PMID- 27600145 OWN - NLM STAT- MEDLINE DCOM- 20170102 LR - 20181202 IS - 1129-2377 (Electronic) IS - 1129-2369 (Print) IS - 1129-2369 (Linking) VI - 17 IP - 1 DP - 2016 Dec TI - Expression of artemin and GFRalpha3 in an animal model of migraine: possible role in the pathogenesis of this disorder. PG - 81 LID - 10.1186/s10194-016-0673-2 [doi] LID - 81 AB - BACKGROUND: Neurotrophic factors have been implicated in hyperalgesia and peripheral levels of these molecules are altered in migraine pathophysiology. Artemin, a vasculature-derived neurotrophic factor, contributes to pain modulation and trigeminal primary afferent sensitization through binding its selective receptor GFRalpha3. The distribution of artemin and GFRalpha3 in the dura mater raises an anatomy supports that they may be involved in migraine. In this study we evaluated the expression of artemin and GFRalpha3 in an animal migraine model that may be relevant for migraine. METHODS: In this study, using a rat migraine model by administration of nitroglycerin (NTG), we investigated the expression of artemin in the dura mater and GFRalpha3 in the trigeminal ganglia (TG) by means of quantitative reverse transcription-polymerase chain reaction, western blot and immunofluorescence labeling. RESULTS: Artemin immunoreactivity was found in the smooth muscle cells of dural vasculature and GFRalpha3 was present in cytoplasm of TG neurons. The mRNA levels of artemin and GFRalpha3 were significantly elevated after NTG treatment at 2 and 4 h respectively (P < 0.05). The expression of artemin protein was increased at 4 h and continually up to 8 h in the dura mater following NTG administration (P < 0.05). The expression of GFRalpha3 protein was elevated at 4 h and continually up to 10 h in the TG following NTG administration (P < 0.05). CONCLUSION: The findings suggest that artemin and GFRalpha3 play an important role in the pathogenesis of migraine and may represent potential therapeutic targets for the treatment of migraine. FAU - Shang, Hai-Qiong AU - Shang HQ AD - Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250021, People's Republic of China. AD - Shandong Provincial Key Laboratory of Otology, Jinan, 250022, People's Republic of China. FAU - Wang, Yan AU - Wang Y AD - Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250021, People's Republic of China. AD - Department of Otolaryngology, People's Hospital of Rizhao, Rizhao, 276800, People's Republic of China. FAU - Mao, Yan-Yan AU - Mao YY AD - Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250021, People's Republic of China. AD - Shandong Provincial Key Laboratory of Otology, Jinan, 250022, People's Republic of China. FAU - Kong, Li-Gang AU - Kong LG AD - Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250021, People's Republic of China. AD - Shandong Provincial Key Laboratory of Otology, Jinan, 250022, People's Republic of China. FAU - Sun, Gao-Ying AU - Sun GY AD - Shandong Provincial Key Laboratory of Otology, Jinan, 250022, People's Republic of China. AD - Institute of Eye and ENT, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250022, People's Republic of China. FAU - Xu, Lei AU - Xu L AD - Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250021, People's Republic of China. FAU - Zhang, Dao-Gong AU - Zhang DG AD - Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250021, People's Republic of China. FAU - Han, Yue-Chen AU - Han YC AD - Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250021, People's Republic of China. FAU - Li, Jian-Feng AU - Li JF AD - Shandong Provincial Key Laboratory of Otology, Jinan, 250022, People's Republic of China. AD - Institute of Eye and ENT, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250022, People's Republic of China. FAU - Wang, Hai-Bo AU - Wang HB AD - Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250021, People's Republic of China. AD - Shandong Provincial Key Laboratory of Otology, Jinan, 250022, People's Republic of China. AD - Institute of Eye and ENT, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250022, People's Republic of China. FAU - Fan, Zhao-Min AU - Fan ZM AD - Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, 250021, People's Republic of China. fanzment@outlook.com. LA - eng PT - Journal Article DEP - 20160906 PL - England TA - J Headache Pain JT - The journal of headache and pain JID - 100940562 RN - 0 (Artn protein, rat) RN - 0 (Gfra3 protein, rat) RN - 0 (Glial Cell Line-Derived Neurotrophic Factor Receptors) RN - 0 (Nerve Tissue Proteins) SB - IM MH - Animals MH - Disease Models, Animal MH - Dura Mater/*metabolism MH - Glial Cell Line-Derived Neurotrophic Factor Receptors/*metabolism MH - Male MH - Migraine Disorders/chemically induced/*metabolism MH - Nerve Tissue Proteins/*metabolism MH - Rats MH - Rats, Wistar MH - Trigeminal Ganglion/*metabolism PMC - PMC5013005 OTO - NOTNLM OT - Artemin OT - Dura mater OT - GFRalpha3 OT - Migraine OT - Trigeminal ganglia EDAT- 2016/09/08 06:00 MHDA- 2017/01/04 06:00 PMCR- 2016/09/06 CRDT- 2016/09/08 06:00 PHST- 2016/05/14 00:00 [received] PHST- 2016/09/01 00:00 [accepted] PHST- 2016/09/08 06:00 [entrez] PHST- 2016/09/08 06:00 [pubmed] PHST- 2017/01/04 06:00 [medline] PHST- 2016/09/06 00:00 [pmc-release] AID - 10.1186/s10194-016-0673-2 [pii] AID - 673 [pii] AID - 10.1186/s10194-016-0673-2 [doi] PST - ppublish SO - J Headache Pain. 2016 Dec;17(1):81. doi: 10.1186/s10194-016-0673-2. Epub 2016 Sep 6.