PMID- 2761554
OWN - NLM
STAT- MEDLINE
DCOM- 19890913
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 213
IP  - 2
DP  - 1989 Aug
TI  - The effects of 1,6-dinitropyrene on spindle morphology in transformed human 
      cells.
PG  - 141-8
AB  - The effects of 1,6-dinitropyrene (1,6-DNP) on the fidelity of cell division were 
      studied in the transformed human fibroblast cell line MRC5VA. Over a dose range 
      of 0.1-10 micrograms/ml of 1.6-DNP, we observed significant increases in the 
      levels of abnormal division stages, associated with damage to the spindle 
      apparatus of the cell. Qualitative changes in spindle morphology and a 
      quantitative decrease in pole-to-pole spindle length were also observed with 
      increasing doses of 1.6-DNP. Such changes in the size and morphology of the 
      spindle corresponded with an accumulation of cells blocked at metaphase. The 
      presence of catalase did not modify the response, suggesting that the effects on 
      the spindle apparatus and cell division were not caused by the generation of 
      radicals but by the direct action of 1.6-DNP.
FAU - Adams, K
AU  - Adams K
AD  - Biomedical and Physiological Research Group, School of Biological Sciences, 
      University College of Swansea, Great Britain.
FAU - Lafi, A
AU  - Lafi A
FAU - Parry, J M
AU  - Parry JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Pyrenes)
RN  - 66Q2ZUF83N (1,6-dinitropyrene)
RN  - EC 1.11.1.6 (Catalase)
SB  - IM
MH  - Catalase/pharmacology
MH  - Cell Division/drug effects
MH  - Cell Line, Transformed
MH  - Humans
MH  - Mitotic Index
MH  - Pyrenes/*toxicity
MH  - Spindle Apparatus/*drug effects
EDAT- 1989/08/01 00:00
MHDA- 1989/08/01 00:01
CRDT- 1989/08/01 00:00
PHST- 1989/08/01 00:00 [pubmed]
PHST- 1989/08/01 00:01 [medline]
PHST- 1989/08/01 00:00 [entrez]
AID - 0027-5107(89)90145-0 [pii]
AID - 10.1016/0027-5107(89)90145-0 [doi]
PST - ppublish
SO  - Mutat Res. 1989 Aug;213(2):141-8. doi: 10.1016/0027-5107(89)90145-0.