PMID- 27592863
OWN - NLM
STAT- MEDLINE
DCOM- 20180129
LR  - 20180129
IS  - 1613-6829 (Electronic)
IS  - 1613-6810 (Linking)
VI  - 12
IP  - 47
DP  - 2016 Dec
TI  - Organic Nanoparticles with Aggregation-Induced Emission for Bone Marrow Stromal 
      Cell Tracking in a Rat PTI Model.
PG  - 6576-6585
LID - 10.1002/smll.201601630 [doi]
AB  - Stem-cell based therapy is an emerging therapeutic approach for ischemic stroke 
      treatment. Bone marrow stromal cells (BMSCs) are in common use as a cell source 
      for stem cell therapy and show promising therapeutic outcomes for stroke 
      treatment. One challenge is to develop a reliable tracking strategy to monitor 
      the fate of BMSCs and assess their therapeutic effects in order to improve the 
      success rate of such treatment. Herein, TPEEP, a fluorogen with 
      aggregation-induced emission characteristics and near-infrared emission are 
      designed and synthesized and further fabricated into organic nanoparticles (NPs). 
      The obtained NPs show high fluorescence quantum yield, low cytotoxicity with good 
      physical and photostability, which display excellent tracking performance of 
      BMSCs in vitro and in vivo. Using a rat photothrombotic ischemia model as an 
      example, the NP-labeled BMSCs are able to migrate to the stroke lesion site to 
      yield bright red fluorescence. Immunofluorescence staining shows that the NP 
      labeling does not affect the normal function of BMSCs, proving their good 
      biocompatibility in vivo. These merits make TPEEP NP a potential cell tracker to 
      evaluate the fate of BMSCs in cell therapy.
CI  - (c) 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Cai, Xiaolei
AU  - Cai X
AD  - Department of Chemical and Biomolecular Engineering, National University of 
      Singapore, 4 Engineering Drive 4, Singapore, 117585.
AD  - NUS Graduate School for Integrative Sciences and Engineering, National University 
      of Singapore, 28 Medical Drive, #05-01, Singapore, 117456.
FAU - Zhang, Chong-Jing
AU  - Zhang CJ
AD  - Department of Chemical and Biomolecular Engineering, National University of 
      Singapore, 4 Engineering Drive 4, Singapore, 117585.
FAU - Ting Wei Lim, Frances
AU  - Ting Wei Lim F
AD  - Singapore Institute for Neurotechnology (SINAPSE), National University of 
      Singapore, 28 Medical Drive, Singapore, 117456.
FAU - Chan, Su Jing
AU  - Chan SJ
AD  - Neuroprotection Research Laboratory, Departments of Radiology and Neurology, 
      Massachusetts General Hospital and Harvard Medical School, Charlestown, MA, 2129, 
      USA.
FAU - Bandla, Aishwarya
AU  - Bandla A
AD  - Singapore Institute for Neurotechnology (SINAPSE), National University of 
      Singapore, 28 Medical Drive, Singapore, 117456.
FAU - Chuan, Chan Kim
AU  - Chuan CK
AD  - Singapore Institute for Neurotechnology (SINAPSE), National University of 
      Singapore, 28 Medical Drive, Singapore, 117456.
FAU - Hu, Fang
AU  - Hu F
AD  - Department of Chemical and Biomolecular Engineering, National University of 
      Singapore, 4 Engineering Drive 4, Singapore, 117585.
FAU - Xu, Shidang
AU  - Xu S
AD  - Department of Chemical and Biomolecular Engineering, National University of 
      Singapore, 4 Engineering Drive 4, Singapore, 117585.
FAU - Thakor, Nitish V
AU  - Thakor NV
AD  - Singapore Institute for Neurotechnology (SINAPSE), National University of 
      Singapore, 28 Medical Drive, Singapore, 117456.
FAU - Liao, Lun-De
AU  - Liao LD
AD  - Singapore Institute for Neurotechnology (SINAPSE), National University of 
      Singapore, 28 Medical Drive, Singapore, 117456.
AD  - Institute of Biomedical Engineering and Nanomedicine, National Health Research 
      Institutes, 35 Keyan Road, Zhunan Town, Miaoli County, 35053, Taiwan.
FAU - Liu, Bin
AU  - Liu B
AD  - Department of Chemical and Biomolecular Engineering, National University of 
      Singapore, 4 Engineering Drive 4, Singapore, 117585.
LA  - eng
PT  - Journal Article
DEP - 20160904
PL  - Germany
TA  - Small
JT  - Small (Weinheim an der Bergstrasse, Germany)
JID - 101235338
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/metabolism
MH  - Brain Ischemia/*diagnostic imaging
MH  - Disease Models, Animal
MH  - Fluorescent Dyes/chemical synthesis/*chemistry
MH  - Nanoparticles/*chemistry
MH  - Rats
OTO - NOTNLM
OT  - aggregation-induced emission (AIE)
OT  - bone marrow stromal cells
OT  - cell tracking
OT  - nanoparticles
OT  - photothrombotic ischemia
EDAT- 2016/09/07 06:00
MHDA- 2018/01/30 06:00
CRDT- 2016/09/06 06:00
PHST- 2016/05/13 00:00 [received]
PHST- 2016/06/24 00:00 [revised]
PHST- 2016/09/07 06:00 [pubmed]
PHST- 2018/01/30 06:00 [medline]
PHST- 2016/09/06 06:00 [entrez]
AID - 10.1002/smll.201601630 [doi]
PST - ppublish
SO  - Small. 2016 Dec;12(47):6576-6585. doi: 10.1002/smll.201601630. Epub 2016 Sep 4.