PMID- 27616282
OWN - NLM
STAT- MEDLINE
DCOM- 20170308
LR  - 20170308
IS  - 1618-0607 (Electronic)
IS  - 1438-4221 (Linking)
VI  - 306
IP  - 8
DP  - 2016 Dec
TI  - Staphylococcus aureus PSM peptides induce tolerogenic dendritic cells upon 
      treatment with ligands of extracellular and intracellular TLRs.
PG  - 666-674
LID - S1438-4221(16)30242-9 [pii]
LID - 10.1016/j.ijmm.2016.09.002 [doi]
AB  - Dendritic cells (DCs) are key players of the immune system and thus a target for 
      immune evasion by pathogens. We recently showed that the virulence factor 
      phenol-soluble modulin (PSM) produced by community-associated 
      methicillin-resistant Staphylococcus aureus strains induces tolerogenic DCs upon 
      Toll-like receptor (TLR) 2 activation via the p38-CREB-IL-10 pathway. Here, we 
      addressed the question whether this tolerogenic phenotype of DCs induced by PSMs 
      is specific for TLR2 activation. Therefore, bone marrow-derived DCs were treated 
      with various ligands for extracellular and intracellular TLRs simultaneously with 
      PSMalpha3. We show that PSMalpha3 modulates antigen uptake, maturation and cytokine 
      production of DCs activated by TLR1/2, TLR2/6, TLR4, TLR7, and TLR9. 
      Pre-incubation of DCs with a p38 MAP kinase inhibitor prevented the PSMalpha3-induced 
      IL-10 secretion, as well as MHC class II up-regulation upon TLR activation. In 
      consequence, the tolerogenic DCs induced by PSMalpha3 in response to several TLR 
      ligands promoted priming of regulatory T cells. Thus, PSMs could be useful as 
      inducers of tolerogenic DCs upon TLR ligand stimulation for therapeutic 
      applications.
CI  - Copyright A(c) 2016 Elsevier GmbH. All rights reserved.
FAU - Armbruster, Nicole S
AU  - Armbruster NS
AD  - Department of Internal Medicine II, University of Tubingen, Tubingen, Germany.
FAU - Richardson, Jennifer R
AU  - Richardson JR
AD  - Department of Internal Medicine II, University of Tubingen, Tubingen, Germany.
FAU - Schreiner, Jens
AU  - Schreiner J
AD  - Institute for Cell Biology, University of Tubingen, Tubingen, Germany.
FAU - Klenk, Juliane
AU  - Klenk J
AD  - Department of Internal Medicine II, University of Tubingen, Tubingen, Germany.
FAU - Gunter, Manina
AU  - Gunter M
AD  - Department of Internal Medicine II, University of Tubingen, Tubingen, Germany.
FAU - Autenrieth, Stella E
AU  - Autenrieth SE
AD  - Department of Internal Medicine II, University of Tubingen, Tubingen, Germany. 
      Electronic address: Stella.Autenrieth@med.uni-tuebingen.de.
LA  - eng
PT  - Journal Article
DEP - 20160903
PL  - Germany
TA  - Int J Med Microbiol
JT  - International journal of medical microbiology : IJMM
JID - 100898849
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Bacterial Toxins)
RN  - 0 (Toll-Like Receptors)
RN  - 0 (staphylococcal delta toxin)
SB  - IM
MH  - Animals
MH  - Antigens, Bacterial/*immunology
MH  - Bacterial Toxins/*immunology
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - *Immune Tolerance
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Staphylococcus aureus/*immunology
MH  - T-Lymphocytes, Regulatory/*immunology
MH  - Toll-Like Receptors/*metabolism
OTO - NOTNLM
OT  - IL-10
OT  - PSM
OT  - Staphylococcus aureus
OT  - TLR
OT  - Tolerogenic DC
OT  - p38
EDAT- 2016/09/13 06:00
MHDA- 2017/03/09 06:00
CRDT- 2016/09/13 06:00
PHST- 2016/04/20 00:00 [received]
PHST- 2016/08/23 00:00 [revised]
PHST- 2016/09/01 00:00 [accepted]
PHST- 2016/09/13 06:00 [pubmed]
PHST- 2017/03/09 06:00 [medline]
PHST- 2016/09/13 06:00 [entrez]
AID - S1438-4221(16)30242-9 [pii]
AID - 10.1016/j.ijmm.2016.09.002 [doi]
PST - ppublish
SO  - Int J Med Microbiol. 2016 Dec;306(8):666-674. doi: 10.1016/j.ijmm.2016.09.002. 
      Epub 2016 Sep 3.