PMID- 27600506
OWN - NLM
STAT- MEDLINE
DCOM- 20170518
LR  - 20201215
IS  - 1098-5522 (Electronic)
IS  - 0019-9567 (Print)
IS  - 0019-9567 (Linking)
VI  - 84
IP  - 12
DP  - 2016 Dec
TI  - MicroRNAs Regulate Cytokine Responses in Gingival Epithelial Cells.
PG  - 3282-3289
AB  - MicroRNAs (miRNAs) have been established as key regulators of various biological 
      processes with possible involvement in the pathobiology of periodontal disease. 
      Expanding our earlier observations of substantial differential expression of 
      specific miRNAs between clinically healthy and periodontitis-affected gingival 
      tissues, we used miRNA inhibitors (sponges) in loss-of-function experiments to 
      investigate the involvement of specific miRNAs in the response of pocket 
      epithelium-derived, telomerase-immortalized human gingival keratinocytes (TIGKs) 
      to microbial infection. We constructed stable knockdown (KD) cell lines for five 
      epithelium-expressed miRNAs (miR-126, miR-141, miR-155, miR-210, and miR-1246) 
      and assessed their response to infection with periodontal pathogens using 
      microarray analysis, quantitative PCR (qPCR), enzyme-linked immunosorbent assay 
      (ELISA), and Western blot assay. miR-126 KD cells showed lower expression of 
      interleukin 8 (IL-8) and CXCL1, both on the mRNA and protein levels, than did 
      controls upon stimulation by heat-killed wild-type Porphyromonas gingivalis, live 
      P. gingivalis protease-deficient mutant KDP128, and live Aggregatibacter 
      actinomycetemcomitans In contrast, infection of miR-155 KD and miR-210 KD cells 
      with the same organisms resulted in higher IL-8 and CXCL1 mRNA and protein 
      expression. These effects appeared to be regulated by NF-kappaB, as suggested by 
      altered transcription and/or phosphorylation status of components of the NF-kappaB 
      system. Reduced neutrophil-like HL-60 cell chemotactic activity was observed in 
      response to infection of miR-126 KD cells, indicating that miR-126 plays an 
      important role in immune responses. Our findings indicate that specific miRNAs 
      regulate the expression of inflammatory cytokines in human gingival epithelial 
      cells in response to microbial infection.
CI  - Copyright (c) 2016, American Society for Microbiology. All Rights Reserved.
FAU - Chen, Steven C Y
AU  - Chen SC
AD  - Division of Periodontics, Section of Oral, Diagnostic and Rehabilitations 
      Sciences, College of Dental Medicine, Columbia University, New York, New York, 
      USA.
FAU - Constantinides, Christos
AU  - Constantinides C
AD  - Division of Periodontics, Section of Oral, Diagnostic and Rehabilitations 
      Sciences, College of Dental Medicine, Columbia University, New York, New York, 
      USA.
FAU - Kebschull, Moritz
AU  - Kebschull M
AD  - Division of Periodontics, Section of Oral, Diagnostic and Rehabilitations 
      Sciences, College of Dental Medicine, Columbia University, New York, New York, 
      USA.
AD  - Department of Periodontology, Operative and Preventive Dentistry, University of 
      Bonn, Bonn, Germany.
FAU - Papapanou, Panos N
AU  - Papapanou PN
AD  - Division of Periodontics, Section of Oral, Diagnostic and Rehabilitations 
      Sciences, College of Dental Medicine, Columbia University, New York, New York, 
      USA pp192@cumc.columbia.edu.
LA  - eng
GR  - R03 DE024735/DE/NIDCR NIH HHS/United States
GR  - S10 OD020056/OD/NIH HHS/United States
GR  - UL1 TR000040/TR/NCATS NIH HHS/United States
GR  - R01 DE015649/DE/NIDCR NIH HHS/United States
GR  - R21 DE021820/DE/NIDCR NIH HHS/United States
PT  - Journal Article
DEP - 20161118
PL  - United States
TA  - Infect Immun
JT  - Infection and immunity
JID - 0246127
RN  - 0 (Cytokines)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Aggregatibacter actinomycetemcomitans
MH  - Cell Line
MH  - Chemotaxis
MH  - Cytokines/genetics/*metabolism
MH  - Gene Expression Regulation/*immunology
MH  - Gene Knockdown Techniques
MH  - Gingiva/*cytology
MH  - HL-60 Cells
MH  - Humans
MH  - Keratinocytes/*metabolism
MH  - MicroRNAs/genetics/*metabolism
MH  - Porphyromonas gingivalis
PMC - PMC5116707
EDAT- 2016/09/08 06:00
MHDA- 2017/05/19 06:00
PMCR- 2017/05/18
CRDT- 2016/09/08 06:00
PHST- 2016/03/26 00:00 [received]
PHST- 2016/08/30 00:00 [accepted]
PHST- 2016/09/08 06:00 [pubmed]
PHST- 2017/05/19 06:00 [medline]
PHST- 2016/09/08 06:00 [entrez]
PHST- 2017/05/18 00:00 [pmc-release]
AID - IAI.00263-16 [pii]
AID - 00263-16 [pii]
AID - 10.1128/IAI.00263-16 [doi]
PST - epublish
SO  - Infect Immun. 2016 Nov 18;84(12):3282-3289. doi: 10.1128/IAI.00263-16. Print 2016 
      Dec.