PMID- 27609570
OWN - NLM
STAT- MEDLINE
DCOM- 20170627
LR  - 20170627
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 32
IP  - 9
DP  - 2016 Sep
TI  - [Macrophages promote the migration of neural stem cells into mouse spinal cord 
      injury site].
PG  - 1174-7
AB  - Objective To explore the role of macrophages in the migration of neural stem 
      cells (NSCs) in vivo and in vitro . Methods NSCs with green fluorescent protein 
      (GFP) were isolated from GFP transgenic mice and the immunofluorescence 
      cytochemical staining of nestin was used to identify NSCs. After spinal cord 
      injury was induced, the tissue level of macrophage chemotactic protein-1 (MCP-1) 
      mRNA was detected using quantitative real time PCR. The migration of GFP-NSCs was 
      investigated 1 week after GFP-NSCs were injected into both sides of the damaged 
      area. The effect of macrophage on the migration of NSCs in vitro was tested by 
      Transwell(TM) system and the content of MCP-1 was detected by ELISA. Results NSCs 
      highly expressed nestin. Compared with the control group, the level of MCP-1 mRNA 
      significantly increased in the spinal cord injury group. The NSCs which were 
      injected into the spinal cord migrated into the center of the injured site where 
      F4/80 was highly expressed. Macrophages significantly increased the number of 
      migrating NSCs in vitro and the secretion of MCP-1. Conclusion Macrophages induce 
      NSC migrating into the spinal cord injury site possibly through promoting the 
      secretion of MCP-1.
FAU - Cheng, Zhijian
AU  - Cheng Z
AD  - Department of Orthopaedics, Second Affiliated Hospital, Xi'an Jiaotong 
      University, Xi'an 710004, China.
FAU - Zhu, Wen
AU  - Zhu W
AD  - Deparment of Critical Care Medicine, First People's Hospital of Xianyany City, 
      Xianyang 710021, China.
FAU - Li, Haopeng
AU  - Li H
AD  - Department of Orthopaedics, Second Affiliated Hospital, Xi'an Jiaotong 
      University, Xi'an 710004, China.
FAU - He, Xijing
AU  - He X
AD  - Department of Orthopaedics, Second Affiliated Hospital, Xi'an Jiaotong 
      University, Xi'an 710004, China. *Corresponding author, E-mail: 
      xijing_h@vip.tom.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Animals
MH  - *Cell Movement
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Female
MH  - Humans
MH  - Macrophages/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neurons/*cytology
MH  - Spinal Cord Injuries/genetics/metabolism/*physiopathology
MH  - Stem Cells/*cytology
EDAT- 2016/09/10 06:00
MHDA- 2017/06/28 06:00
CRDT- 2016/09/10 06:00
PHST- 2016/09/10 06:00 [entrez]
PHST- 2016/09/10 06:00 [pubmed]
PHST- 2017/06/28 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2016 Sep;32(9):1174-7.