PMID- 27600807
OWN - NLM
STAT- MEDLINE
DCOM- 20170215
LR  - 20181113
IS  - 1432-2307 (Electronic)
IS  - 0945-6317 (Linking)
VI  - 469
IP  - 5
DP  - 2016 Nov
TI  - Cellular-level characterization of B cells infiltrating pulmonary MALT lymphoma 
      tissues.
PG  - 575-580
AB  - Mucosa-associated lymphoid tissue (MALT) lymphoma mainly consists of three types 
      of tumor B cells, small (centrocyte-like), scattered large transformed, and 
      intraepithelial. However, it is difficult to differentiate tumor B cells from 
      reactive B cells at the cellular level. We examined five cases of API2-MALT1 
      fusion-positive MALT lymphoma of the lung. A single paraffin section for each 
      case was subjected to sequential retrieval of whole-slide imaging (WSI) data of 
      hematoxylin and eosin (HE) staining, immunofluorescence staining for CD79a, and 
      fluorescence in situ hybridization (FISH) for the MALT1 split. We counted the 
      number of MALT1 split-positive or MALT1 split-negative cells among CD79a-positive 
      cells. The MALT1 split was detected in 59, 46, and 76 % of small, large, and 
      intraepithelial B cells, respectively. A review of the HE-WSI data showed that 
      cytomorphological distinction between the MALT1 split-positive and MALT1 
      split-negative B cells was virtually impossible. None of CD79a-negative lymphoid 
      cells, epithelial cells, and microvascular endothelial cells was positive for 
      MALT1 splits. As API2-MALT1 fusion is an early and critical event in the 
      lymphomatogenesis, our findings are best interpreted as that a considerable 
      number of B cells, either small, large, or intraepithelial, are reactive cells 
      and that it is difficult to distinguish cytomorphologically between tumor B cells 
      and reactive B cells. These findings suggest that the tumor architecture may be 
      the central factor for making a correct histopathological diagnosis of MALT 
      lymphoma. The sequential WSI of HE staining, immunofluorescence staining, and 
      FISH as described here is a useful tool for pathological analysis at the cellular 
      level.
FAU - Fujii, Keiichiro
AU  - Fujii K
AD  - Department of Pathology and Molecular Diagnostics, Graduate School of Medical 
      Sciences, Nagoya City University, 1 Kawasumi Mizuho-cho, Mizuho-ku, Nagoya, 
      467-8601, Japan.
FAU - Ishibashi, Ken-Ichiro
AU  - Ishibashi KI
AD  - Department of Pathology and Molecular Diagnostics, Graduate School of Medical 
      Sciences, Nagoya City University, 1 Kawasumi Mizuho-cho, Mizuho-ku, Nagoya, 
      467-8601, Japan.
FAU - Kato, Junki
AU  - Kato J
AD  - Department of Pathology and Molecular Diagnostics, Graduate School of Medical 
      Sciences, Nagoya City University, 1 Kawasumi Mizuho-cho, Mizuho-ku, Nagoya, 
      467-8601, Japan.
FAU - Kan, Jushin
AU  - Kan J
AD  - Department of Pathology and Molecular Diagnostics, Graduate School of Medical 
      Sciences, Nagoya City University, 1 Kawasumi Mizuho-cho, Mizuho-ku, Nagoya, 
      467-8601, Japan.
FAU - Fujii, Kana
AU  - Fujii K
AD  - Department of Pathology and Molecular Diagnostics, Graduate School of Medical 
      Sciences, Nagoya City University, 1 Kawasumi Mizuho-cho, Mizuho-ku, Nagoya, 
      467-8601, Japan.
FAU - Ito, Yohei
AU  - Ito Y
AD  - Department of Pathology and Molecular Diagnostics, Graduate School of Medical 
      Sciences, Nagoya City University, 1 Kawasumi Mizuho-cho, Mizuho-ku, Nagoya, 
      467-8601, Japan.
FAU - Takino, Hisashi
AU  - Takino H
AD  - Department of Pathology and Molecular Diagnostics, Graduate School of Medical 
      Sciences, Nagoya City University, 1 Kawasumi Mizuho-cho, Mizuho-ku, Nagoya, 
      467-8601, Japan.
FAU - Masaki, Ayako
AU  - Masaki A
AD  - Department of Pathology and Molecular Diagnostics, Graduate School of Medical 
      Sciences, Nagoya City University, 1 Kawasumi Mizuho-cho, Mizuho-ku, Nagoya, 
      467-8601, Japan.
FAU - Murase, Takayuki
AU  - Murase T
AD  - Department of Pathology and Molecular Diagnostics, Graduate School of Medical 
      Sciences, Nagoya City University, 1 Kawasumi Mizuho-cho, Mizuho-ku, Nagoya, 
      467-8601, Japan.
FAU - Inagaki, Hiroshi
AU  - Inagaki H
AUID- ORCID: 0000-0001-6157-636X
AD  - Department of Pathology and Molecular Diagnostics, Graduate School of Medical 
      Sciences, Nagoya City University, 1 Kawasumi Mizuho-cho, Mizuho-ku, Nagoya, 
      467-8601, Japan. hinagaki@med.nagoya-cu.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20160906
PL  - Germany
TA  - Virchows Arch
JT  - Virchows Archiv : an international journal of pathology
JID - 9423843
RN  - 0 (CD79 Antigens)
RN  - 0 (CD79A protein, human)
RN  - 0 (Oncogene Proteins, Fusion)
SB  - IM
MH  - B-Lymphocytes/*pathology
MH  - CD79 Antigens/metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Lung Neoplasms/diagnosis/*pathology
MH  - Lymphoma, B-Cell, Marginal Zone/diagnosis/*pathology
MH  - Oncogene Proteins, Fusion/genetics
MH  - Translocation, Genetic/genetics
OTO - NOTNLM
OT  - API2-MALT1 fusion
OT  - Fluorescence in situ hybridization
OT  - Immunofluorescence
OT  - MALT lymphoma
OT  - Microenvironment
OT  - Whole-slide imaging
EDAT- 2016/10/28 06:00
MHDA- 2017/02/16 06:00
CRDT- 2016/09/08 06:00
PHST- 2016/05/09 00:00 [received]
PHST- 2016/08/29 00:00 [accepted]
PHST- 2016/07/24 00:00 [revised]
PHST- 2016/10/28 06:00 [pubmed]
PHST- 2017/02/16 06:00 [medline]
PHST- 2016/09/08 06:00 [entrez]
AID - 10.1007/s00428-016-2012-z [pii]
AID - 10.1007/s00428-016-2012-z [doi]
PST - ppublish
SO  - Virchows Arch. 2016 Nov;469(5):575-580. doi: 10.1007/s00428-016-2012-z. Epub 2016 
      Sep 6.