PMID- 27616359
OWN - NLM
STAT- MEDLINE
DCOM- 20170310
LR  - 20240104
IS  - 1976-670X (Electronic)
IS  - 1976-6696 (Print)
IS  - 1976-6696 (Linking)
VI  - 50
IP  - 1
DP  - 2017 Jan
TI  - CRISPR as a strong gene editing tool.
PG  - 20-24
AB  - Clustered regularly-interspaced short palindromic repeats (CRISPR) is a new and 
      effective genetic editing tool. CRISPR was initially found in bacteria to protect 
      it from virus invasions. In the first step, specific DNA strands of virus are 
      identified by guide RNA that is composed of crRNA and tracrRNA. Then RNAse III is 
      required for producing crRNA from pre-crRNA. In The second step, a 
      crRNA:tracrRNA:Cas9 complex guides RNase III to cleave target DNA. After cleavage 
      of DNA by CRISPR-Cas9, DNA can be fixed by Non- Homologous End Joining (NHEJ) and 
      Homology Directed Repair (HDR). Whereas NHEJ is simple and random, HDR is much 
      more complex and accurate. Gene editing by CRISPR is able to be applied to 
      various biological field such as agriculture and treating genetic diseases in 
      human. [BMB Reports 2017; 50(1): 20-24].
FAU - Shen, Shengfu
AU  - Shen S
AD  - Willston Northampton School, Easthampton, MA 01027, USA.
FAU - Loh, Tiing Jen
AU  - Loh TJ
AD  - School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju 
      61005, Korea.
FAU - Shen, Hongling
AU  - Shen H
AD  - School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju 
      61005, Korea.
FAU - Zheng, Xuexiu
AU  - Zheng X
AD  - School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju 
      61005, Korea.
FAU - Shen, Haihong
AU  - Shen H
AD  - School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju 
      61005, Korea.
LA  - eng
PT  - Journal Article
PT  - Review
PL  - Korea (South)
TA  - BMB Rep
JT  - BMB reports
JID - 101465334
RN  - 0 (CRISPR-Associated Proteins)
RN  - 0 (RNA, Guide, CRISPR-Cas Systems)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - CRISPR-Associated Proteins/*genetics
MH  - *CRISPR-Cas Systems
MH  - Clustered Regularly Interspaced Short Palindromic Repeats/*genetics
MH  - DNA/genetics
MH  - Gene Editing/*methods
MH  - Humans
MH  - RNA Editing/genetics
MH  - RNA, Guide, CRISPR-Cas Systems/genetics
PMC - PMC5319660
EDAT- 2016/09/13 06:00
MHDA- 2017/03/11 06:00
PMCR- 2017/02/01
CRDT- 2016/09/13 06:00
PHST- 2016/07/28 00:00 [received]
PHST- 2016/09/13 06:00 [pubmed]
PHST- 2017/03/11 06:00 [medline]
PHST- 2016/09/13 06:00 [entrez]
PHST- 2017/02/01 00:00 [pmc-release]
AID - 3625 [pii]
AID - bmb-50-020 [pii]
AID - 10.5483/bmbrep.2017.50.1.128 [doi]
PST - ppublish
SO  - BMB Rep. 2017 Jan;50(1):20-24. doi: 10.5483/bmbrep.2017.50.1.128.