PMID- 27619333
OWN - NLM
STAT- MEDLINE
DCOM- 20170515
LR  - 20190221
IS  - 1090-2422 (Electronic)
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 348
IP  - 1
DP  - 2016 Oct 15
TI  - Identification, expansion and characterization of cancer cells with stem cell 
      properties from head and neck squamous cell carcinomas.
PG  - 75-86
LID - 10.1016/j.yexcr.2016.09.003 [doi]
AB  - Head and neck squamous cell carcinoma (HNSCC) is a major public health concern. 
      Recent data indicate the presence of cancer stem cells (CSC) in many solid 
      tumors, including HNSCC. Here, we assessed the stem cell (SC) characteristics, 
      including cell surface markers, radioresistance, chromosomal instability, and in 
      vivo tumorigenic capacity of CSC isolated from HNSCC patient specimens. We show 
      that spheroid enrichment of CSC from early and short-term HNSCC cell cultures was 
      associated with increased expression of CD44, CD133, SOX2 and BMI1 compared with 
      normal oral epithelial cells. On immunophenotyping, five of 12 SC/CSC markers 
      were homogenously expressed in all tumor cultures, while one of 12 was negative, 
      four of 12 showed variable expression, and two of the 12 were expressed 
      heterogeneously. We showed that irradiated CSCs survived and retained their 
      self-renewal capacity across different ionizing radiation (IR) regimens. 
      Fluorescence in situ hybridization (FISH) analyses of parental and 
      clonally-derived tumor cells revealed different chromosome copy numbers from cell 
      to cell, suggesting the presence of chromosomal instability in HNSCC CSC. 
      Further, our in vitro and in vivo mouse engraftment studies suggest that 
      CD44+/CD66- is a promising, consistent biomarker combination for HNSCC CSC. 
      Overall, our findings add further evidence to the proposed role of HNSCC CSCs in 
      therapeutic resistance.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Kaseb, Hatem O
AU  - Kaseb HO
AD  - Department of Human Genetics, University of Pittsburgh Graduate School of Public 
      Health, Pittsburgh, PA, 15261, United States of America.
AD  - Department of Clinical Pathology, National Cancer Institute (NCI), Cairo 
      University, Cairo, Egypt.
FAU - Fohrer-Ting, Helene
AU  - Fohrer-Ting H
AD  - Department of Pathology, University of Pittsburgh School of Medicine, 200 Lothrop 
      Street, Pittsburgh, PA, 15261, United States of America.
AD  - McGowan Institute for Regenerative Medicine, University of Pittsburgh, 450 
      Technology Drive, Suite 300, Pittsburgh, PA, 15219, United States of America.
FAU - Lewis, Dale W
AU  - Lewis DW
AD  - Department of Human Genetics, University of Pittsburgh Graduate School of Public 
      Health, Pittsburgh, PA, 15261, United States of America.
FAU - Lagasse, Eric
AU  - Lagasse E
AD  - Department of Pathology, University of Pittsburgh School of Medicine, 200 Lothrop 
      Street, Pittsburgh, PA, 15261, United States of America.
AD  - McGowan Institute for Regenerative Medicine, University of Pittsburgh, 450 
      Technology Drive, Suite 300, Pittsburgh, PA, 15219, United States of America.
FAU - Gollin, Susanne M
AU  - Gollin SM
AD  - Department of Human Genetics, University of Pittsburgh Graduate School of Public 
      Health, Pittsburgh, PA, 15261, United States of America.
AD  - University of Pittsburgh Cancer Institute, Pittsburgh, PA, 15232, United States 
      of America.
LA  - eng
GR  - P30 CA047904/CA/NCI NIH HHS/United States
GR  - P50 CA097190/CA/NCI NIH HHS/United States
PT  - Journal Article
DEP - 20160909
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Antigens, CD)
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Biomarkers, Tumor/metabolism
MH  - Carcinogenesis/pathology
MH  - Carcinoma, Squamous Cell/metabolism/*pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Cell Self Renewal
MH  - Cell Separation
MH  - Chromosomal Instability
MH  - Clone Cells
MH  - Feeder Cells/cytology
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Head and Neck Neoplasms/metabolism/*pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mice
MH  - Middle Aged
MH  - Neoplastic Stem Cells/metabolism/*pathology
MH  - Radiation Tolerance
MH  - Squamous Cell Carcinoma of Head and Neck
PMC - PMC5056028
MID - NIHMS817518
OTO - NOTNLM
OT  - Cancer stem cells
OT  - Cell surface markers
OT  - Chromosomal instability
OT  - Head and neck squamous cell carcinoma
OT  - Radioresistance
EDAT- 2016/09/14 06:00
MHDA- 2017/05/16 06:00
PMCR- 2017/10/15
CRDT- 2016/09/14 06:00
PHST- 2016/04/25 00:00 [received]
PHST- 2016/08/15 00:00 [revised]
PHST- 2016/09/07 00:00 [accepted]
PHST- 2016/09/14 06:00 [pubmed]
PHST- 2017/05/16 06:00 [medline]
PHST- 2016/09/14 06:00 [entrez]
PHST- 2017/10/15 00:00 [pmc-release]
AID - 10.1016/j.yexcr.2016.09.003 [doi]
PST - ppublish
SO  - Exp Cell Res. 2016 Oct 15;348(1):75-86. doi: 10.1016/j.yexcr.2016.09.003. Epub 
      2016 Sep 9.