PMID- 27592569
OWN - NLM
STAT- MEDLINE
DCOM- 20170329
LR  - 20181202
IS  - 1672-7347 (Print)
IS  - 1672-7347 (Linking)
VI  - 41
IP  - 7
DP  - 2016 Jul
TI  - [Genetic abnormality in 101 cases of plasma cell dyscrasias by FISH technology 
      and cytogenetic examination].
PG  - 668-75
LID - 10.11817/j.issn.1672-7347.2016.07.002 [doi]
AB  - OBJECTIVE: To investigate several abnormal genes by the fluorescence in situ 
      hybridization (FISH) in multiple myeloma (MM), monoclonal gammopathy of 
      undetermined significance (MGUS) and reactive plasmacytosis (RP), and to increase 
      the diagnosis and differential diagnosis levels for these common plasma diseases. 
      
 METHODS: The clinical manifestations, image and laboratory tests and the FISH 
      detection were retrospectively analyzed in 61 cases of newly diagnosed MM, 20 
      cases of MGUS and 20 cases of RP from August, 2012 to February, 2015 in the 
      Xiangya Hospital of Central South University. 
 RESULTS: Fifty cases among 61 MM 
      patients showed genetic abnormality by FISH technology. The total positive rate 
      was 81.9%. Among them, 19 cases (31.1%) had 1q21 amplification, 18 cases (29.5%) 
      lacked D13S319, 10 cases (16.4%) missed RB1, 10 cases (16.4%) had IGH 
      translocation and 7 cases (11.4%) lacked p53 gene. The positive rate for two or 
      more genes abnormal was 19.8% in 12 cases. However, in 20 cases of MGUS patients, 
      the positive detection rate was 25%, including 4 cases (20%) of 1q21 augmentation 
      and 2 cases (10%) of IGH translocation. There were not two or more abnormal genes 
      in one case. While in RP cases, only 1 case of patients had D13S319 abnormal 
      gene, and the positive rate was only 5%. There was significant difference 
      (P<0.05) among the 3 groups. 
 CONCLUSION: The positive detection rate is 81.9% 
      in MM patients by FISH, which is significantly higher than that in patients with 
      MGUS or RP. FISH technology can detect a variety of abnormal genes in MM. It is 
      useful for the differential diagnosis and prognosis for MM, MGUS and RP.
FAU - Cao, Pengfei
AU  - Cao P
AD  - Department of Hematology, Xianya Hospital, Central South University, Changsha 
      410008; Cancer Research Institute, Central South University, Changsha 410078, 
      China.
FAU - Li, Guiyuan
AU  - Li G
AD  - Cancer Research Institute, Central South University, Changsha 410078, China.
FAU - Tan, Qian
AU  - Tan Q
AD  - Department of Hematology, Xianya Hospital, Central South University, Changsha 
      410008, China.
FAU - Zhang, Ying
AU  - Zhang Y
AD  - Department of Hematology, Xianya Hospital, Central South University, Changsha 
      410008, China.
FAU - Zhang, Guoping
AU  - Zhang G
AD  - Department of Hematology, Xianya Hospital, Central South University, Changsha 
      410008, China.
FAU - Li, Xiaolin
AU  - Li X
AD  - Department of Hematology, Xianya Hospital, Central South University, Changsha 
      410008, China.
FAU - He, Yuxiang
AU  - He Y
AD  - Department of Oncology, Xianya Hospital, Central South University, Changsha 
      410008, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhong Nan Da Xue Xue Bao Yi Xue Ban
JT  - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. 
      Medical sciences
JID - 101230586
SB  - IM
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Monoclonal Gammopathy of Undetermined Significance
MH  - Multiple Myeloma
MH  - *Paraproteinemias
MH  - Retrospective Studies
MH  - Translocation, Genetic
EDAT- 2016/09/07 06:00
MHDA- 2017/03/31 06:00
CRDT- 2016/09/06 06:00
PHST- 2016/09/06 06:00 [entrez]
PHST- 2016/09/07 06:00 [pubmed]
PHST- 2017/03/31 06:00 [medline]
AID - 10.11817/j.issn.1672-7347.2016.07.002 [doi]
PST - ppublish
SO  - Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2016 Jul;41(7):668-75. doi: 
      10.11817/j.issn.1672-7347.2016.07.002.