PMID- 27616197
OWN - NLM
STAT- MEDLINE
DCOM- 20170731
LR  - 20191210
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 237
DP  - 2016 Nov
TI  - Development and evaluation of a new lateral flow assay for simultaneous detection 
      of antibodies against African Horse Sickness and Equine Infectious Anemia 
      viruses.
PG  - 127-131
LID - S0166-0934(16)30164-1 [pii]
LID - 10.1016/j.jviromet.2016.09.009 [doi]
AB  - African horse sickness (AHS) and equine infectious anemia (EIA) are both 
      notifiable equid specific diseases that may present similar clinical signs. 
      Considering the increased global movement of horses and equine products over the 
      past decades, together with the socio-economic impact of previous AHS and EIA 
      outbreaks, there is a clear demand for an early discrimination and a strict 
      control of their transmission between enzootic and AHS/EIA-free regions. 
      Currently, the individual control and prevention of AHS or EIA relies on a series 
      of measures, including the restriction of animal movements, vector control, and 
      the use of several laboratory techniques for viral identification, amongst 
      others. Despite being widely employed in surveillance programmes and in the 
      control of animal movements, the available serological assays can only detect 
      AHS- or EIA-specific antibodies individually. In this work, a duplex lateral flow 
      assay (LFA) for simultaneous detection and differentiation of specific antibodies 
      against AHS virus (AHSV) and EIA virus (EIAV) was developed and evaluated with 
      experimental and field serum samples. The duplex LFA was based on the AHSV-VP7 
      outer core protein and the EIAV-P26 major core protein. The results indicated 
      that the duplex LFA presented a good analytical performance, detecting 
      simultaneously and specifically antibodies against AHSV and EIAV. The initial 
      diagnostic evaluation revealed a good agreement with results from the AHS and EIA 
      tests prescribed by the OIE, and it highlighted the usefulness of the new 
      AHSV/EIAV duplex LFA for an on-field and point-of-care first diagnosis.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Costa, Sofia
AU  - Costa S
AD  - Inmunologia y Genetica Aplicada S.A. (INGENASA), Madrid, Spain.
FAU - Sastre, Patricia
AU  - Sastre P
AD  - Inmunologia y Genetica Aplicada S.A. (INGENASA), Madrid, Spain.
FAU - Perez, Teresa
AU  - Perez T
AD  - Inmunologia y Genetica Aplicada S.A. (INGENASA), Madrid, Spain.
FAU - Tapia, Istar
AU  - Tapia I
AD  - Inmunologia y Genetica Aplicada S.A. (INGENASA), Madrid, Spain.
FAU - Barrandeguy, Maria
AU  - Barrandeguy M
AD  - Instituto de Virologia, CICVyA, INTA, Buenos Aires, Argentina; Escuela de 
      Veterinaria, Universidad del Salvador, Buenos Aires, Argentina.
FAU - Sanchez-Vizcaino, Jose M
AU  - Sanchez-Vizcaino JM
AD  - VISAVET Health Surveillance Centre, Spain; Animal Health Department, UCM, Spain.
FAU - Sanchez-Matamoros, Almudena
AU  - Sanchez-Matamoros A
AD  - VISAVET Health Surveillance Centre, Spain; Animal Health Department, UCM, Spain.
FAU - Wigdorovitz, Andres
AU  - Wigdorovitz A
AD  - Instituto de Virologia, CICVyA, INTA, Buenos Aires, Argentina.
FAU - Sanz, Antonio
AU  - Sanz A
AD  - Inmunologia y Genetica Aplicada S.A. (INGENASA), Madrid, Spain.
FAU - Rueda, Paloma
AU  - Rueda P
AD  - Inmunologia y Genetica Aplicada S.A. (INGENASA), Madrid, Spain. Electronic 
      address: prueda@ingenasa.com.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20160909
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Antibodies, Viral)
RN  - 0 (Viral Core Proteins)
RN  - 0 (equine infectious anemia virus, p26)
SB  - IM
MH  - African Horse Sickness/*diagnosis/immunology
MH  - African Horse Sickness Virus/*immunology
MH  - Animals
MH  - Antibodies, Viral/*blood
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Equine Infectious Anemia/*diagnosis/immunology
MH  - Horses
MH  - Infectious Anemia Virus, Equine/*immunology
MH  - Point-of-Care Systems
MH  - Viral Core Proteins/immunology
OTO - NOTNLM
OT  - African horse sickness
OT  - Antibodies
OT  - Equine infectious anaemia
OT  - Lateral flow assay
EDAT- 2016/09/13 06:00
MHDA- 2017/08/02 06:00
CRDT- 2016/09/13 06:00
PHST- 2016/04/04 00:00 [received]
PHST- 2016/09/07 00:00 [revised]
PHST- 2016/09/07 00:00 [accepted]
PHST- 2016/09/13 06:00 [pubmed]
PHST- 2017/08/02 06:00 [medline]
PHST- 2016/09/13 06:00 [entrez]
AID - S0166-0934(16)30164-1 [pii]
AID - 10.1016/j.jviromet.2016.09.009 [doi]
PST - ppublish
SO  - J Virol Methods. 2016 Nov;237:127-131. doi: 10.1016/j.jviromet.2016.09.009. Epub 
      2016 Sep 9.