PMID- 27604750
OWN - NLM
STAT- MEDLINE
DCOM- 20170302
LR  - 20181202
IS  - 1749-0774 (Electronic)
IS  - 0914-7470 (Linking)
VI  - 30
IP  - 1
DP  - 2017 Jan
TI  - Glycogen synthase kinase 3 (GSK3)-inhibitor SB216763 promotes the conversion of 
      human umbilical cord mesenchymal stem cells into neural precursors in adherent 
      culture.
PG  - 11-22
LID - 10.1007/s13577-016-0146-6 [doi]
AB  - The ability to generate neural progenitor cells from human umbilical cord 
      mesenchymal stem cells (hUC-MSCs) has provided an option to treat 
      neurodegenerative diseases. To establish a method for this purpose, we 
      characterized the early neural markers of hUC-MSCs-derived cells under different 
      conditions. We found that neither the elimination of signals for alternative fate 
      nor N2 supplement was sufficient to differentiate hUC-MSCs into neural precursor 
      cells, but the GSK3 inhibitor SB216763 could promote an efficient neural 
      commitment of hUC-MSCs. The results indicated that Wnt/beta-catenin might play an 
      important role during the early neural differentiation of hUC-MSCs. Here, we 
      report a method for hUC-MSCs to commit efficiently into a neural fate within a 
      short period of time. This protocol provides an efficient method for 
      hUC-MSCs-based neural regeneration.
FAU - Gao, Liyang
AU  - Gao L
AD  - Stem Cell Research Center, Affiliated Hospital of Guangdong Medical University, 
      Zhanjiang, China. pandarun@aliyun.com.
FAU - Zhao, Mingyan
AU  - Zhao M
AD  - Stem Cell Research Center, Affiliated Hospital of Guangdong Medical University, 
      Zhanjiang, China.
FAU - Li, Peng
AU  - Li P
AD  - Stem Cell Research Center, Affiliated Hospital of Guangdong Medical University, 
      Zhanjiang, China.
FAU - Kong, Junchao
AU  - Kong J
AD  - Department of Spinal Surgery, Affiliated Hospital of Guangdong Medical 
      University, Zhanjiang, China.
FAU - Liu, Zhijun
AU  - Liu Z
AD  - Department of Spinal Surgery, Affiliated Hospital of Guangdong Medical 
      University, Zhanjiang, China.
FAU - Chen, Yonghua
AU  - Chen Y
AD  - Department of Pathology, Affiliated Hospital of Guangdong Medical University, 
      Zhanjiang, China.
FAU - Huang, Rui
AU  - Huang R
AD  - Stem Cell Research Center, Affiliated Hospital of Guangdong Medical University, 
      Zhanjiang, China.
FAU - Chu, Jiaqi
AU  - Chu J
AD  - Stem Cell Research Center, Affiliated Hospital of Guangdong Medical University, 
      Zhanjiang, China.
FAU - Quan, Juanhua
AU  - Quan J
AD  - Department of Gastroenterology, Affiliated Hospital of Guangdong Medical 
      University, Zhanjiang, China.
FAU - Zeng, Rong
AU  - Zeng R
AD  - Department of Spinal Surgery, Affiliated Hospital of Guangdong Medical 
      University, Zhanjiang, China. 44147103@qq.com.
LA  - eng
PT  - Journal Article
DEP - 20160907
PL  - Japan
TA  - Hum Cell
JT  - Human cell
JID - 8912329
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Indoles)
RN  - 0 (Maleimides)
RN  - 0 (SB 216763)
RN  - 0 (Wnt Proteins)
RN  - 0 (beta Catenin)
RN  - EC 2.7.11.26 (Glycogen Synthase Kinase 3)
SB  - IM
EIN - Hum Cell. 2017 Jan;30(1):49. PMID: 27826801
MH  - Cell Differentiation/*drug effects/*genetics
MH  - Cells, Cultured
MH  - Enzyme Inhibitors/*pharmacology
MH  - Glycogen Synthase Kinase 3/*antagonists & inhibitors
MH  - Humans
MH  - Indoles/*pharmacology
MH  - Maleimides/*pharmacology
MH  - Mesenchymal Stem Cells/*cytology
MH  - Nerve Regeneration/drug effects/genetics
MH  - Neurons/*cytology
MH  - Regenerative Medicine/methods
MH  - Signal Transduction/genetics/physiology
MH  - Stem Cells/*cytology
MH  - Umbilical Cord/*cytology
MH  - Wnt Proteins/physiology
MH  - beta Catenin/physiology
OTO - NOTNLM
OT  - Default mechanism
OT  - GSK3 inhibitor
OT  - Human umbilical cord mesenchymal stem cells
OT  - N2 supplement
OT  - Neural differentiation
OT  - Neural precursor cells
EDAT- 2016/09/09 06:00
MHDA- 2017/03/03 06:00
CRDT- 2016/09/09 06:00
PHST- 2016/07/04 00:00 [received]
PHST- 2016/08/26 00:00 [accepted]
PHST- 2016/09/09 06:00 [pubmed]
PHST- 2017/03/03 06:00 [medline]
PHST- 2016/09/09 06:00 [entrez]
AID - 10.1007/s13577-016-0146-6 [pii]
AID - 10.1007/s13577-016-0146-6 [doi]
PST - ppublish
SO  - Hum Cell. 2017 Jan;30(1):11-22. doi: 10.1007/s13577-016-0146-6. Epub 2016 Sep 7.