PMID- 27597287
OWN - NLM
STAT- MEDLINE
DCOM- 20171017
LR  - 20190221
IS  - 1872-8332 (Electronic)
IS  - 0169-5002 (Linking)
VI  - 100
DP  - 2016 Oct
TI  - Immunohistochemical pattern analysis of squamous cell carcinoma: Lung primary and 
      metastatic tumors of head and neck.
PG  - 96-101
LID - S0169-5002(16)30444-5 [pii]
LID - 10.1016/j.lungcan.2016.08.003 [doi]
AB  - OBJECTIVES: This study aimed to develop an immunohistochemical (IHC) diagnostic 
      algorithm for primary lung squamous cell carcinoma (LSCC) and pulmonary 
      metastasis of head and neck SCC (HNSCC). MATERIALS AND METHODS: We selected three 
      antibodies (CK19, MMP3, and PI3) from a web-based gene expression database and an 
      IHC analysis available online. We developed an IHC diagnostic algorithm using 
      tissue microarrays from 39 LSCCs and 48 HNSCCs as the training set. It was 
      validated using whole tumor sections of 32 LSCCs and 23 HNSCCs. The algorithm was 
      applied to 28 cases with a history of HNSCC and who underwent resection of 
      pulmonary squamous cell tumors. RESULTS: The sensitivity, specificity, and 
      accuracy of the algorithm were 90%, 62%, and 77%, respectively, in the training 
      set and 96%, 44%, and 65%, respectively, in the validation set. Twenty-three of 
      28 SCCs were diagnosed as metastasis of HNSCC; the remaining five tumors were 
      diagnosed as LSCC. Among the patients in the HNSCC group, 18 developed 
      postoperative recurrence and 11 died of the disease, whereas only one patient in 
      the LSCC group had recurrence. Compared with the LSCC group, the HNSCC group had 
      poorer prognosis (P=0.07). IHC diagnosis coincided with the retrospective 
      diagnosis in 22 (79%) of the 28 patients (sensitivity, 95%; specificity, 44%). 
      CONCLUSION: The IHC diagnostic algorithm may be clinically useful for 
      distinguishing between LSCC and pulmonary metastasis of HNSCC.
CI  - Copyright (c) 2016 Elsevier Ireland Ltd. All rights reserved.
FAU - Ichinose, Junji
AU  - Ichinose J
AD  - Department of Thoracic Surgery, University of Tokyo Hospital, Tokyo, Japan. 
      Electronic address: jichinose-tky@umin.ac.jp.
FAU - Shinozaki-Ushiku, Aya
AU  - Shinozaki-Ushiku A
AD  - Department of Pathology, University of Tokyo Hospital, Tokyo, Japan.
FAU - Nagayama, Kazuhiro
AU  - Nagayama K
AD  - Department of Thoracic Surgery, University of Tokyo Hospital, Tokyo, Japan.
FAU - Nitadori, Jun-Ichi
AU  - Nitadori JI
AD  - Department of Thoracic Surgery, University of Tokyo Hospital, Tokyo, Japan.
FAU - Anraku, Masaki
AU  - Anraku M
AD  - Department of Thoracic Surgery, University of Tokyo Hospital, Tokyo, Japan.
FAU - Fukayama, Masashi
AU  - Fukayama M
AD  - Department of Pathology, University of Tokyo Hospital, Tokyo, Japan.
FAU - Nakajima, Jun
AU  - Nakajima J
AD  - Department of Thoracic Surgery, University of Tokyo Hospital, Tokyo, Japan.
FAU - Takai, Daiya
AU  - Takai D
AD  - Department of Clinical Laboratory, University of Tokyo Hospital, Tokyo, Japan.
LA  - eng
PT  - Journal Article
DEP - 20160810
PL  - Ireland
TA  - Lung Cancer
JT  - Lung cancer (Amsterdam, Netherlands)
JID - 8800805
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Algorithms
MH  - Carcinoma, Squamous Cell/*metabolism/pathology/secondary/surgery
MH  - Diagnostic Techniques and Procedures
MH  - Female
MH  - Head and Neck Neoplasms/metabolism/pathology/*secondary
MH  - Humans
MH  - Immunohistochemistry/*methods
MH  - Lung Neoplasms/*metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Recurrence, Local/metabolism/pathology
MH  - Predictive Value of Tests
MH  - Retrospective Studies
MH  - Squamous Cell Carcinoma of Head and Neck
OTO - NOTNLM
OT  - Diagnosis
OT  - Immunohistochemistry
OT  - Lung cancer
OT  - Pulmonary metastasis
OT  - Squamous cell carcinoma
EDAT- 2016/09/07 06:00
MHDA- 2017/10/19 06:00
CRDT- 2016/09/07 06:00
PHST- 2016/06/22 00:00 [received]
PHST- 2016/08/01 00:00 [revised]
PHST- 2016/08/09 00:00 [accepted]
PHST- 2016/09/07 06:00 [entrez]
PHST- 2016/09/07 06:00 [pubmed]
PHST- 2017/10/19 06:00 [medline]
AID - S0169-5002(16)30444-5 [pii]
AID - 10.1016/j.lungcan.2016.08.003 [doi]
PST - ppublish
SO  - Lung Cancer. 2016 Oct;100:96-101. doi: 10.1016/j.lungcan.2016.08.003. Epub 2016 
      Aug 10.