PMID- 27592536 OWN - NLM STAT- MEDLINE DCOM- 20170926 LR - 20180321 IS - 1096-0805 (Electronic) IS - 0022-2011 (Linking) VI - 140 DP - 2016 Oct TI - Evidence of vertical transmission of ostreid herpesvirus 1 in the Portuguese oyster Crassostrea angulata. PG - 39-41 LID - S0022-2011(16)30124-0 [pii] LID - 10.1016/j.jip.2016.08.012 [doi] AB - In order to determine if ostreid herpesvirus 1 (OsHV-1) can be vertically transmitted, 9 full-sib families of the Portuguese oyster Crassostrea angulata were produced using a factorial mating design with 3 males and 3 females. The parents were survivors from an OsHV-1 mortality outbreak. OsHV-1 DNA was not detected by conventional PCR in the mantle of parents, gametes or 3day-old larvae. However, viral DNA was detected by real-time PCR in all gametes and larvae samples. These results show that C. angulata that have survived an OsHV-1 mortality outbreak can carry the virus and vertically transmit it to their offspring. CI - Copyright (c) 2016 Elsevier Inc. All rights reserved. FAU - Lopez Sanmartin, Monserrat AU - Lopez Sanmartin M AD - Instituto de Investigacion y Formacion Agraria y Pesquera (IFAPA), Centro Agua del Pino, Junta de Andalucia, Ctra. El Rompido-Punta Umbria, km 4, 21459 Cartaya, Spain. Electronic address: monserratls@yahoo.es. FAU - Power, Deborah M AU - Power DM AD - Centro de Ciencias do Mar (CCMAR), Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal. FAU - de la Herran, Roberto AU - de la Herran R AD - Departamento de Genetica de la Universidad de Granada, Spain. FAU - Navas, Jose I AU - Navas JI AD - Instituto de Investigacion y Formacion Agraria y Pesquera (IFAPA), Centro Agua del Pino, Junta de Andalucia, Ctra. El Rompido-Punta Umbria, km 4, 21459 Cartaya, Spain. FAU - Batista, Frederico M AU - Batista FM AD - Centro de Ciencias do Mar (CCMAR), Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal; Instituto Portugues do Mar e da Atmosfera, Divisao de Aquicultura e Valorizacao, Estacao Experimental de Moluscicultura de Tavira, Av. 5 de Outubro, 8700-305 Olhao, Portugal. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20160901 PL - United States TA - J Invertebr Pathol JT - Journal of invertebrate pathology JID - 0014067 SB - IM MH - Animals MH - Crassostrea/*virology MH - Female MH - Herpesviridae MH - Herpesviridae Infections/*veterinary MH - Infectious Disease Transmission, Vertical MH - Larva/virology MH - Male MH - Real-Time Polymerase Chain Reaction OTO - NOTNLM OT - Crassostrea angulata OT - Offspring OT - OsHV-1 OT - Swim up OT - Vertical transmission EDAT- 2016/09/07 06:00 MHDA- 2017/09/28 06:00 CRDT- 2016/09/06 06:00 PHST- 2016/06/15 00:00 [received] PHST- 2016/08/25 00:00 [revised] PHST- 2016/08/31 00:00 [accepted] PHST- 2016/09/07 06:00 [pubmed] PHST- 2017/09/28 06:00 [medline] PHST- 2016/09/06 06:00 [entrez] AID - S0022-2011(16)30124-0 [pii] AID - 10.1016/j.jip.2016.08.012 [doi] PST - ppublish SO - J Invertebr Pathol. 2016 Oct;140:39-41. doi: 10.1016/j.jip.2016.08.012. Epub 2016 Sep 1.