PMID- 27613664 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20191120 IS - 1474-9726 (Electronic) IS - 1474-9718 (Print) IS - 1474-9718 (Linking) VI - 15 IP - 6 DP - 2016 Dec TI - Exercise reverses age-related vulnerability of the retina to injury by preventing complement-mediated synapse elimination via a BDNF-dependent pathway. PG - 1082-1091 LID - 10.1111/acel.12512 [doi] AB - Retinal ganglion cells (RGCs) become increasingly vulnerable to injury with advancing age. We recently showed that this vulnerability can be strongly modified in mice by exercise. However, the characteristics and underlying mechanisms of retinal protection with exercise remain unknown. Hence, the aim of this study was to investigate cellular changes associated with exercise-induced protection of aging retinal cells and the role of local and peripheral trophic signalling in mediating these effects. We focussed on two molecules that are thought to play key roles in mediating beneficial effects of exercise: brain-derived neurotrophic factor (BDNF) and AMP-activated protein kinase (AMPK). In middle-aged (12 months old) C57BL/6J mice, we found that exercise protected RGCs against dysfunction and cell loss after an acute injury induced by elevation of intra-ocular pressure. This was associated with preservation of inner retinal synapses and reduced synaptic complement deposition. Retinal expression of BDNF was not upregulated in response to exercise alone. Rather, exercise maintained BDNF levels in the retina, which were decreased postinjury in nonexercised animals. Confirming a critical role for BDNF, we found that blocking BDNF signalling during exercise by pharmacological means or genetic knock-down suppressed the functional protection of RGCs afforded by exercise. Protection of RGCs with exercise was independent of activation of AMPK in either retina or skeletal muscle. Our data support a previously unidentified mechanism in which exercise prevents loss of BDNF in the retina after injury and preserves neuronal function and survival by preventing complement-mediated elimination of synapses. CI - (c) 2016 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd. FAU - Chrysostomou, Vicki AU - Chrysostomou V AD - Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, University of Melbourne, 32 Gisborne Street, East Melbourne, Vic., 3002, Australia. FAU - Galic, Sandra AU - Galic S AD - St. Vincent's Institute of Medical Research and Department of Medicine, University of Melbourne, 41 Victoria Parade, Fitzroy, Vic., 3065, Australia. FAU - van Wijngaarden, Peter AU - van Wijngaarden P AD - Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, University of Melbourne, 32 Gisborne Street, East Melbourne, Vic., 3002, Australia. FAU - Trounce, Ian A AU - Trounce IA AD - Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, University of Melbourne, 32 Gisborne Street, East Melbourne, Vic., 3002, Australia. FAU - Steinberg, Gregory R AU - Steinberg GR AD - Division of Endocrinology and Metabolism, Department of Medicine, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada. AD - Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada. FAU - Crowston, Jonathan G AU - Crowston JG AD - Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, University of Melbourne, 32 Gisborne Street, East Melbourne, Vic., 3002, Australia. LA - eng GR - The Ophthalmic Research Institute of Australia/ GR - RANZCO Eye Foundation Grant/ GR - WA Quinlivan/Glaucoma Australia Grant/ GR - APP1068813/National Health and Medical Research Council/ GR - Victorian Government/ PT - Journal Article DEP - 20160909 PL - England TA - Aging Cell JT - Aging cell JID - 101130839 PMC - PMC5114604 OTO - NOTNLM OT - anti-aging OT - brain-derived neurotrophic factor OT - exercise OT - mouse model OT - neuroprotection OT - retinal ganglion cell EDAT- 2016/09/11 06:00 MHDA- 2016/09/11 06:01 PMCR- 2016/12/01 CRDT- 2016/09/11 06:00 PHST- 2016/07/12 00:00 [accepted] PHST- 2016/09/11 06:00 [pubmed] PHST- 2016/09/11 06:01 [medline] PHST- 2016/09/11 06:00 [entrez] PHST- 2016/12/01 00:00 [pmc-release] AID - ACEL12512 [pii] AID - 10.1111/acel.12512 [doi] PST - ppublish SO - Aging Cell. 2016 Dec;15(6):1082-1091. doi: 10.1111/acel.12512. Epub 2016 Sep 9.