PMID- 27594443
OWN - NLM
STAT- MEDLINE
DCOM- 20170713
LR  - 20191210
IS  - 2008-823X (Electronic)
IS  - 1028-852X (Print)
IS  - 1028-852X (Linking)
VI  - 20
IP  - 5
DP  - 2016 Nov
TI  - Venom Components of Iranian Scorpion Hemiscorpius lepturus Inhibit the Growth and 
      Replication of Human Immunodeficiency Virus 1 (HIV-1).
PG  - 259-65
AB  - BACKGROUND: During the recent years, significant progress has been achieved on 
      development of novel anti-viral drugs. Natural products are assumed as the 
      potential sources of novel anti-viral drugs; therefore, there are some previous 
      studies reporting the anti-viral compounds from venomous animals. Based on the 
      significant value for tracing of non-toxic anti-viral agents from natural 
      resources, this study was aimed to investigate the anti-viral activity of some 
      HPLC purified fractions derived from the venom of Iranian scorpion, Hemiscorpius 
      lepturus, against human immunodeficiency virus 1 (HIV-1) and herpes simplex virus 
      1 (HSV-1). METHODS: H. Lepturus crude venom was subjected to reverse phase HPLC 
      analysis to determine its active components precisely where four dominant 
      fractions obtained at retention time of 156-160 minutes. The phospholipase A2 and 
      hemolytic activities of the purified fractions were first evaluated. Then the 
      anti-viral activity was measured using single cycle HIV (NL4-3) replication and 
      HSV (KOS) plaque reduction assays. RESULTS: The H. lepturus crude venom inhibited 
      HIV replication by 73% at the concentration of 200 microg/ml, while it did not show 
      significant anti-HSV activity. It also inhibited the cell-free viral particles in 
      a virucidal assay, while it showed no toxicity for the target cells in a 
      proliferation assay. The four HPLC fractions purified from H. lepturus inhibited 
      HIV with IC50 of 20 microg/ml. CONCLUSION: H. lepturus venom contains components with 
      considerable anti-HIV activity insofar as it has virucidal activity that offers a 
      novel therapeutic approach against HIV infection. Our results suggest a promising 
      pilot for anti-HIV drug discovery with H. lepturus scorpion venom.
FAU - Zabihollahi, Rezvan
AU  - Zabihollahi R
AD  - Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran.
FAU - Pooshang Bagheri, Kamran
AU  - Pooshang Bagheri K
AD  - Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
FAU - Keshavarz, Zohreh
AU  - Keshavarz Z
AD  - School of Nursing and Midwifery, Shahid Beheshti University of Medical Sciences, 
      Tehran, Iran.
FAU - Motevalli, Fatemeh
AU  - Motevalli F
AD  - Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran.
FAU - Bahramali, Golnaz
AU  - Bahramali G
AD  - Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran.
FAU - Siadat, Seyed Davar
AU  - Siadat SD
AD  - Department of Microbiology; Pasteur Institute of Iran; Tehran, Iran.
FAU - Momen, Seyed Bahman
AU  - Momen SB
AD  - Department of Pilot Nano-Biotechnology, Pasteur institute of Iran, Tehran, Iran.
FAU - Shahbazzadeh, Delavar
AU  - Shahbazzadeh D
AD  - Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
FAU - Aghasadeghi, Mohammad Reza
AU  - Aghasadeghi MR
AD  - Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran.
LA  - eng
PT  - Journal Article
DEP - 20160904
PL  - Iran
TA  - Iran Biomed J
JT  - Iranian biomedical journal
JID - 9814853
RN  - 0 (Antiviral Agents)
RN  - 0 (Scorpion Venoms)
RN  - EC 3.1.- (Phospholipases)
SB  - IM
MH  - Animals
MH  - Antiviral Agents/*pharmacology
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - HEK293 Cells
MH  - HIV Infections/*drug therapy
MH  - HIV-1/*growth & development
MH  - HeLa Cells
MH  - Herpes Simplex/*drug therapy
MH  - Herpesvirus 1, Human/*growth & development
MH  - Humans
MH  - Iran
MH  - Phospholipases/metabolism
MH  - Scorpion Venoms/*pharmacology
MH  - Scorpions/*pathogenicity
MH  - Vero Cells
PMC - PMC5075138
OTO - NOTNLM
OT  - Hemiscorpius lepturus
OT  - Herpes simplex virus (HSV)
OT  - Human immunodeficiency virus (HIV)
OT  - Venom
COIS- CONFLICT OF INTEREST. None declared.
EDAT- 2016/09/07 06:00
MHDA- 2017/07/14 06:00
PMCR- 2016/11/01
CRDT- 2016/09/06 06:00
PHST- 2016/09/06 06:00 [entrez]
PHST- 2016/09/07 06:00 [pubmed]
PHST- 2017/07/14 06:00 [medline]
PHST- 2016/11/01 00:00 [pmc-release]
AID - Pii-IBJ-A-10-1966 [pii]
AID - IBJ-20-259 [pii]
AID - 10.22045/ibj.2016.02 [doi]
PST - ppublish
SO  - Iran Biomed J. 2016 Nov;20(5):259-65. doi: 10.22045/ibj.2016.02. Epub 2016 Sep 4.