PMID- 27617872
OWN - NLM
STAT- MEDLINE
DCOM- 20170718
LR  - 20220331
IS  - 1536-4798 (Electronic)
IS  - 0277-3740 (Linking)
VI  - 36
IP  - 1
DP  - 2017 Jan
TI  - Sex Chromosome Analysis of Postmortem Corneal Endothelium After Sex-Mismatch 
      Descemet Membrane Endothelial Keratoplasty.
PG  - 11-16
AB  - PURPOSE: To identify the origin of corneal endothelial cells (host or donor) 
      present on grafts at various time points after Descemet membrane endothelial 
      keratoplasty (DMEK), using fluorescence in situ hybridization (FISH) of sex 
      chromosomes on post mortem corneas with sex mismatch between the donor and host. 
      METHODS: Corneoscleral buttons of 6 post mortem DMEK eyes of 4 patients, operated 
      for Fuchs endothelial dystrophy, with an average postoperative time of 2.6 (+/-1.8) 
      years (range, 7 months-4.5 years), of 2.5 (+/-1.7) years (range, 7 months-4 years), 
      were processed for FISH detection of XX (female) or XY (male)-labeling signals in 
      corneal endothelial cells in the central area of the DMEK graft. Two male 
      patients underwent bilateral DMEK with grafts from female donors, and 2 female 
      patients underwent unilateral DMEK and received a graft from a male donor. 
      RESULTS: FISH consistently showed the presence of donor endothelial cells across 
      the graft area, with signaling of sex chromosomes opposite to the sex of the 
      host. CONCLUSIONS: Donor endothelial cells may survive up to 4.5 years after 
      DMEK. If so, the lower incidence of allograft rejection in DMEK may not be 
      explained by early host cell replacement. Potential host cell migration may be 
      limited by donor/recipient cell-cell contact inhibition.
FAU - Lavy, Itay
AU  - Lavy I
AD  - *Netherlands Institute for Innovative Ocular Surgery, Rotterdam, The Netherlands; 
      daggerMelles Cornea Clinic Rotterdam, Rotterdam, The Netherlands; double daggerDepartment of 
      Pathology, Section of Ophthalmic Pathology, Erasmus MC University Medical Center, 
      Rotterdam, The Netherlands;  section signGavin Herbert Eye Institute, University of 
      California, Irvine, CA; and  paragraph signAmnitrans EyeBank, Rotterdam, The Netherlands.
FAU - Verdijk, Robert M
AU  - Verdijk RM
FAU - Bruinsma, Marieke
AU  - Bruinsma M
FAU - Sleddens, Hein F
AU  - Sleddens HF
FAU - Oellerich, Silke
AU  - Oellerich S
FAU - Binder, Perry S
AU  - Binder PS
FAU - Melles, Gerrit R J
AU  - Melles GR
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cornea
JT  - Cornea
JID - 8216186
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Cell Survival
MH  - Descemet Membrane/surgery
MH  - Descemet Stripping Endothelial Keratoplasty/*methods
MH  - Endothelial Cells/cytology
MH  - Endothelium, Corneal/*cytology/transplantation
MH  - Female
MH  - Fuchs' Endothelial Dystrophy/*surgery
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - *Sex Chromosomes
MH  - *Sex Factors
MH  - *Tissue Donors
EDAT- 2016/09/13 06:00
MHDA- 2017/07/19 06:00
CRDT- 2016/09/13 06:00
PHST- 2016/09/13 06:00 [pubmed]
PHST- 2017/07/19 06:00 [medline]
PHST- 2016/09/13 06:00 [entrez]
AID - 10.1097/ICO.0000000000001019 [doi]
PST - ppublish
SO  - Cornea. 2017 Jan;36(1):11-16. doi: 10.1097/ICO.0000000000001019.