PMID- 27608441
OWN - NLM
STAT- MEDLINE
DCOM- 20171030
LR  - 20180315
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 118
IP  - 3
DP  - 2017 Mar
TI  - Expression of Six Proteins Causes Reprogramming of Porcine Fibroblasts Into 
      Induced Pluripotent Stem Cells With Both Active X Chromosomes.
PG  - 537-553
LID - 10.1002/jcb.25727 [doi]
AB  - In this study, we created porcine-induced pluripotent stem (iPS) cells with the 
      expression of six reprogramming factors (Oct3/4, Klf4, Sox2, c-Myc, Lin28, and 
      Nanog). The resulting cells showed growth dependent on LIF (leukemia inhibitory 
      factor) and expression of multiple stem cell markers. Furthermore, the iPS cells 
      caused teratoma formation with three layers of differentiation and had both 
      active X chromosomes (XaXa). Our iPS cells satisfied the both of important 
      characteristics of stem cells: teratoma formation and activation of both X 
      chromosomes. Injection of these iPS cells into morula stage embryos showed that 
      these cells participate in the early stage of porcine embryogenesis. Furthermore, 
      the RNA-Seq analysis detected that expression levels of endogenous pluripotent 
      related genes, NANOG, SOX2, ZFP42, OCT3/4, ESRRB, and ERAS were much higher in 
      iPS with six factors than that with four reprogramming factors. We can conclude 
      that the expression of six reprogramming factors enables the creation of porcine 
      iPS cells, which is partially close to naive iPS state. J. Cell. Biochem. 118: 
      537-553, 2017. (c) 2016 Wiley Periodicals, Inc.
CI  - (c) 2016 Wiley Periodicals, Inc.
FAU - Fukuda, Tomokazu
AU  - Fukuda T
AD  - United Graduate School of Agricultural Sciences, Iwate University, 4-3-5, Ueda, 
      Morioka 020-8551, Iwate, Japan.
FAU - Tani, Tetsuya
AU  - Tani T
AD  - Laboratory of Animal Reproduction, Department of Advanced Bioscience, Faculty of 
      Agriculture, Kindai University, 3327-204 Nakamachi, Nara 631-8505, Japan.
FAU - Haraguchi, Seiki
AU  - Haraguchi S
AD  - Division of Animal Sciences, Animal Biotechnology Unit, Institute of 
      Agrobiological Sciences, National Agriculture and Food Research Organization 
      (NARO), Tsukuba, Ibaraki 305-0901, Japan.
FAU - Donai, Kenichiro
AU  - Donai K
AD  - Graduate School of Agricultural Science, Tohoku University, Sendai, 981-8555, 
      Japan.
FAU - Nakajima, Nobuyoshi
AU  - Nakajima N
AD  - Center for Environmental Biology and Ecosystem Studies, National Institute of 
      Environmental Studies, Tsukuba, Japan.
FAU - Uenishi, Hirohide
AU  - Uenishi H
AD  - Animal Bioregulation Unit, Division of Animal Sciences, Institute of 
      Agrobiological Sciences, National Agriculture and Food Research Organization 
      (NARO), 1-2 Owashi, Tsukuba, Ibaraki 305-8634, Japan.
FAU - Eitsuka, Takahiro
AU  - Eitsuka T
AD  - Faculty of Applied Life Sciences, Niigata University of Pharmacy and Applied Life 
      Sciences, Niigata, Japan.
FAU - Miyagawa, Makoto
AU  - Miyagawa M
AD  - Central Experimental Animal Center, Teikyo University School of Medicine, Japan.
FAU - Song, Sanghoun
AU  - Song S
AD  - Faculty of Life and Environmental Science, Shimane University, Matsue, Shimane, 
      Japan.
FAU - Onuma, Manabu
AU  - Onuma M
AD  - Center for Environmental Biology and Ecosystem Studies, National Institute of 
      Environmental Studies, Tsukuba, Japan.
FAU - Hoshino, Yumi
AU  - Hoshino Y
AD  - Laboratory of Reproductive Endocrinology, Graduate School of Biosphere Science, 
      Hiroshima University, Higashi-Hiroshima, Kagamiyama 1-4-4, Hiroshima 739-8528, 
      Japan.
FAU - Sato, Eimei
AU  - Sato E
AD  - National Livestock Breeding Center, Odakurahara, Odakura, Nishigo-mura, 
      Nishishirakawa-gun, Fukushima 961-8511, Japan.
FAU - Honda, Arata
AU  - Honda A
AD  - Organization for Promotion of Tenure Track, University of Miyazaki, 5200 Kihara, 
      Kiyotake, Miyazaki, 889-1692, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20161017
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - *Cellular Reprogramming Techniques
MH  - Female
MH  - *Gene Expression
MH  - Induced Pluripotent Stem Cells/*metabolism
MH  - Swine
MH  - Transcription Factors/*biosynthesis
MH  - X Chromosome/*metabolism
OTO - NOTNLM
OT  - EPIGENETIC STATUS
OT  - PLURIPOTENT STEM CELL
OT  - PORCINE
OT  - TRANSCRIPTIONAL FACTORS
OT  - X CHROMOSOME
EDAT- 2016/10/28 06:00
MHDA- 2017/10/31 06:00
CRDT- 2016/09/09 06:00
PHST- 2016/02/07 00:00 [received]
PHST- 2016/09/06 00:00 [accepted]
PHST- 2016/10/28 06:00 [pubmed]
PHST- 2017/10/31 06:00 [medline]
PHST- 2016/09/09 06:00 [entrez]
AID - 10.1002/jcb.25727 [doi]
PST - ppublish
SO  - J Cell Biochem. 2017 Mar;118(3):537-553. doi: 10.1002/jcb.25727. Epub 2016 Oct 
      17.