PMID- 27601175
OWN - NLM
STAT- MEDLINE
DCOM- 20170605
LR  - 20181113
IS  - 1875-8355 (Electronic)
IS  - 1572-3887 (Linking)
VI  - 35
IP  - 5
DP  - 2016 Oct
TI  - Multi-modal Binding of a 'Self' Peptide by HLA-B*27:04 and B*27:05 Allelic 
      Variants, but not B*27:09 or B*27:06 Variants: Fresh Support for Some Theories 
      Explaining Differential Disease Association.
PG  - 346-353
AB  - A self-derived-peptide with the same amino acid sequence (N-RRYLENGKETLQR-C) as 
      residues 169-181 of the human leukocyte antigen (HLA) B27 heavy chain is known to 
      bind to MHC Class I complexes containing the HLA-B27 heavy chain. This 
      observation has been invoked previously in at least two different (but related) 
      molecular explanations for the disease-association of the HLA-B27 allele. Here, 
      we use a combination of fluorescence polarization, competitive inhibition and gel 
      filtration chromatographic studies to show that a fluorescently-labeled peptide 
      of the above sequence binds to two disease-associated subtypes of HLA-B27 (namely 
      HLA-B*27:04 and HLA-B*27:05) but not to non-disease-associated subtypes 
      (HLA-B*27:06 or HLA-B*27:09). This differential binding behavior is seen both in 
      (a) peptide binding to complexes of heavy chain (HLA-B27) and light chain (beta(2) 
      microglobulin), and in (b) peptide binding to beta(2) microglobulin-free heavy 
      chains in the aggregated state. Such subtype-specific differences are not seen 
      with two other control peptides known to bind to HLA-B27. Our results support the 
      likelihood of differential peptide binding holding at least one of the keys to 
      HLA-B27's disease association.
FAU - Rana, Manish Kumar
AU  - Rana MK
AD  - Department of Immunopathology, Research Block A, Postgraduate Institute of 
      Medical Education and Research (PGIMER), Sector-12, Chandigarh, 160012, India.
FAU - Luthra-Guptasarma, Manni
AU  - Luthra-Guptasarma M
AD  - Department of Immunopathology, Research Block A, Postgraduate Institute of 
      Medical Education and Research (PGIMER), Sector-12, Chandigarh, 160012, India. 
      mguptasarma@yahoo.com.
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Protein J
JT  - The protein journal
JID - 101212092
RN  - 0 (Fluorescent Dyes)
RN  - 0 (HLA-B*27:04 antigen)
RN  - 0 (HLA-B*27:05 antigen)
RN  - 0 (HLA-B*27:06 antigen)
RN  - 0 (HLA-B*27:09 antigen)
RN  - 0 (HLA-B27 Antigen)
RN  - 0 (Peptides)
RN  - 0 (Recombinant Proteins)
RN  - 38183-12-9 (Fluorescamine)
SB  - IM
MH  - Alleles
MH  - Amino Acid Sequence
MH  - Autoimmunity
MH  - Fluorescamine/chemistry
MH  - Fluorescent Dyes/chemistry
MH  - Gene Expression
MH  - HLA-B27 Antigen/*chemistry/genetics/immunology
MH  - Humans
MH  - *Models, Immunological
MH  - Models, Molecular
MH  - Peptides/*chemistry/genetics/immunology
MH  - Protein Binding
MH  - Recombinant Proteins/chemistry/genetics/immunology
MH  - Staining and Labeling/methods
OTO - NOTNLM
OT  - Disease association
OT  - Fluorescence polarization
OT  - Gel filtration
OT  - Human leukocyte antigen
OT  - Peptide binding
EDAT- 2016/11/04 06:00
MHDA- 2017/06/06 06:00
CRDT- 2016/09/08 06:00
PHST- 2016/11/04 06:00 [pubmed]
PHST- 2017/06/06 06:00 [medline]
PHST- 2016/09/08 06:00 [entrez]
AID - 10.1007/s10930-016-9678-6 [pii]
AID - 10.1007/s10930-016-9678-6 [doi]
PST - ppublish
SO  - Protein J. 2016 Oct;35(5):346-353. doi: 10.1007/s10930-016-9678-6.