
AID - 10.1152/ajpgi.00088.2016 [doi]
PST - ppublish
SO  - Am J Physiol Gastrointest Liver Physiol. 2016 Oct 1;311(4):G734-G743. doi: 
      10.1152/ajpgi.00088.2016. Epub 2016 Sep 1.