PMID- 2760208 OWN - NLM STAT- MEDLINE DCOM- 19890921 LR - 20220311 IS - 0021-9738 (Print) IS - 0021-9738 (Linking) VI - 84 IP - 3 DP - 1989 Sep TI - Localization of transforming growth factor alpha and its receptor in gastric mucosal cells. Implications for a regulatory role in acid secretion and mucosal renewal. PG - 1017-23 AB - Transforming growth factor alpha (TGF alpha) shares with epidermal growth factor (EGF) structural homology (35%), a common cell-surface membrane receptor (TGF alpha/EGF receptor), and a nearly identical spectrum of biological activity, including inhibition of gastric acid secretion. Herein, we report expression of TGF alpha mRNA in normal gastric mucosa of the adult guinea pig, rat, and dog. TGF alpha mRNA was also detected in matched surgically resected gastric mucosa and adjacent gastric carcinoma from 10 patients, and in gastric mucosa adjacent to a benign ulcer from an additional patient. TGF alpha protein was quantitated by radioimmunoassay and was present in tumor and adjacent mucosa. TGF alpha/EGF receptor mRNA was also detected in gastric mucosa from all species studied. Localization of TGF alpha and TGF alpha/EGF receptor mRNA expression was examined in samples of unfractionated guinea pig gastric mucosa and from chief cell-enriched and parietal cell-enriched fractions. All samples exhibited TGF alpha and TGF alpha/EGF receptor expression. The TGF alpha signal was greatest in the parietal cell fraction (5.8-fold increase), but was also enhanced in the chief cell fraction (1.9-fold increase) relative to the unfractionated gastric mucosa. Like TGF alpha expression, TGF alpha/EGF receptor mRNA expression was most intense in the parietal cell-enriched fraction (7.8-fold increase), but was also increased in the chief cell-enriched fraction (2.7-fold increase) relative to the unfractionated guinea pig gastric mucosa. We conclude that TGF alpha and TGF alpha/EGF receptor genes are expressed in normal adult mammalian gastric mucosa. These findings, when interpreted in light of described actions of TGF alpha and EGF, provide evidence that local production of TGF alpha could play an important role in the regulation of acid secretion and mucosal renewal in the stomach. FAU - Beauchamp, R D AU - Beauchamp RD AD - Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232. FAU - Barnard, J A AU - Barnard JA FAU - McCutchen, C M AU - McCutchen CM FAU - Cherner, J A AU - Cherner JA FAU - Coffey, R J Jr AU - Coffey RJ Jr LA - eng GR - CA 46413/CA/NCI NIH HHS/United States GR - CA-01309/CA/NCI NIH HHS/United States PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Research Support, U.S. Gov't, Non-P.H.S. PT - Research Support, U.S. Gov't, P.H.S. PL - United States TA - J Clin Invest JT - The Journal of clinical investigation JID - 7802877 RN - 0 (DNA Probes) RN - 0 (RNA, Messenger) RN - 76057-06-2 (Transforming Growth Factors) RN - EC 2.7.10.1 (ErbB Receptors) SB - IM MH - Adult MH - Aged MH - Aged, 80 and over MH - Animals MH - Blotting, Northern MH - Carcinoma/analysis/metabolism MH - DNA Probes MH - Dogs MH - ErbB Receptors/*isolation & purification/physiology MH - Gastric Acid/*metabolism MH - Gastric Mucosa/*metabolism/physiology MH - Guinea Pigs MH - Humans MH - Middle Aged MH - RNA, Messenger/isolation & purification MH - Rats MH - Stomach Neoplasms/analysis/metabolism MH - Transforming Growth Factors/*isolation & purification/metabolism/physiology PMC - PMC329750 EDAT- 1989/09/01 00:00 MHDA- 2001/03/28 10:01 PMCR- 1989/09/01 CRDT- 1989/09/01 00:00 PHST- 1989/09/01 00:00 [pubmed] PHST- 2001/03/28 10:01 [medline] PHST- 1989/09/01 00:00 [entrez] PHST- 1989/09/01 00:00 [pmc-release] AID - 10.1172/JCI114223 [doi] PST - ppublish SO - J Clin Invest. 1989 Sep;84(3):1017-23. doi: 10.1172/JCI114223.