PMID- 27588757
OWN - NLM
STAT- MEDLINE
DCOM- 20170801
LR  - 20220408
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 11
IP  - 9
DP  - 2016
TI  - Platelet Supernatant Suppresses LPS-Induced Nitric Oxide Production from 
      Macrophages Accompanied by Inhibition of NF-kappaB Signaling and Increased Arginase-1 
      Expression.
PG  - e0162208
LID - 10.1371/journal.pone.0162208 [doi]
LID - e0162208
AB  - We previously reported that mouse bone marrow-derived macrophages (BMDMs) that 
      had been co-cultured with platelets exhibited lower susceptibility to bacterial 
      lipopolysaccharide (LPS) and produced lower levels of nitric oxide (NO) and 
      inflammatory cytokines including TNF-alpha and IL-6. The suppression of macrophage 
      responses was mediated, at least in part, by platelet supernatant. In the present 
      study, we assessed phenotypic changes of BMDMs induced by incubation with the 
      supernatant from thrombin-activated platelets (PLT-sup) and found that BMDMs 
      cultured with PLT-sup (PLT-BMDMs) expressed a lower level of inducible NO 
      synthase (iNOS) and a higher level of arginase-1, both of which are involved in 
      the L-arginine metabolism, upon stimulation with LPS or zymosan. We also examined 
      possible modulation of the NF-kappaB signaling pathway and observed suppression of 
      IkappaBalpha phosphorylation and a decrease of NF-kappaB p65 expression in LPS-stimulated 
      PLT-BMDMs. These results suggest that PLT-sup suppresses inflammatory responses 
      of BMDMs via negative regulation of NF-kappaB signaling leading to lowered expression 
      of iNOS and enhanced L-arginine catabolism by arginase-1.
FAU - Ando, Yusuke
AU  - Ando Y
AD  - Department of Microbiology, Hoshi University School of Pharmacy and 
      Pharmaceutical Sciences, Tokyo, Japan.
FAU - Oku, Teruaki
AU  - Oku T
AD  - Department of Microbiology, Hoshi University School of Pharmacy and 
      Pharmaceutical Sciences, Tokyo, Japan.
FAU - Tsuji, Tsutomu
AU  - Tsuji T
AUID- ORCID: 0000-0001-7920-0336
AD  - Department of Microbiology, Hoshi University School of Pharmacy and 
      Pharmaceutical Sciences, Tokyo, Japan.
LA  - eng
PT  - Journal Article
DEP - 20160902
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 3.5.3.1 (Arg1 protein, mouse)
RN  - EC 3.5.3.1 (Arginase)
SB  - IM
MH  - Animals
MH  - Arginase/*metabolism
MH  - Blood Platelets/*metabolism
MH  - Coculture Techniques
MH  - Lipopolysaccharides/*pharmacology
MH  - Macrophages/*drug effects/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - NF-kappa B/*metabolism
MH  - Nitric Oxide/*metabolism
MH  - Signal Transduction/*drug effects
PMC - PMC5010197
COIS- The authors have declared that no competing interests exist.
EDAT- 2016/09/03 06:00
MHDA- 2017/08/02 06:00
PMCR- 2016/09/02
CRDT- 2016/09/03 06:00
PHST- 2016/05/19 00:00 [received]
PHST- 2016/08/18 00:00 [accepted]
PHST- 2016/09/03 06:00 [entrez]
PHST- 2016/09/03 06:00 [pubmed]
PHST- 2017/08/02 06:00 [medline]
PHST- 2016/09/02 00:00 [pmc-release]
AID - PONE-D-16-20239 [pii]
AID - 10.1371/journal.pone.0162208 [doi]
PST - epublish
SO  - PLoS One. 2016 Sep 2;11(9):e0162208. doi: 10.1371/journal.pone.0162208. 
      eCollection 2016.