PMID- 27590857
OWN - NLM
STAT- MEDLINE
DCOM- 20171117
LR  - 20211204
IS  - 1878-5875 (Electronic)
IS  - 1357-2725 (Linking)
VI  - 79
DP  - 2016 Oct
TI  - Akirin2 could promote the proliferation but not the differentiation of duck 
      myoblasts via the activation of the mTOR/p70S6K signaling pathway.
PG  - 298-307
LID - S1357-2725(16)30249-7 [pii]
LID - 10.1016/j.biocel.2016.08.032 [doi]
AB  - The Akirin gene family normally contains two members that are essential to 
      myoblast differentiation. Noticeably, the avian Akirin gene family comprises only 
      one gene (Akirin2), However, it remains unknown whether avian Akirin gene family 
      still has the function of Akirin1; moreover, it is still unclear whether and how 
      Akirin2 plays a role in myoblast proliferation and differentiation. 
      Interestingly, the unexpected functions of duck Akirin2 were revealed in the 
      present study. The Real-time PCR results showed that between 12 and 48h during 
      the process of duck myoblasts differentiation, the overexpression of Akirin2 did 
      not significantly increase the expression of myogenic regulatory factors. Flow 
      cytometry analysis revealed that the cell cycle transition was accelerated by 
      Akirin2 overexpression. Moreover, the overexpression of Akirin2 did not influence 
      the myotube formation. Strikingly, when duck myoblasts were cultured in the 
      growth medium, the overexpression of Akirin2 significantly enhanced cell 
      viability. Although the expression of cyclin-dependent proteins did not 
      significantly increase after transfection, the expression of the mammalian 
      targets of rapamycin (mTOR) and p70 S6 kinase (p70S6K) increased. Furthermore, 
      the protein expression of phospho-p70S6K (Ser 417) also increased. However, when 
      rapamycin and pEGFP-N1-Akirin2 plasmids were added together to the growth medium, 
      the positive impact of Akirin2 on cell viability and the mRNA expression of mTOR 
      and p70S6K were significantly blocked. Furthermore, the expression of 
      phospho-mTOR (Ser 2448) and phospho-p70S6K (Ser 417) were also blocked. Taken 
      together, these results could suggest that duck Akirin2 could promote myoblast 
      proliferation via the activation of the mTOR/p70S6K signaling pathway.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Sun, Wenqiang
AU  - Sun W
AD  - Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of 
      Sichuan Province, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR 
      China.
FAU - Huang, Huilan
AU  - Huang H
AD  - Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of 
      Sichuan Province, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR 
      China.
FAU - Ma, Shengchao
AU  - Ma S
AD  - Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of 
      Sichuan Province, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR 
      China.
FAU - Gan, Xiang
AU  - Gan X
AD  - Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of 
      Sichuan Province, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR 
      China.
FAU - Zhu, Mou
AU  - Zhu M
AD  - Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of 
      Sichuan Province, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR 
      China.
FAU - Liu, Hehe
AU  - Liu H
AD  - Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of 
      Sichuan Province, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR 
      China.
FAU - Li, Liang
AU  - Li L
AD  - Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of 
      Sichuan Province, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR 
      China.
FAU - Wang, Jiwen
AU  - Wang J
AD  - Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of 
      Sichuan Province, Sichuan Agricultural University, Ya'an, Sichuan 625014, PR 
      China. Electronic address: wjw2886166@163.com.
LA  - eng
PT  - Journal Article
DEP - 20160831
PL  - Netherlands
TA  - Int J Biochem Cell Biol
JT  - The international journal of biochemistry & cell biology
JID - 9508482
RN  - 0 (Myogenic Regulatory Factors)
RN  - 0 (Repressor Proteins)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Animals
MH  - Cell Cycle Checkpoints/drug effects
MH  - *Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Ducks
MH  - Gene Expression Regulation/drug effects
MH  - Myoblasts/*cytology/drug effects/metabolism
MH  - Myogenic Regulatory Factors/genetics
MH  - Repressor Proteins/*metabolism
MH  - Ribosomal Protein S6 Kinases, 70-kDa/*metabolism
MH  - *Signal Transduction/drug effects
MH  - Sirolimus/pharmacology
MH  - TOR Serine-Threonine Kinases/*metabolism
MH  - Transcription, Genetic/drug effects
OTO - NOTNLM
OT  - Differentiation
OT  - Duck Akirin2
OT  - Duck myoblast
OT  - Proliferation
OT  - mTOR/p70S6K
EDAT- 2016/09/04 06:00
MHDA- 2017/11/29 06:00
CRDT- 2016/09/04 06:00
PHST- 2016/05/05 00:00 [received]
PHST- 2016/08/08 00:00 [revised]
PHST- 2016/08/29 00:00 [accepted]
PHST- 2016/09/04 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2016/09/04 06:00 [entrez]
AID - S1357-2725(16)30249-7 [pii]
AID - 10.1016/j.biocel.2016.08.032 [doi]
PST - ppublish
SO  - Int J Biochem Cell Biol. 2016 Oct;79:298-307. doi: 10.1016/j.biocel.2016.08.032. 
      Epub 2016 Aug 31.