PMID- 27595774
OWN - NLM
STAT- MEDLINE
DCOM- 20180423
LR  - 20180423
IS  - 1439-0272 (Electronic)
IS  - 0303-4569 (Linking)
VI  - 49
IP  - 6
DP  - 2017 Aug
TI  - Effect of semen preparation technique and its incubation on sperm quality in the 
      Moroccan population.
LID - 10.1111/and.12688 [doi]
AB  - In in vitro fertilisation (IVF), sperm preparation as critical part and 
      influencing the sperm quality is especially dependent on the chosen technique 
      itself and incubation parameters including temperature and CO2. In this study, we 
      compared firstly density-gradient centrifugation technique (DGC) to the adapted 
      DGC using the sperm pellet of 80% fraction (DGC/80P) in order to improve the 
      sperm yield. Secondly, this study led to evaluate different sperm incubation 
      conditions based on temperature effect (room temperature (RT = 23 degrees C) versus 35 degrees C) 
      and in the other hand, with or without 5% CO2 during 24 hrs. Based on evaluating 
      sperm conventional parameters and the DNA damage using TUNEL assay, our result 
      showed that DGC/80P increased sperm quality compared to DGC with 25% of 
      improvement. For temperature incubation effect after 24 hrs, 35 degrees C increased the 
      DNA damage and decreased the sperm quality while RT could improve sperm motility 
      by 38%. Moreover, the sperm incubation with 5% CO2 after 24 hrs realised a 
      negative impact on sperm parameters and its DNA damage. Indeed, for current IVF 
      practice, a good sperm quality can be maintained for several hours at room 
      temperature, while the sperm preparation is processed using the DGC/80P without 
      CO2.
CI  - (c) 2016 Blackwell Verlag GmbH.
FAU - Aboulmaouahib, S
AU  - Aboulmaouahib S
AD  - Laboratoire de Physiologie et Genetique Moleculaire (PGM), Departement de 
      Biologie, Faculte des Sciences Ain Chock, Universite HASSAN II, Casablanca, 
      Morocco.
AD  - Labomac IVF Centers and Clinical Laboratory Medicine, Casablanca, Morocco.
FAU - Madkour, A
AU  - Madkour A
AD  - Labomac IVF Centers and Clinical Laboratory Medicine, Casablanca, Morocco.
AD  - Biochemistry and Immunology Laboratory, Faculty of Sciences, Mohammed V 
      University, Rabat, Morocco.
AD  - Place de Nid Aux Iris, Clinique des Iris, IVF Center IRIFIV, Casablanca, Morocco.
AD  - Private Clinic of Human Reproduction and Endoscopic Surgery, Anfa Fertility 
      Center, Casablanca, Morroco.
FAU - Kaarouch, I
AU  - Kaarouch I
AD  - Labomac IVF Centers and Clinical Laboratory Medicine, Casablanca, Morocco.
AD  - Biochemistry and Immunology Laboratory, Faculty of Sciences, Mohammed V 
      University, Rabat, Morocco.
AD  - Place de Nid Aux Iris, Clinique des Iris, IVF Center IRIFIV, Casablanca, Morocco.
AD  - Private Clinic of Human Reproduction and Endoscopic Surgery, Anfa Fertility 
      Center, Casablanca, Morroco.
FAU - Saadani, B
AU  - Saadani B
AD  - Place de Nid Aux Iris, Clinique des Iris, IVF Center IRIFIV, Casablanca, Morocco.
FAU - Sefrioui, O
AU  - Sefrioui O
AD  - Private Clinic of Human Reproduction and Endoscopic Surgery, Anfa Fertility 
      Center, Casablanca, Morroco.
FAU - Louanjli, N
AU  - Louanjli N
AD  - Labomac IVF Centers and Clinical Laboratory Medicine, Casablanca, Morocco.
FAU - Copin, H
AU  - Copin H
AD  - Reproductive Biology and Medical Cytogenetics Laboratory, Regional University 
      Hospital & School of Medicine, Picardie University Jules Verne, Amiens, France.
FAU - Cadi, R
AU  - Cadi R
AD  - Laboratoire de Physiologie et Genetique Moleculaire (PGM), Departement de 
      Biologie, Faculte des Sciences Ain Chock, Universite HASSAN II, Casablanca, 
      Morocco.
FAU - Benkhalifa, M
AU  - Benkhalifa M
AD  - Reproductive Biology and Medical Cytogenetics Laboratory, Regional University 
      Hospital & School of Medicine, Picardie University Jules Verne, Amiens, France.
LA  - eng
PT  - Journal Article
DEP - 20160905
PL  - Germany
TA  - Andrologia
JT  - Andrologia
JID - 0423506
SB  - IM
MH  - Centrifugation, Density Gradient
MH  - *DNA Damage
MH  - *DNA Fragmentation
MH  - Fertilization in Vitro
MH  - Humans
MH  - Male
MH  - Morocco
MH  - Semen Analysis
MH  - Semen Preservation/*methods
MH  - Sperm Motility
MH  - *Spermatozoa
OTO - NOTNLM
OT  - DNA decays
OT  - parameter improvement
OT  - semen preparation
EDAT- 2016/09/07 06:00
MHDA- 2018/04/24 06:00
CRDT- 2016/09/07 06:00
PHST- 2016/06/25 00:00 [accepted]
PHST- 2016/09/07 06:00 [pubmed]
PHST- 2018/04/24 06:00 [medline]
PHST- 2016/09/07 06:00 [entrez]
AID - 10.1111/and.12688 [doi]
PST - ppublish
SO  - Andrologia. 2017 Aug;49(6). doi: 10.1111/and.12688. Epub 2016 Sep 5.