PMID- 27593543
OWN - NLM
STAT- MEDLINE
DCOM- 20171116
LR  - 20201209
IS  - 2212-3334 (Electronic)
IS  - 1872-2148 (Linking)
VI  - 10
IP  - 2
DP  - 2017
TI  - Real Time Polymerase Chain Reaction (rt-PCR): A New Patent to Diagnostic Purposes 
      for Paracoccidioidomycosis.
PG  - 143-149
LID - 10.2174/1872214810666160905150958 [doi]
AB  - BACKGROUND: Paracoccidioidomycosis (PCM) is a systemic mycosis caused by 
      dimorphic fungi Paracoccidioides brasiliensis and Paracoccidioides lutzii. It is 
      prevalent in Latin American, mainly in Brazil. Therefore, PCM has fundamental 
      impact on the Brazilian global economy, especially in public health system, since 
      it is affecting economical active population in different country regions. 
      OBJECTIVE: The present study aimed to standardize the Real Time-Polymerase Chain 
      Reaction (rt-PCR) for an efficient and safe PCM diagnosis amplifying the 
      recombinant protein PB27 gene, only expressed by specimens of Paracoccidioides 
      genus. METHODS: To standardize a methodology of rt-PCR using species-specific 
      primers and probe designed for annealing in this specific region of the fungi s 
      genome, amplifying the recombinant protein PB27 gene, only expressed by specimens 
      of Paracoccidioides genus. Followed by design in silico, experiments were 
      performed in vitro to determine rt-PCR specificity, efficiency and genome 
      detection limit. RESULTS: The primers and probe sequences were deposited in 
      Brazilian Coordination of Technological Innovation and Transfer (CTIT), under 
      patent reference number BR1020160078830. The present study demonstrated the 
      rt-PCR applicability for support on diagnosis of paracoccidioidomycosis, 
      presenting low cost, which makes it affordable for public health services in 
      developing countries as Brazil. It is noteworthy that it is necessary to validate 
      this methodology using clinical samples before to use as a safe method of 
      diagnosis. A review of all patents related to this topic was performed and it was 
      shown that, to date, there are no records of patent on kits for 
      paracoccidioidomycosis s diagnostic. Indeed, there is still a lot to go to reach 
      this goal. CONCLUSION: The reaction developed was standardized and patented, 
      opening perspectives to molecular diagnosis development for 
      paracoccidioidomycosis, since rt-PCR can be applied to a broad spectrum of 
      infectious diseases. It would need to be tested in biological samples in order to 
      validate this method and then generate a diagnostic kit for 
      Paracoccidioidomycosis.
CI  - Copyright(c) Bentham Science Publishers; For any queries, please email at 
      epub@benthamscience.org.
FAU - Rocha-Silva, Fabiana
AU  - Rocha-Silva F
AD  - Nucleo de Pos-Graduacao e Pesquisa da Santa Casa de Belo Horizonte, Minas Gerais. 
      Brazil.
FAU - Gomes, Luciana I
AU  - Gomes LI
AD  - Centro de Pesquisas Rene Rachou-FIOCRUZ, Belo Horizonte. Brazil.
FAU - Gracielle-Melo, Cidiane
AU  - Gracielle-Melo C
AD  - Nucleo de Pos-Graduacao e Pesquisa da Santa Casa de Belo Horizonte, Minas Gerais. 
      Brazil.
FAU - Goes, Alfredo M
AU  - Goes AM
AD  - Departamento de Bioquimica e Imunologia, Universidade Federal de Minas Gerais, 
      Minas Gerais. Brazil.
FAU - Caligiorne, Rachel B
AU  - Caligiorne RB
AD  - Rua Domingos Vieira, 590, CEP: 30150240 - Belo Horizonte, Minas Gerais. Brazil.
LA  - eng
PT  - Journal Article
PL  - United Arab Emirates
TA  - Recent Pat Endocr Metab Immune Drug Discov
JT  - Recent patents on endocrine, metabolic & immune drug discovery
JID - 101299743
RN  - 0 (Fungal Proteins)
RN  - 0 (Pb27 protein, Paracoccidioides brasiliensis)
SB  - IM
MH  - Brazil
MH  - Computer Simulation
MH  - Fungal Proteins/*genetics
MH  - Genome, Fungal
MH  - Humans
MH  - Paracoccidioides/genetics/*isolation & purification
MH  - Paracoccidioidomycosis/*diagnosis/microbiology
MH  - Patents as Topic
MH  - Real-Time Polymerase Chain Reaction/economics/*methods
MH  - Sensitivity and Specificity
MH  - Species Specificity
OTO - NOTNLM
OT  - Diagnosis
OT  - Paracocciodioides brasiliensis
OT  - Paracocciodioides lutzii
OT  - Pb 27 protein
OT  - paracoccidioidomycosis
OT  - real time-PCR.
EDAT- 2016/09/07 06:00
MHDA- 2017/11/29 06:00
CRDT- 2016/09/06 06:00
PHST- 2016/05/18 00:00 [received]
PHST- 2016/08/16 00:00 [revised]
PHST- 2016/09/01 00:00 [accepted]
PHST- 2016/09/07 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2016/09/06 06:00 [entrez]
AID - EMI-EPUB-78205 [pii]
AID - 10.2174/1872214810666160905150958 [doi]
PST - ppublish
SO  - Recent Pat Endocr Metab Immune Drug Discov. 2017;10(2):143-149. doi: 
      10.2174/1872214810666160905150958.