PMID- 2759243
OWN - NLM
STAT- MEDLINE
DCOM- 19890912
LR  - 20190621
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 253
IP  - 1-2
DP  - 1989 Aug 14
TI  - Linker histone-DNA complexes: enhanced stability in the presence of aluminum 
      lactate and implications for Alzheimer's disease.
PG  - 59-62
AB  - The binding of human brain linker histone proteins to a radiolabelled human Alu 
      repetitive element was examined by mobility shift assay. Analysis of the 
      complexes formed from protein extracts of whole neocortical nuclei, under 
      physiological conditions in vitro revealed that linker histone H1(0) has the 
      highest affinity for the Alu DNA sequence. The linker histone-DNA complexes 
      assembled in the presence of aluminum lactate were more resistant to sodium 
      chloride-induced dissociation than those formed in the presence of sodium 
      lactate. The enhanced stability of deoxyribonucleoprotein (DNP) complexes in the 
      presence of the aluminum cation may be of significance in neurodegenerative 
      conditions such as Alzheimer's disease where aluminum preferentially associates 
      with DNA containing structures of the nucleus.
FAU - Lukiw, W J
AU  - Lukiw WJ
AD  - Collaborative Program in Neuroscience, University of Toronto, Canada.
FAU - Kruck, T P
AU  - Kruck TP
FAU - McLachlan, D R
AU  - McLachlan DR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Histones)
RN  - 0 (Lactates)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 451W47IQ8X (Sodium Chloride)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Alzheimer Disease/*physiopathology
MH  - DNA/*metabolism
MH  - DNA-Binding Proteins/*metabolism
MH  - Histones/*metabolism
MH  - Humans
MH  - In Vitro Techniques
MH  - Lactates/*pharmacology
MH  - Lactic Acid
MH  - Protein Binding/drug effects
MH  - Repetitive Sequences, Nucleic Acid
MH  - Sodium Chloride/pharmacology
EDAT- 1989/08/14 00:00
MHDA- 1989/08/14 00:01
CRDT- 1989/08/14 00:00
PHST- 1989/08/14 00:00 [pubmed]
PHST- 1989/08/14 00:01 [medline]
PHST- 1989/08/14 00:00 [entrez]
AID - 0014-5793(89)80929-9 [pii]
AID - 10.1016/0014-5793(89)80929-9 [doi]
PST - ppublish
SO  - FEBS Lett. 1989 Aug 14;253(1-2):59-62. doi: 10.1016/0014-5793(89)80929-9.