PMID- 27616446
OWN - NLM
STAT- MEDLINE
DCOM- 20170330
LR  - 20171008
IS  - 1096-1208 (Electronic)
IS  - 0882-4010 (Linking)
VI  - 100
DP  - 2016 Nov
TI  - The YfcO fimbriae gene enhances adherence and colonization abilities of avian 
      pathogenic Escherichia coli in vivo and in vitro.
PG  - 56-61
LID - S0882-4010(16)30179-6 [pii]
LID - 10.1016/j.micpath.2016.09.007 [doi]
AB  - Chaperone-usher (CU) fimbriae, which are adhesive surface organelles found in 
      many Gram-negative bacteria, mediate tissue tropism through the interaction of 
      fimbrial adhesins with specific receptors expressed on the host cell surface. A 
      CU fimbrial gene yfcO, was identified in avian pathogenic E. coli (APEC) strain 
      DE205B via gene functional analysis. In this study, yfcO was found in 13.41% 
      (11/82) of E. coli strains, including phylogenetic groups A, B1, B2 and D, with 
      the highest percentage in group B2. The expression of yfcO in biofilm forming 
      bacteria was significantly higher (P < 0.05) than that in the planktonic 
      bacteria. A yfcO deletion mutant was constructed, and adherence to DF-1 chicken 
      embryo fibroblast cells was analyzed in vitro. Compared to the wild-type (WT), 
      adherence of the mutant to DF-1 cells was significantly decreased (P < 0.01). The 
      mutant bacterial loads in the heart, brain and liver were significantly lower 
      (P < 0.05) than those of the WT strain. Resistance of the mutant to acidic 
      (acetic, pH 4.0, 20 min) and high osmolarity (2.5 M NaCl, 1 h) stress conditions 
      decreased by 51.28% (P < 0.001) and 80.34% (P < 0.01), respectively. These 
      results suggest that yfcO contributes to APEC virulence through bacterial 
      adherence to host tissues.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Li, Yaxin
AU  - Li Y
AD  - Key Lab of Animal Bacteriology, Ministry of Agriculture, College of Veterinary 
      Medicine, Nanjing Agricultural University, Nanjing 210095, China.
FAU - Wang, Haojin
AU  - Wang H
AD  - Key Lab of Animal Bacteriology, Ministry of Agriculture, College of Veterinary 
      Medicine, Nanjing Agricultural University, Nanjing 210095, China.
FAU - Ren, Jianluan
AU  - Ren J
AD  - Key Lab of Animal Bacteriology, Ministry of Agriculture, College of Veterinary 
      Medicine, Nanjing Agricultural University, Nanjing 210095, China.
FAU - Chen, Ling
AU  - Chen L
AD  - Key Lab of Animal Bacteriology, Ministry of Agriculture, College of Veterinary 
      Medicine, Nanjing Agricultural University, Nanjing 210095, China.
FAU - Zhuge, Xiangkai
AU  - Zhuge X
AD  - Key Lab of Animal Bacteriology, Ministry of Agriculture, College of Veterinary 
      Medicine, Nanjing Agricultural University, Nanjing 210095, China.
FAU - Hu, Lin
AU  - Hu L
AD  - Key Lab of Animal Bacteriology, Ministry of Agriculture, College of Veterinary 
      Medicine, Nanjing Agricultural University, Nanjing 210095, China.
FAU - Li, Dezhi
AU  - Li D
AD  - Key Lab of Animal Bacteriology, Ministry of Agriculture, College of Veterinary 
      Medicine, Nanjing Agricultural University, Nanjing 210095, China.
FAU - Tang, Fang
AU  - Tang F
AD  - Key Lab of Animal Bacteriology, Ministry of Agriculture, College of Veterinary 
      Medicine, Nanjing Agricultural University, Nanjing 210095, China. Electronic 
      address: tangfang@njau.edu.cn.
FAU - Dai, Jianjun
AU  - Dai J
AD  - Key Lab of Animal Bacteriology, Ministry of Agriculture, College of Veterinary 
      Medicine, Nanjing Agricultural University, Nanjing 210095, China.
LA  - eng
PT  - Journal Article
DEP - 20160909
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 0 (Adhesins, Bacterial)
RN  - 0 (Bacterial Outer Membrane Proteins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (YfcO protein, E coli)
RN  - 451W47IQ8X (Sodium Chloride)
SB  - IM
MH  - Adhesins, Bacterial/genetics/*metabolism
MH  - Animal Structures/microbiology
MH  - Animals
MH  - *Bacterial Adhesion
MH  - Bacterial Load
MH  - Bacterial Outer Membrane Proteins/genetics/*metabolism
MH  - Biofilms/growth & development
MH  - Cell Line
MH  - Chickens
MH  - Disease Models, Animal
MH  - Escherichia coli/classification/genetics/*pathogenicity/*physiology
MH  - Escherichia coli Infections/microbiology/*pathology
MH  - Escherichia coli Proteins/genetics/*metabolism
MH  - Fibroblasts/microbiology
MH  - Fimbriae, Bacterial/*physiology
MH  - Gene Deletion
MH  - Genotype
MH  - Sodium Chloride/metabolism
MH  - Virulence
OTO - NOTNLM
OT  - Adhesion
OT  - Avian pathogenic E. coli (APEC)
OT  - Chaperone-usher fimbriae
OT  - Virulence
OT  - yfcO
EDAT- 2016/10/28 06:00
MHDA- 2017/03/31 06:00
CRDT- 2016/09/13 06:00
PHST- 2016/04/09 00:00 [received]
PHST- 2016/09/05 00:00 [revised]
PHST- 2016/09/06 00:00 [accepted]
PHST- 2016/10/28 06:00 [pubmed]
PHST- 2017/03/31 06:00 [medline]
PHST- 2016/09/13 06:00 [entrez]
AID - S0882-4010(16)30179-6 [pii]
AID - 10.1016/j.micpath.2016.09.007 [doi]
PST - ppublish
SO  - Microb Pathog. 2016 Nov;100:56-61. doi: 10.1016/j.micpath.2016.09.007. Epub 2016 
      Sep 9.