PMID- 27610141
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160909
LR  - 20220310
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2016
DP  - 2016
TI  - Comparison of Osteogenesis between Adipose-Derived Mesenchymal Stem Cells and 
      Their Sheets on Poly-epsilon-Caprolactone/beta-Tricalcium Phosphate Composite Scaffolds in 
      Canine Bone Defects.
PG  - 8414715
LID - 10.1155/2016/8414715 [doi]
LID - 8414715
AB  - Multipotent mesenchymal stem cells (MSCs) and MSC sheets have effective 
      potentials of bone regeneration. Composite polymer/ceramic scaffolds such as 
      poly-epsilon-caprolactone (PCL)/beta-tricalcium phosphate (beta-TCP) are widely used to 
      repair large bone defects. The present study investigated the in vitro osteogenic 
      potential of canine adipose-derived MSCs (Ad-MSCs) and Ad-MSC sheets. Composite 
      PCL/beta-TCP scaffolds seeded with Ad-MSCs or wrapped with osteogenic Ad-MSC sheets 
      (OCS) were also fabricated and their osteogenic potential was assessed following 
      transplantation into critical-sized bone defects in dogs. The alkaline 
      phosphatase (ALP) activity of osteogenic Ad-MSCs (O-MSCs) and OCS was 
      significantly higher than that of undifferentiated Ad-MSCs (U-MSCs). The ALP, 
      runt-related transcription factor 2, osteopontin, and bone morphogenetic protein 
      7 mRNA levels were upregulated in O-MSCs and OCS as compared to U-MSCs. In a 
      segmental bone defect, the amount of newly formed bone was greater in 
      PCL/beta-TCP/OCS and PCL/beta-TCP/O-MSCs/OCS than in the other groups. The OCS exhibit 
      strong osteogenic capacity, and OCS combined with a PCL/beta-TCP composite scaffold 
      stimulated new bone formation in a critical-sized bone defect. These results 
      suggest that the PCL/beta-TCP/OCS composite has potential clinical applications in 
      bone regeneration and can be used as an alternative treatment modality in bone 
      tissue engineering.
FAU - Kim, Yongsun
AU  - Kim Y
AD  - BK21 PLUS Program for Creative Veterinary Science Research, Research Institute 
      for Veterinary Science and College of Veterinary Medicine, Seoul National 
      University, Seoul 08826, Republic of Korea.
FAU - Lee, Seung Hoon
AU  - Lee SH
AD  - BK21 PLUS Program for Creative Veterinary Science Research, Research Institute 
      for Veterinary Science and College of Veterinary Medicine, Seoul National 
      University, Seoul 08826, Republic of Korea.
FAU - Kang, Byung-Jae
AU  - Kang BJ
AD  - College of Veterinary Medicine and Institute of Veterinary Science, Kangwon 
      National University, Chuncheon 24341, Republic of Korea.
FAU - Kim, Wan Hee
AU  - Kim WH
AD  - BK21 PLUS Program for Creative Veterinary Science Research, Research Institute 
      for Veterinary Science and College of Veterinary Medicine, Seoul National 
      University, Seoul 08826, Republic of Korea.
FAU - Yun, Hui-Suk
AU  - Yun HS
AD  - Powder & Ceramics Division, Korea Institute of Materials Science, Changwon 51508, 
      Republic of Korea.
FAU - Kweon, Oh-Kyeong
AU  - Kweon OK
AUID- ORCID: 0000-0003-2670-7322
AD  - BK21 PLUS Program for Creative Veterinary Science Research, Research Institute 
      for Veterinary Science and College of Veterinary Medicine, Seoul National 
      University, Seoul 08826, Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20160816
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC5004032
EDAT- 2016/09/10 06:00
MHDA- 2016/09/10 06:01
PMCR- 2016/08/16
CRDT- 2016/09/10 06:00
PHST- 2016/05/16 00:00 [received]
PHST- 2016/07/01 00:00 [revised]
PHST- 2016/07/05 00:00 [accepted]
PHST- 2016/09/10 06:00 [entrez]
PHST- 2016/09/10 06:00 [pubmed]
PHST- 2016/09/10 06:01 [medline]
PHST- 2016/08/16 00:00 [pmc-release]
AID - 10.1155/2016/8414715 [doi]
PST - ppublish
SO  - Stem Cells Int. 2016;2016:8414715. doi: 10.1155/2016/8414715. Epub 2016 Aug 16.