PMID- 27589211
OWN - NLM
STAT- MEDLINE
DCOM- 20170505
LR  - 20170813
IS  - 1879-2448 (Electronic)
IS  - 0043-1354 (Linking)
VI  - 104
DP  - 2016 Nov 1
TI  - Granulation of sulfur-oxidizing bacteria for autotrophic denitrification.
PG  - 507-519
LID - S0043-1354(16)30649-2 [pii]
LID - 10.1016/j.watres.2016.08.049 [doi]
AB  - Sulfur-oxidizing bacteria (SOB) was successfully employed for effective 
      autotrophic denitrification and sludge minimization in a full-scale application 
      of saline sewage treatment in Hong Kong. In this study, a Granular Sludge 
      Autotrophic Denitrification (GSAD) reactor was continuously operated over 600 
      days for SOB granulation, and to evaluate the long-term stability of SOB 
      granules, microbial communities and denitrification efficacy. Sludge granulation 
      initiated within the first 40 days of start-up with an average particle size of 
      186.4 mum and sludge volume index (SVI(5)) of 40 mL/g in 5 min. The sludge 
      granules continued to grow reaching a nearly uniform size of mean diameter 
      1380 +/- 20 mum with SVI(5) of 30 mL/g during 600 days of GSAD reactor operation at 
      hydraulic retention time of 5 h and nitrate loading rate of 0.33 kg-N/m(3)/d. The 
      GSAD reactor with SOB granular sludge achieved 93.7 +/- 2.1% nitrogen and complete 
      sulfide removal with low sludge yield of 0.15 g-volatile suspended solids 
      (VSS)/g-N, and much lower nitrous oxide (N(2)O) emission than the heterotrophic 
      denitrifying process. Microbial community analysis using fluorescence in situ 
      hybridization (FISH) technique revealed that granules were enriched with SOB 
      contributing to autotrophic denitrification. Furthermore, 16S rRNA analysis 
      showed diverse autotrophic denitrification related genera, namely Thiobacillus 
      (32.6%), Sulfurimonas (31.3%), and Arcobacter (0.01%), accounting for 63.9% of 
      total operational taxonomic units at the generic level. No heterotrophic 
      denitrification related genera were detected. The results from this study could 
      provide useful design and operating conditions with respect to SOB sludge 
      granulation and its subsequent application in a full-scale autotrophic 
      denitrification in the Sulfate reduction-Autotrophic 
      denitrification-Nitrification Integrated (SANI) process.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Yang, Weiming
AU  - Yang W
AD  - School of Environmental Science and Engineering, Sun Yat-sen University, 
      Guangzhou, China; Guangdong Provincial Key Laboratory of Environmental Pollution 
      Control and Remediation Technology (Sun Yat-sen University), Guangzhou, China.
FAU - Lu, Hui
AU  - Lu H
AD  - School of Environmental Science and Engineering, Sun Yat-sen University, 
      Guangzhou, China; Guangdong Provincial Key Laboratory of Environmental Pollution 
      Control and Remediation Technology (Sun Yat-sen University), Guangzhou, China. 
      Electronic address: lvhui3@mail.sysu.edu.cn.
FAU - Khanal, Samir K
AU  - Khanal SK
AD  - Department of Molecular Biosciences and Bioengineering, University of Hawaii at 
      Manoa, USA.
FAU - Zhao, Qing
AU  - Zhao Q
AD  - School of Civil Engineering, Guangzhou University, Guangzhou, China.
FAU - Meng, Liao
AU  - Meng L
AD  - Shenzhen Fengrun Environmental Technology Co., Ltd, Shenzhen, China.
FAU - Chen, Guang-Hao
AU  - Chen GH
AD  - School of Environmental Science and Engineering, Sun Yat-sen University, 
      Guangzhou, China; Guangdong Provincial Key Laboratory of Environmental Pollution 
      Control and Remediation Technology (Sun Yat-sen University), Guangzhou, China; 
      Department of Civil & Environmental Engineering, Hong Kong University of Science 
      and Technology, Hong Kong, China.
LA  - eng
PT  - Journal Article
DEP - 20160824
PL  - England
TA  - Water Res
JT  - Water research
JID - 0105072
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
RN  - 0 (Sulfates)
RN  - 70FD1KFU70 (Sulfur)
SB  - IM
MH  - Autotrophic Processes
MH  - Bacteria/genetics
MH  - Bioreactors
MH  - *Denitrification
MH  - In Situ Hybridization, Fluorescence
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sewage/microbiology
MH  - Sulfates
MH  - *Sulfur
OTO - NOTNLM
OT  - Autotrophic denitrification
OT  - Granulation
OT  - Microbial community analysis
OT  - Sulfur-oxidizing bacteria
EDAT- 2016/09/03 06:00
MHDA- 2017/05/06 06:00
CRDT- 2016/09/03 06:00
PHST- 2016/04/27 00:00 [received]
PHST- 2016/08/19 00:00 [revised]
PHST- 2016/08/23 00:00 [accepted]
PHST- 2016/09/03 06:00 [pubmed]
PHST- 2017/05/06 06:00 [medline]
PHST- 2016/09/03 06:00 [entrez]
AID - S0043-1354(16)30649-2 [pii]
AID - 10.1016/j.watres.2016.08.049 [doi]
PST - ppublish
SO  - Water Res. 2016 Nov 1;104:507-519. doi: 10.1016/j.watres.2016.08.049. Epub 2016 
      Aug 24.