PMID- 27596279
OWN - NLM
STAT- MEDLINE
DCOM- 20171205
LR  - 20171213
IS  - 1872-7603 (Electronic)
IS  - 0165-0378 (Linking)
VI  - 118
DP  - 2016 Nov
TI  - Longitudinal characterization of bovine monocyte-derived dendritic cells from 
      mid-gestation into subsequent lactation reveals nadir in phenotypic maturation 
      and macrophage-like cytokine profile in late gestation.
PG  - 1-8
LID - S0165-0378(16)30094-8 [pii]
LID - 10.1016/j.jri.2016.08.003 [doi]
AB  - Changes in monocyte and dendritic cell populations during bovine pregnancy and 
      lactation remain poorly described despite the key roles these cells play in 
      immune tolerance and activation. Using a prospective longitudinal study, we 
      characterized CD14+ monocyte-derived dendritic cell (moDC) differentiation and 
      maturation and captured monocyte composition dynamics from mid-gestation through 
      calving and into the subsequent lactation in dairy cows (n=7). First, we measured 
      absolute counts of classical (CD14+CD16-, cM), intermediate (CD14+CD16+, intM), 
      and nonclassical (CD14-CD16+, ncM) monocytes in the blood and determined 
      proportions of individual subsets within the total monocyte population. We found 
      the proportion of cM decreased and intM increased significantly by early 
      lactation, whereas there was a nadir in the proportion of ncM in late gestation, 
      two weeks prepartum. Monocyte composition appears to be regulated in pregnancy, 
      possibly to limit the proportion of highly inflammatory monocytes i.e. intM. 
      Ultimately, we found that moDC differentiated from CD14+ monocytes isolated in 
      the early dry period of late gestation had impaired E. coli-induced maturation, 
      with nadirs in upregulation of CD80 and MHC II, and downregulation of CD14. The 
      moDC from late gestation also had altered cytokine profiles with greatest 
      production of pro-inflammatory IL-1beta and anti-inflammatory IL-10. These data 
      suggest monocytes in late gestation, in contrast to other stages of pregnancy and 
      lactation, differentiate and maturate into moDC less capable of eliciting strong 
      T cell activation, and have macrophage-like cytokine profiles. These results 
      provide insight into maternal immune modulation and elucidate potential immune 
      changes necessary to facilitate bovine pregnancy.
CI  - Copyright (c) 2016 Elsevier Ireland Ltd. All rights reserved.
FAU - Pomeroy, Brianna
AU  - Pomeroy B
AD  - S3 119, Schurman Hall, College of Veterinary Medicine, Cornell University, 
      Ithaca, NY 14850, United States; Department of Population Medicine & Diagnostic 
      Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, 
      United States. Electronic address: bjp62@cornell.edu.
FAU - Sipka, Anja
AU  - Sipka A
AD  - Department of Population Medicine & Diagnostic Sciences, College of Veterinary 
      Medicine, Cornell University, Ithaca, NY 14853, United States. Electronic 
      address: ass233@cornell.edu.
FAU - Klaessig, Suzanne
AU  - Klaessig S
AD  - Department of Population Medicine & Diagnostic Sciences, College of Veterinary 
      Medicine, Cornell University, Ithaca, NY 14853, United States. Electronic 
      address: skc10@cornell.edu.
FAU - Schukken, Ynte
AU  - Schukken Y
AD  - Department of Population Medicine & Diagnostic Sciences, College of Veterinary 
      Medicine, Cornell University, Ithaca, NY 14853, United States; GD Animal Health, 
      Deventer, The Netherlands; Department of Animal Sciences, Wageningen University, 
      Wageningen, The Netherlands. Electronic address: Y.Schukken@gddeventer.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20160830
PL  - Ireland
TA  - J Reprod Immunol
JT  - Journal of reproductive immunology
JID - 8001906
RN  - 0 (Interleukin-1beta)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Receptors, IgG)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Immune Tolerance
MH  - Immunophenotyping
MH  - Interleukin-10/metabolism
MH  - Interleukin-1beta/metabolism
MH  - Lactation/immunology
MH  - Lipopolysaccharide Receptors/metabolism
MH  - Longitudinal Studies
MH  - Lymphocyte Activation
MH  - Macrophages/*immunology
MH  - Monocytes/*immunology
MH  - Pregnancy/*immunology
MH  - Prospective Studies
MH  - Receptors, IgG/metabolism
MH  - T-Lymphocytes/*immunology
OTO - NOTNLM
OT  - Bovine
OT  - Monocyte-derived dendritic cells
OT  - Monocytes
OT  - Pregnancy
EDAT- 2016/09/07 06:00
MHDA- 2017/12/06 06:00
CRDT- 2016/09/07 06:00
PHST- 2016/04/08 00:00 [received]
PHST- 2016/07/25 00:00 [revised]
PHST- 2016/08/15 00:00 [accepted]
PHST- 2016/09/07 06:00 [pubmed]
PHST- 2017/12/06 06:00 [medline]
PHST- 2016/09/07 06:00 [entrez]
AID - S0165-0378(16)30094-8 [pii]
AID - 10.1016/j.jri.2016.08.003 [doi]
PST - ppublish
SO  - J Reprod Immunol. 2016 Nov;118:1-8. doi: 10.1016/j.jri.2016.08.003. Epub 2016 Aug 
      30.