PMID- 27601296
OWN - NLM
STAT- MEDLINE
DCOM- 20170307
LR  - 20170922
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 57
DP  - 2016 Oct
TI  - Transcriptomic analysis of the head kidney of Topmouth culter (Culter alburnus) 
      infected with Flavobacterium columnare with an emphasis on phagosome pathway.
PG  - 413-418
LID - S1050-4648(16)30546-0 [pii]
LID - 10.1016/j.fsi.2016.09.001 [doi]
AB  - Flavobacterium columnare (FC) has caused worldwide fish columnaris disease with 
      high mortality and great economic losses in cultured fish, including Topmouth 
      culter (Culter alburnus). However, the knowledge about the host factors involved 
      in FC infection is little known. In this study, the transcriptomic profiles of 
      the head kidney from Topmouth culter with or without FC infection were obtained 
      using HiSeq 2500 (Illumina). Totally 79,641 unigenes with high quality were 
      obtained. Among them, 4037 differently expressed genes, including 1217 
      up-regulated and 2820 down-regulated genes, were identified and enriched using 
      databases of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes 
      (KEGG). The differently expressed genes were mainly associated with pathways such 
      as immune response, carbohydrate metabolism, amino acid metabolism, and lipid 
      metabolism. Since phagocytosis is a central mechanism of innate immune response 
      by host cells to defense against infectious agents, genes related to the 
      phagosome pathway were scrutinized and 9 differently expressed phagosome-related 
      genes were identified including 3 up-regulated and 6 down-regulated genes. Five 
      of them were further validated by quantitative real-time polymerase chain 
      reaction (qRT-PCR). This transcriptomic analysis of host genes in response to FC 
      infection provides data towards understanding the infection mechanisms and will 
      shed a new light on the prevention of columnaris.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Zhao, Lijuan
AU  - Zhao L
AD  - Shandong Freshwater Fisheries Research Institute, Shandong Provincial Key 
      Laboratory of Freshwater Genetics and Breeding, Jinan, Shandong, 250013, China; 
      Department of Aquatic Animal Medicine, College of Fisheries, Key Lab of 
      Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural 
      University, Wuhan, Hubei, 430070, China.
FAU - Tu, Jiagang
AU  - Tu J
AD  - Department of Aquatic Animal Medicine, College of Fisheries, Key Lab of 
      Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural 
      University, Wuhan, Hubei, 430070, China.
FAU - Zhang, Yulei
AU  - Zhang Y
AD  - Department of Aquatic Animal Medicine, College of Fisheries, Key Lab of 
      Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural 
      University, Wuhan, Hubei, 430070, China.
FAU - Wang, Jinfu
AU  - Wang J
AD  - Shandong Freshwater Fisheries Research Institute, Shandong Provincial Key 
      Laboratory of Freshwater Genetics and Breeding, Jinan, Shandong, 250013, China.
FAU - Yang, Ling
AU  - Yang L
AD  - Shandong Freshwater Fisheries Research Institute, Shandong Provincial Key 
      Laboratory of Freshwater Genetics and Breeding, Jinan, Shandong, 250013, China.
FAU - Wang, Weimin
AU  - Wang W
AD  - Department of Aquatic Animal Medicine, College of Fisheries, Key Lab of 
      Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural 
      University, Wuhan, Hubei, 430070, China.
FAU - Wu, Zaohe
AU  - Wu Z
AD  - College of Animal Sciences and Technology, Zhongkai University of Agriculture and 
      Engineering, Guangzhou, Guangdong, 510225, China.
FAU - Meng, Qinglei
AU  - Meng Q
AD  - Shandong Freshwater Fisheries Research Institute, Shandong Provincial Key 
      Laboratory of Freshwater Genetics and Breeding, Jinan, Shandong, 250013, China. 
      Electronic address: qingleimeng@126.com.
FAU - Lin, Li
AU  - Lin L
AD  - Department of Aquatic Animal Medicine, College of Fisheries, Key Lab of 
      Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural 
      University, Wuhan, Hubei, 430070, China; College of Animal Sciences and 
      Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 
      Guangdong, 510225, China. Electronic address: linli@mail.hzau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20160904
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Fish Proteins)
SB  - IM
MH  - Animals
MH  - *Cyprinidae
MH  - Fish Diseases/*genetics/immunology/microbiology
MH  - Fish Proteins/*genetics/metabolism
MH  - Flavobacteriaceae
MH  - Flavobacteriaceae Infections/genetics/immunology/microbiology/*veterinary
MH  - Flavobacterium/*physiology
MH  - Head Kidney/immunology
MH  - *Immunity, Innate
MH  - Phagosomes/immunology
MH  - *Transcriptome
OTO - NOTNLM
OT  - Culter alburnus
OT  - Differently expressed genes
OT  - Flavobacterium columnare
OT  - Phagosome
OT  - Transcriptome
EDAT- 2016/09/08 06:00
MHDA- 2017/03/08 06:00
CRDT- 2016/09/08 06:00
PHST- 2016/06/24 00:00 [received]
PHST- 2016/08/03 00:00 [revised]
PHST- 2016/09/02 00:00 [accepted]
PHST- 2016/09/08 06:00 [entrez]
PHST- 2016/09/08 06:00 [pubmed]
PHST- 2017/03/08 06:00 [medline]
AID - S1050-4648(16)30546-0 [pii]
AID - 10.1016/j.fsi.2016.09.001 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2016 Oct;57:413-418. doi: 10.1016/j.fsi.2016.09.001. Epub 
      2016 Sep 4.