PMID- 27603546
OWN - NLM
STAT- MEDLINE
DCOM- 20170508
LR  - 20181202
IS  - 1750-3841 (Electronic)
IS  - 0022-1147 (Linking)
VI  - 81
IP  - 10
DP  - 2016 Oct
TI  - Identification of the Critical Amino Acid Residues of Immunoglobulin E and 
      Immunoglobulin G Epitopes in alpha-Lactalbumin by Alanine Scanning Analysis.
PG  - T2597-T2603
LID - 10.1111/1750-3841.13425 [doi]
AB  - alpha-Lactalbumin represents one of the major allergens causing cow milk allergy. Few 
      studies have clearly evaluated immunological relationships between immunoglobulin 
      E (IgE) and immunoglobulin G (IgG)-binding epitopes of alpha-lactalbumin. IgE- and 
      IgG-binding epitopes were immunolabeled with individual sera from cow 
      milk-allergic patients. Alanine scanning of immunodominant epitopes was used to 
      identify the critical amino acid (aa). Our initial data revealed Val(8) , Phe(9) 
      , Arg(10) , Tyr(103) , Leu(105) , and His(107) were the critical aa for 
      IgE-binding epitope. The critical aa of IgG-binding epitopes were Phe(9) , 
      Leu(15) , Pro(24) , Trp(26) , and His(32) . This study will provide necessary 
      information to alter the cDNA to encode a protein capable of activating 
      milk-specific T cells, but with reduced IgE- or IgG-binding capacity.
CI  - (c) 2016 Institute of Food Technologists(R).
FAU - Yanjun, Cong
AU  - Yanjun C
AD  - Beijing Key Laboratory of Food Flavor Chemistry & Beijing Higher Inst. 
      Engineering Research Center of Food Additives and Ingredients, Beijing Technology 
      and Business Univ, Haidian District, Beijing, 100048, P. R. China. 
      congyj@th.btbu.edu.cn.
FAU - Shengyun, Zhou
AU  - Shengyun Z
AD  - Beijing Key Laboratory of Food Flavor Chemistry & Beijing Higher Inst. 
      Engineering Research Center of Food Additives and Ingredients, Beijing Technology 
      and Business Univ, Haidian District, Beijing, 100048, P. R. China.
FAU - Linfeng, Li
AU  - Linfeng L
AD  - Dept. of Dermatology, Beijing Friendship Hospital, Capital Medical Univ, Xichen 
      District, Beijing, 100050, P. R. China.
LA  - eng
PT  - Journal Article
DEP - 20160907
PL  - United States
TA  - J Food Sci
JT  - Journal of food science
JID - 0014052
RN  - 0 (Allergens)
RN  - 0 (Caseins)
RN  - 0 (DNA, Complementary)
RN  - 0 (Epitopes)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Peptides)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9013-90-5 (Lactalbumin)
RN  - OF5P57N2ZX (Alanine)
SB  - IM
MH  - Alanine/*chemistry
MH  - Allergens/immunology
MH  - Amino Acid Sequence
MH  - Animals
MH  - Caseins/chemistry
MH  - Cattle
MH  - Child, Preschool
MH  - DNA, Complementary/metabolism
MH  - Epitopes/*immunology
MH  - Humans
MH  - Immunoglobulin E/*immunology
MH  - Immunoglobulin G/*immunology
MH  - Infant
MH  - Lactalbumin/*chemistry
MH  - Milk/immunology
MH  - Milk Hypersensitivity/*immunology
MH  - Peptides/chemistry
MH  - Protein Binding
MH  - Protein Conformation
MH  - Signal Transduction
OTO - NOTNLM
OT  - cow milk allergy
OT  - critical amino acid
OT  - epitope
OT  - alpha-lactalbumin
EDAT- 2016/09/08 06:00
MHDA- 2017/05/10 06:00
CRDT- 2016/09/08 06:00
PHST- 2015/11/18 00:00 [received]
PHST- 2016/06/06 00:00 [revised]
PHST- 2016/07/20 00:00 [accepted]
PHST- 2016/09/08 06:00 [pubmed]
PHST- 2017/05/10 06:00 [medline]
PHST- 2016/09/08 06:00 [entrez]
AID - 10.1111/1750-3841.13425 [doi]
PST - ppublish
SO  - J Food Sci. 2016 Oct;81(10):T2597-T2603. doi: 10.1111/1750-3841.13425. Epub 2016 
      Sep 7.