PMID- 27593871 OWN - NLM STAT- MEDLINE DCOM- 20170227 LR - 20181202 IS - 1976-2437 (Electronic) IS - 0513-5796 (Print) IS - 0513-5796 (Linking) VI - 57 IP - 6 DP - 2016 Nov TI - Different Responses in Induction of Allergen Specific Immunoglobulin G4 and IgE-Blocking Factors for Three Mite Subcutaneous Immunotherapy Products. PG - 1427-34 LID - 10.3349/ymj.2016.57.6.1427 [doi] AB - PURPOSE: Specific immunoglobulin G4 (sIgG4) and immunoglobulin E (IgE)-blocking factors produced by subcutaneous immunotherapy (SCIT) play a critical role in the induction of allergen tolerance. However, comparative studies of available SCIT reagents on the induction of sIgG4 are limited. We compared increases in sIgG4 for three different house dust mite (HDM) SCIT reagents. MATERIALS AND METHODS: Seventy-two HDM sensitized allergic patients were enrolled and classified into four groups: 1) control (n=27), 2) SCIT with Hollister-Stier(R) (n=19), 3) Tyrosine S(R) (n=16), and 4) Novo-Helisen(R) (n=10). Levels of specific IgE (sIgE), sIgG4, and IgE blocking factor to Dermatophagoides farinae (D. farinae) were measured using ImmunoCAP (sIgE, sIgG4) and enzyme-linked immunosorbent assay (ELISA) (IgE-blocking factors). Levels were measured before and 13.9+/-6.6 months after the SCIT. The allergen specificity and the induction levels of sIgE and sIgG4 were confirmed by immunoblot analysis. RESULTS: After SCIT, sIgG4 levels to D. farinae increased significantly; however, the increases differed significantly among the SCIT groups (p<0.001). Specific IgG4 levels to D. farinae were highest in Hollister-Stier(R) (3.7+/-4.1 mg/L), followed by Novo-Helisen(R) (2.2+/-2.3 mg/L) and Tyrosine S(R) (0.7+/-0.5 mg/L). In addition, patients who were administered using Hollister-Stier(R) showed the most significant decrease in IgE/IgG4 ratio (p<0.001) and increase in blocking factor (p=0.009). Finally, according to IgE immunoblot results, the Hollister-Stier(R) group showed the most significant attenuation of IgE binding patterns among others. CONCLUSION: Currently available SCIT reagents induce different levels of specific IgG4, IgE/IgG4 ratio, and IgE-blocking factor. FAU - Park, Kyung Hee AU - Park KH AD - Division of Allergy and Immunology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea. AD - Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea. FAU - Lee, Sang Chul AU - Lee SC AD - Division of Allergy and Immunology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea. FAU - Son, Young Woong AU - Son YW AD - Division of Allergy and Immunology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea. FAU - Jeong, Kyoung Yong AU - Jeong KY AD - Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea. FAU - Shin, Yoo Seob AU - Shin YS AD - Department of Allergy and Clinical Immunology, Ajou Medical School of Medicine, Suwon, Korea. FAU - Shin, Jung U AU - Shin JU AD - Department of Dermatology & Cutaneous Biology Research Institute, Yonsei University College of Medicine, Seoul, Korea. FAU - Sim, Da Woon AU - Sim da W AD - Division of Allergy and Immunology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea. AD - Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea. FAU - Park, Hye Jung AU - Park HJ AD - Division of Allergy and Immunology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea. AD - Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea. FAU - Lee, Jae Hyun AU - Lee JH AD - Division of Allergy and Immunology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea. AD - Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea. FAU - Lee, Kwang Hoon AU - Lee KH AD - Department of Dermatology & Cutaneous Biology Research Institute, Yonsei University College of Medicine, Seoul, Korea. AD - Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea. FAU - Park, Jung Won AU - Park JW AD - Division of Allergy and Immunology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea. AD - Institute of Allergy, Yonsei University College of Medicine, Seoul, Korea. parkjw@yuhs.ac. LA - eng PT - Journal Article PL - Korea (South) TA - Yonsei Med J JT - Yonsei medical journal JID - 0414003 RN - 0 (Allergens) RN - 0 (Antigens, Dermatophagoides) RN - 0 (Antigens, Neoplasm) RN - 0 (Immunoglobulin G) RN - 0 (blocking factor) RN - 37341-29-0 (Immunoglobulin E) SB - IM MH - Adult MH - Allergens/administration & dosage/*immunology MH - Animals MH - Antibody Specificity/immunology MH - Antigens, Dermatophagoides MH - *Antigens, Neoplasm MH - Dermatitis, Atopic/blood/*immunology/*therapy MH - Desensitization, Immunologic/*methods MH - Enzyme-Linked Immunosorbent Assay MH - Female MH - Humans MH - Immunoglobulin E/blood/*immunology MH - Immunoglobulin G/blood/*immunology MH - Injections, Subcutaneous MH - Male MH - Mites/*immunology MH - Pyroglyphidae/immunology MH - Treatment Outcome MH - Young Adult PMC - PMC5011275 OTO - NOTNLM OT - Blocking factor OT - IgG4 OT - house dust mite OT - immunotherapy OT - specific IgE COIS- The authors have no financial conflicts of interest. EDAT- 2016/09/07 06:00 MHDA- 2017/02/28 06:00 PMCR- 2016/11/01 CRDT- 2016/09/06 06:00 PHST- 2016/03/18 00:00 [received] PHST- 2016/05/13 00:00 [revised] PHST- 2016/05/15 00:00 [accepted] PHST- 2016/09/06 06:00 [entrez] PHST- 2016/09/07 06:00 [pubmed] PHST- 2017/02/28 06:00 [medline] PHST- 2016/11/01 00:00 [pmc-release] AID - 57.1427 [pii] AID - 10.3349/ymj.2016.57.6.1427 [doi] PST - ppublish SO - Yonsei Med J. 2016 Nov;57(6):1427-34. doi: 10.3349/ymj.2016.57.6.1427.