PMID- 27591111
OWN - NLM
STAT- MEDLINE
DCOM- 20170330
LR  - 20171008
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 100
DP  - 2016 Nov
TI  - Plasminogen coating increases initial adhesion of oral bacteria in vitro.
PG  - 10-16
LID - S0882-4010(16)30016-X [pii]
LID - 10.1016/j.micpath.2016.08.002 [doi]
AB  - Plasminogen is a major plasma protein and the zymogen of the broad spectrum 
      protease plasmin. Plasmin activity leads to tissue degradation, direct and 
      through activation of metalloproteinases. Infected tooth root canals, as a 
      consequence of the inflammatory response and eventual necrosis, contain tissue 
      fluid and blood components. These will coat the root canal walls and act as 
      conditioning films that allow bacterial biofilms to grow and be a potential 
      source of hematogenously spreading bacteria. We investigated the effect of 
      in vitro surface conditioning with human plasminogen on the initial adhesion of 
      bacteria. Four bacterial species, L. salivarius, E. faecalis, A. naeslundii, and 
      S. gordonii, isolated from dental root canals, and three other oral streptococci, 
      S. oralis, S. anginosus, and S. sanguinis, were grown in albumin- or 
      plasminogen-coated flow chambers and studied by confocal laser scanning 
      microscopy using the cell viability staining LIVE/DEAD and 16S rRNA fluorescence 
      in situ hybridization (FISH). A. naeslundii, L. salivarius and in particular 
      S. gordonii showed a higher initial adhesion to the plasminogen-coated surfaces. 
      E. faecalis did not show any preference for plasminogen. Four-species biofilms 
      cultured for 96 h showed that streptococci increased their proportion with time. 
      Further experiments aimed at studying different streptococcal strains. All these 
      adhered more to plasminogen-coated surfaces than to albumin-coated control 
      surfaces. The specificity of the binding to plasminogen was verified by blocking 
      lysine-binding sites with epsilon-aminocaproic acid. Plasminogen is thus an 
      important plasma component for the initial adhesion of oral bacteria, in 
      particular streptococci. This binding may contribute to their spread locally as 
      well as to distant organs or tissues.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Kinnby, Bertil
AU  - Kinnby B
AD  - Department of Oral Biology, Faculty of Odontology, Malmo University College, 
      S-20506, Malmo, Sweden. Electronic address: Bertil.Kinnby@mah.se.
FAU - Chavez de Paz, Luis E
AU  - Chavez de Paz LE
AD  - Department of Oral Biology, Faculty of Odontology, Malmo University College, 
      S-20506, Malmo, Sweden.
LA  - eng
PT  - Journal Article
DEP - 20160830
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Fibrinolytic Agents)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 9001-91-6 (Plasminogen)
SB  - IM
MH  - *Bacterial Adhesion
MH  - Cluster Analysis
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Fibrinolytic Agents
MH  - Gram-Positive Bacteria/*physiology
MH  - *Host-Pathogen Interactions
MH  - Humans
MH  - Phylogeny
MH  - Plasminogen/*metabolism
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
OTO - NOTNLM
OT  - Gingiva
OT  - Gram-negative bacteria
OT  - Host defense
OT  - Host-pathogen interaction
OT  - Mucosal immunology
OT  - Plasminogen
EDAT- 2016/10/28 06:00
MHDA- 2017/03/31 06:00
CRDT- 2016/09/04 06:00
PHST- 2016/01/08 00:00 [received]
PHST- 2016/06/22 00:00 [revised]
PHST- 2016/08/02 00:00 [accepted]
PHST- 2016/10/28 06:00 [pubmed]
PHST- 2017/03/31 06:00 [medline]
PHST- 2016/09/04 06:00 [entrez]
AID - S0882-4010(16)30016-X [pii]
AID - 10.1016/j.micpath.2016.08.002 [doi]
PST - ppublish
SO  - Microb Pathog. 2016 Nov;100:10-16. doi: 10.1016/j.micpath.2016.08.002. Epub 2016 
      Aug 30.