PMID- 27594807
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160905
LR  - 20200929
IS  - 1477-5956 (Print)
IS  - 1477-5956 (Electronic)
IS  - 1477-5956 (Linking)
VI  - 14
IP  - 1
DP  - 2016
TI  - Comparative proteome analysis of abdominal adipose tissues between fat and lean 
      broilers.
PG  - 9
LID - 10.1186/s12953-016-0100-2 [doi]
LID - 9
AB  - BACKGROUND: The molecular mechanism underlying broiler fat deposition is still 
      poorly understood. METHOD: Currently, we used two-dimensional gel electrophoresis 
      (2DE) to identify differentially expressed proteins in abdominal adipose tissues 
      of birds at 4 week of age derived from Northeast Agricultural University broiler 
      lines divergently selected for abdominal fat content (NEAUHLF). RESULTS: Thirteen 
      differentially expressed protein spots were screened out and identified by 
      matrix-assisted laser desorption-ionization time-of-flight mass spectrometry 
      (MALDI-TOF-MS). The protein spots were matched to thirteen proteins by searching 
      against the NCBInr database. These identified proteins were apolipoprotein A-I 
      (Apo A-I), cytokeratin otokeratin, ATP synthase subunit alpha, peptidyl-prolyl 
      cis-trans isomerase FKBP4 (PPIase FKBP4), aspartate aminotransferase, carbonic 
      anhydrase II (CA-II), prostaglandin-H2 D-isomerase precursor, fibrinogen alpha 
      chain, lamin-A (LMNA), superoxide dismutase [Mn] (MnSOD), heat shock protein 
      beta-1 (HSPbeta1) and two predicted proteins. These differentially expressed 
      proteins are involved mainly in lipid metabolism, amino acid metabolism, signal 
      transduction, energy conversion, antioxidant, and cytoskeleton. Differential 
      expression of Apo A-I, PPIase FKBP4, and cytokeratin otokeratin proteins were 
      further confirmed by Western blot analysis. Quantitative real-time RT-PCR 
      analyses showed that, of these 13 differentially expressed proteins, only PPIase 
      FKBP4 and cytokeratin otokeratin were differentially expressed at mRNA level 
      between the two lines. CONCLUSIONS: Our results have provided further information 
      for understanding the basic genetics control of growth and development of broiler 
      adipose tissue.
FAU - Wu, Chun-Yan
AU  - Wu CY
AD  - Key Laboratory of Chicken Genetics and Breeding of Agriculture Ministry, Key 
      Laboratory of Animal Genetics, Breeding and Reproduction of Education Department 
      of Heilongjiang Province, College of Animal Science and Technology, Northeast 
      Agricultural University, Harbin, 150030 Heilongjiang China.
FAU - Wu, Yuan-Yuan
AU  - Wu YY
AD  - Key Laboratory of Chicken Genetics and Breeding of Agriculture Ministry, Key 
      Laboratory of Animal Genetics, Breeding and Reproduction of Education Department 
      of Heilongjiang Province, College of Animal Science and Technology, Northeast 
      Agricultural University, Harbin, 150030 Heilongjiang China.
AD  - Weifang Academy of Agricultural Sciences, Weifang, 261071 Shandong China.
FAU - Liu, Chun-Dong
AU  - Liu CD
AD  - Key Laboratory of Chicken Genetics and Breeding of Agriculture Ministry, Key 
      Laboratory of Animal Genetics, Breeding and Reproduction of Education Department 
      of Heilongjiang Province, College of Animal Science and Technology, Northeast 
      Agricultural University, Harbin, 150030 Heilongjiang China.
FAU - Wang, Yu-Xiang
AU  - Wang YX
AD  - Key Laboratory of Chicken Genetics and Breeding of Agriculture Ministry, Key 
      Laboratory of Animal Genetics, Breeding and Reproduction of Education Department 
      of Heilongjiang Province, College of Animal Science and Technology, Northeast 
      Agricultural University, Harbin, 150030 Heilongjiang China.
FAU - Na, Wei
AU  - Na W
AD  - Key Laboratory of Chicken Genetics and Breeding of Agriculture Ministry, Key 
      Laboratory of Animal Genetics, Breeding and Reproduction of Education Department 
      of Heilongjiang Province, College of Animal Science and Technology, Northeast 
      Agricultural University, Harbin, 150030 Heilongjiang China.
FAU - Wang, Ning
AU  - Wang N
AD  - Key Laboratory of Chicken Genetics and Breeding of Agriculture Ministry, Key 
      Laboratory of Animal Genetics, Breeding and Reproduction of Education Department 
      of Heilongjiang Province, College of Animal Science and Technology, Northeast 
      Agricultural University, Harbin, 150030 Heilongjiang China.
FAU - Li, Hui
AU  - Li H
AD  - Key Laboratory of Chicken Genetics and Breeding of Agriculture Ministry, Key 
      Laboratory of Animal Genetics, Breeding and Reproduction of Education Department 
      of Heilongjiang Province, College of Animal Science and Technology, Northeast 
      Agricultural University, Harbin, 150030 Heilongjiang China.
LA  - eng
PT  - Journal Article
DEP - 20160901
PL  - England
TA  - Proteome Sci
JT  - Proteome science
JID - 101170539
PMC - PMC5009711
OTO - NOTNLM
OT  - Abdominal adipose tissue
OT  - Broiler
OT  - Differentially expressed protein
OT  - Proteomics
EDAT- 2016/09/07 06:00
MHDA- 2016/09/07 06:01
PMCR- 2016/09/01
CRDT- 2016/09/06 06:00
PHST- 2016/01/27 00:00 [received]
PHST- 2016/08/16 00:00 [accepted]
PHST- 2016/09/06 06:00 [entrez]
PHST- 2016/09/07 06:00 [pubmed]
PHST- 2016/09/07 06:01 [medline]
PHST- 2016/09/01 00:00 [pmc-release]
AID - 100 [pii]
AID - 10.1186/s12953-016-0100-2 [doi]
PST - epublish
SO  - Proteome Sci. 2016 Sep 1;14(1):9. doi: 10.1186/s12953-016-0100-2. eCollection 
      2016.